血培養(yǎng)陽性標(biāo)本直接細(xì)菌鑒定和藥敏分析.doc

血培養(yǎng)陽性標(biāo)本直接細(xì)菌鑒定和藥敏分析 李燕 日照市東港區(qū)人民醫(yī)院檢驗(yàn)科，山東日照276800 摘要目的對(duì)血培養(yǎng)陽性標(biāo)本直接細(xì)菌鑒定和藥敏進(jìn)行分析。方法研究我院收治的發(fā)熱合并全身感染患者86例，在專用接種水中混摻患者的血培養(yǎng)標(biāo)本，并在鑒定板上接種，通過直接法鑒定，同時(shí)把陽性標(biāo)本轉(zhuǎn)種平板進(jìn)行分離培養(yǎng)，根據(jù)常規(guī)法對(duì)分純后的菌落實(shí)施上機(jī)鑒定及藥敏實(shí)驗(yàn)，然后進(jìn)行對(duì)比。結(jié)果在86例樣品當(dāng)中，直接法和常規(guī)法符合率：細(xì)菌鑒定總符合率為96.4%，其中革蘭陰性菌95.8%，革蘭陽性球菌97.1%；兩種方法的細(xì)菌結(jié)果均有著較高的準(zhǔn)確率，細(xì)菌鑒定準(zhǔn)確率在95.7%以上。結(jié)論血培養(yǎng)陽性標(biāo)本直接細(xì)菌鑒定和藥敏實(shí)驗(yàn)?zāi)軌驕?zhǔn)確的早期診斷菌血癥患者，值得推廣。 關(guān)鍵詞血培養(yǎng)；陽性標(biāo)本；細(xì)菌鑒定；藥敏實(shí)驗(yàn) R446A1672-5654（xx）05（c）-0001-02 Directbacterialidentificationandsusceptibilityanalysisofpositivebloodculturespecimens LIYan DepartmentofDonggangRizhaoCityDistrictPeoplesHospital，Rizhao276800,China AbstractObjectiveDirectlyonpositivebloodculturespecimenswereanalyzedbacterialidentificationandsusceptibility.MethodsFeverbinedstudyofsystemicinfectioninourhospital86patientsvainatedinspecialwaterblendingbloodculturespecimensofpatients,andintheidentificationofinoculationplate,identifiedbythedirectmethod,whilethetransferofpositivesampleswereisolatedandculturedspeciestablet,aordingtoconventionalActonthecoloniesaftertheimplementationofapuremachineidentificationandsusceptibilitytesting,andthenpared.ResultsIn86samples,methodfortheidentificationofsusceptibilitydirectlywiththerateof96.4%,thetestresultsofP0.05,nostatisticalsignificance;theresultsofbacteriaidentificationaurac