通用引物序列

1、引物名稱       引物序列3AOX5'-GGC AAA TGG CAT TC TGA CAT-3'3'AD5'-AGA TGG TGC ACG ATG CAC AG-3'3'BD5'-TAA GAG TCA CTT TAA AAT TTG TAT C-3'5AOX15'-GAC TGG TTC CAA TTG ACA AGC-3'A-FACTOR5'-TAC TAT TGC CAG CAT TGC TGC-3'BAC15'-AA

2、C CAT CTC GCA AAT AAA TA-3'BAC25'-ACG CAC AGA ATC TAG CGC TT-3'BGH rev5'-TAG AAG GCA CAG TCG AGG C-3'CMV-F5'-CGC AAA TGG GCG GTA GGC GTG-3'CMV-R5'-GTT CAC GGT GCC CTC C-3'DsRed1-C-r5'-AGC TGG ACA TCA CCT CCC ACA ACG-3'DsRed1-N-f5'-GTA CTG GAA CTG GGG G

3、GA CAG-3'EBV Reverse5'-TGT GGT TTG TCC AAA CTC AAT C-3'EBV-R5'-GTG GTT TGT CCA AAC TCA TC-3'GAL4 AD-F5'-TAC CAC TAC AAT GGA TG-3'GAL4 BD5'-TCA TCG GAA GAG AGT AG-3'GLP15'-TGT ATC TTA TGG TAC TGT AAC TG-3'GLP25'-CTT TAT GTT TTT GGC GTC TTC CA-3'M13(

4、-96)5'-CCC TCA TAG TTA GCG TAA CG-3'M13F5'-TGT AAA ACG ACG GCC AGT-3'M13F（-47）5'-CGC CAG GGT TTTC CCA GTC ACG AC-3'M13R5'-CAG GAA ACA GCT ATG ACC-3'M13R（-48）5'-AGC GGA TAA CAA TTT CAC ACA GGA-3'P249905'-TCATCGGAAGCGAGTAG-3'P314305'-CGT TTT AAAACCTA

5、AGAGTCAC-3'P125845'-TTT TCA GTA TCT ACG AT-3P171105'-TAC CAC TAC AAT GGA TG-3PAS2-1F5'-TCA TCG GAA GCG AGT AG-3'PAS2-1R5'-CGT TTT AAA ACC TAA GAG TCA C-3'PB42ADF5'-CCA GCC TCT TGC TGA GTG GAG ATG-3'PB42ADR5'-AAG CCG ACA ACC TTG ATT GGA G-3'pbd-gal4-f5'

6、-GCC TCT AAC ATT GAG ACA GC-3'pbd-gal4-r5'-AAG AGT TAC TCA AGA ACA AGA A-3'pbi-121(35s)5'-GAC GCA CAA TCC CAC TAT CC-3'PBV220F5'-AAG AAG GGC AGC ATT CAA AG-3'PBV220R5'-CTG CGT TCT GAT TTA ATC TG-3'PCDNA3.0-F25'-CTC TGG CTA ACT AGA GAA CC-3'PCDNA3.0-R25'

7、;-GGC AAC TAG AAG GCA CAG TC-3'PCDNA3.1F5'-CTA GAG AAC CCA CTG CTT AC-3PCDNA3.1R5'-TAG AAG GCA CAG TCG AGG-3pCEP Frorward5'-AGC AGA GCT CGT TTA GTG AAC CG-3'PCEP-F5'-AGA GCT CGT TTA GTG AAC CG-3'PCMV-F5'-TCT AAA AGC TGC GGA ATT GT-3'PCMV-F5'-TTC CAA AAT GTC GT

8、A ATA AC-3'PCMV-R5'-TCC AAA CTC ATC AAT GTA TC-3'PCMV-R5'-ATT ATA GAG GAC ACC TAG TC-3'PDONR-F5'-TCG CGT TAA CGC TAG CAT GGA TCT C-3'PDONR-R5'-GTA ACA TCA GAG ATT TTG AGA CAC-3PEF-F5'-TCA AGC CTC AGA CAG TGG TTC-3'PEGFP-C-35'-TAT GGC TGA TTA TGA TCA GT-3&#

9、39;PEGFP-C-55'-CAT GGT CCT GCT GGA GTT CGT G-3'PEGFP-N-35'-CGT CGC CGT CCA GCT CGA CCA G-3'PEGFP-N-55'-TGG GAG GTC TAT ATA AGC AGA G-3'pETup5'-ACG ATG CGT CCG GCG TAG AGG A-3'PGEX-35'-CCG GGA GCT GCA TGT GTC AGA GG-3'PGEX-55'-GGG CTG GCA AGC CAC GTT TGG TG

10、-3'PIRES2-EGFP-F5'-GTA GGC GTG TAC GGT GGG AG-3'PIRES3-EGFP-R5'-AAC GCA CAC CGG CCT TAT TC-3'pMAL-C2X-F5'-TGC GTA CTG CGG TGA TCA AC-3pMAL-C2X-R5'-CTG CAA GGC GAT TAA GTT GG-3PQE30F5'-TGA GCG GAT AAC AAT TTC AC-3'PQE30R5'-GTT CTG AGG TCA TTA CTG G-3'pTARGE

11、T-SEQ5'-TTA CGC CAA GTT ATT TAG GTG ACA-3PTRC99C-F5'-TTG CGC CGA CAT CAT AAC-3PTRC99C-R5'-CTG CGT TCT GAT TTA ATC TG-3PTRCHISA-F5'-CAT GGT ATG GCT AGC ATG AC-3PTRCHISA-R5'-CAG GCT GAA AAT CTT CTC TC-3RVP35'-CTA GCA AAA TAG GCT GTC CC-3'RVP45'-GAC GAT AGT CAT GCC CCG C

