R-DNMDP-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemE(R)-DNMDPCat.No.:HY-122751CASNo.:1630760-60-9分?式:C??H??N?O?分?量:304.34作?靶點(diǎn):Phosphodiesterase(PDE)作?通路:MetabolicEnzyme/Protease儲(chǔ)存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:100mg/mL(328.58mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM3.2858mL16.4290mL32.8580mL5mM0.6572mL3.2858mL6.5716mL10mM0.3286mL1.6429mL3.2858mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:2.5mg/mL(8.21mM);Suspendedsolution;Needultrasonic1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE2.請(qǐng)依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:2.5mg/mL(8.21mM);Suspendedsolution;NeedultrasonicBIOLOGICALACTIVITY?物活性(R)-DNMDP?種有效的選擇性癌細(xì)胞殺傷劑。(R)-DNMDP，DNMDP的R形式，可以直接結(jié)合PDE3A。(R)-DNMDP?(S)-DNMDP對(duì)的HeLa細(xì)胞系的EC50低500倍。體外研究(R)-DNMDP(0.1pM-1mM;for48hours)hasa500-foldlowerEC50comparedtothe(S)-enantiomerinHeLacellline[1].(S)-DNMDPhasnon-cytotoxic.BindingofPDE3Atothelinkeranalogueisblockedbybothtrequinsinand(R)-DNMDP,butnotbythe(S)-DNMDP[1].REFERENCES[1].deWaalL,etal.Identificationofcancer-cytotoxicmodulatorsofPDE3Abypredictivechemogenomics.NatChemBiol.2016Feb;12(2):102-8.NatChemBiol.2016Feb;12(2):102-8.[2].LucDEWAAL,etal.Compositionsandmethodsforcancerexpressingpde3aorslfn12.WO2017027854A1.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3