INCell用于整孔細胞簇分析

1、IN Cell Analyzer 2000Cluster Phenotype AssayPhenotypic screen to identify compounds which change cluster cells into monolayer cells4x objectiveCorning 3712 384-well plateA selection of 2-color fused whole-well images are displayedDAPI 75msec exposureGFP 1.5sec exposureIN Cell Analyzer 2000 Cluster P

2、henotype Assay4x objective, 384-well plateDAPI whole-well image shown (left) Overlays for nuclei detection using Developer toolbox software (right)IN Cell Analyzer 2000 Cluster Phenotype Assay4x objective, 384-well plateGFP whole-well image shown (left) Overlays for all cell clusters using Developer

3、 toolbox software (right)Clusters defined as having a minimum area 2000 microns2IN Cell Analyzer 2000 Cluster Phenotype Assay4x objective, 384-well plateDAPI-GFP partial-well fused image shown (left) Overlays for cell clusters using Developer toolbox software (right)Small clusters (red) defined as h

4、aving an area of 2000-10,000 microns2Medium clusters (green) defined as having an area of 10,000-100,000 microns2IN Cell Analyzer 2000 Cluster Phenotype Assay4x whole well image (384-well plate)All ClustersSmall Clusters (red)Medium Clusters (green)IN Cell Analyzer 2000 Cluster Phenotype Assay4x who

5、le well image (384-well plate)All ClustersSmall Clusters (red)Medium Clusters (green)Large Clusters (yellow)IN Cell Analyzer 2000 Cluster Phenotype AssaySpotfire trellis plot shows number per well of: All nuclei nuclei in clustersIN Cell Analyzer 2000 Cluster Phenotype AssaySpotfire trellis plot shows number per well of: large clusters medium clusters small clusters IN Cell Analyzer 2000 Cluster Phenotype AssaySpotfir