12、G-3'S.TAG5'-CGA ACG CCA GCA CAT GGA CA-3'S15'-CAA CGT GAA AAA ATT ATT ATT CGC-3S65'-GTA AAT GAA TTT TCT GTA TGA GG-3SP65'-ATT TAG GTG ACA CTA TAG-3'T35'-ATT AAC CCT CAC TAA AGG GA-3'T75'-TAA TAC GAC TCA CTA TAG GG-3'T7（17BASE）5'-ACA TCC ACT TTG CCT TTC

13、 TC-3'T7TER5'-TGC TAG TTA TTG CTC AGC GG-3'TRXF5'-CAT ATG AGC GAT AAA ATT ATT CAC-3'TRXR5'-GGA TCC CTT GTC ATC GTC ATC ACC A-3'malEfor5'-GGT CGT CAG ACT GTC GAT GAA GCC-3'pTrcHis-fw5'-TTA AAG AGG TAT ATA TTA ATG TAT CG-3'Xpressfor5'-AGC ATG ACT GGT GGA

14、 CAG-3'pTrcHis-rv5'-ATC TGT ATC AGG CTG AAA ATC-3'N-CMV-305'-ATA ACC CCG CCC CGT TG-3'C-CMV-245'-TTA GGA CAA GGC TGG TGG-3'CMV-Profor5'-ATG GGC GGT AGG CGT G-3'pCAMBIA1301-5'(ECORI)5'-CCA GGC TTT ACA CTT TAT GC-3'pCAMBIA1301-3'(HindIII)5'-GCG A

15、TT AAG TTG GGT AAC GC-3'T7 EEV5'-ATG TCG TAA TAA CCC CGC CCC G-3'pDBLeu-5'5'-GAA TAA GTG CGA CAT CAT CAT C- 3pDBLeu-3'5'-CAG ATG GAA CGG TCT TTA AAT G-3HGHrev5'-CTG GGG AGG GGT CAC AGG G-3'PSG5(BAMHI)-F5'-GCT GGT TAT TGT GCT GTC TC-3'PSG5-R(SV40)5'-AAA

16、 CCA CAA CTA GAA TGC AGT-3'M13R(-26)5'-CAG GAA ACA GCT ATG AC-3'pBRforBam5'-CTT GGA GCC ACT ATC GAC-3'pBRrevBam5'-GGT GAT GTC GGC GAT ATA GG-3'pBRforEco5'-AAT AGG CGT ATC ACG AGG C-3'pBRrevHind5'-GCG TTA GCA ATT TAA CTG TG-3' c-mycrev5'-CTC TTC TGA GAT

17、 GAG TTT TTG-3' pGAL4-ADrv5'-GAA CTT GCG GGG TTT TTC-3' pGAL4-BDrv5'-AAT CAT AAG AAA TTC GCC C-3' pMD19T490R5'-CACAGGAAACAGCTATGACC-3'Psos5'5'-CCA AGA CCA GGT ACC ATG-3'Psos3'5'-GCC AGG GTT TTC CCA GT-3'Primer TriplEx-LD-5´ 5'-CTC GGG AAG

18、CGC GCC ATT GTG TTG G-3' Primer TriplEx-LDrv-3' 5'-ATA CGA CTC ACT ATA GGG CGA ATT GGC-3' UB-F5'-TCA GTG TTA GAC TAG TAA ATT-3'27F5'-AGA GTT TGA TCC TGG CTC AG-31492R5'-GGY TAC CTT GTT ACG ACT T-3PTR55'-CTC CGA GAT CTG GAC GAG C-3'pLXSN-FCTT GAA CCT CCT C

19、GT TCG ACpLXSN-RGTT GCT GAC TAA TTG AGA TGpLEXA-FCGT CAG CAG AGC TTC ACC ATpLEXA-RTAA AAC CTA AGA GTC ACT TTpLNCX-FAGC TCG TTT AGT GAA CCG TCA GAT CGpLNCX-RACC TAC AGG TGG GGT CTT TCA TTC CCpDSRED-N-RTGA AGC GCA TGA ACT CCT TGmtDNA-D-LoopFAGG ACT Acg gct tga aaa gcmtDNA-D-LoopRatg tgc ctg acc gag ga

20、a cca gH1primerCGCTATGTGTTCTGGGAAATpRECEIVER-M03F5'-GCGGTAGGCGTGTACGGT-3'pRECEIVER-M03R5'-CCGGACACGCTGAACTTGT-3'ACYCDuetUP1Primer #71178-3GGATCTCGACGCTCTCCCTDuetUP2 PrimerTTGTACACGGCCGCATAATCDuetDOWN1GATTATGCGGCCGTGTACAA1525R5'-AgAAAggAggTgATCcAgcc-3'pBAD_FATGCCATAGCATTTTTATCC pBAD_RGATTTAATCTGTATCAGGAUG1F5'-CAATTTACATCTTTATTTATTAACG-3'AUG1R5'-GAAGAGAAAAACATTAGTTGGC-3'V5rev 5'-ATC GAG ACC GAG GAG AGG -3'EGFP-Nrev5'-CGT CGC CGT CCA GCT C-3'TKPAreverse5?-CTTCCGTGTTTCAGTTAGC-3?p