肺癌免疫治療進(jìn)展專家講座

肺癌免疫治療進(jìn)展肺癌免疫治療進(jìn)展第1頁(yè)FutureOutlookUpdateofcheckpointInhibitorsinlungcancertherapyCancerImmunotherapy123Outline肺癌免疫治療進(jìn)展第2頁(yè)FutureOutlookUpdateofcheckpointInhibitorsinlungcancertherapyCancerImmunotherapy123Outline肺癌免疫治療進(jìn)展第3頁(yè)

腫瘤免疫治療—攻克腫瘤新希望

人類抗擊腫瘤歷史腫瘤免疫治療含有特異性和靶向性，一直為臨床醫(yī)師高度關(guān)注，近年進(jìn)步顯著，使得免疫治療成為更具期待領(lǐng)域1896年coley毒素應(yīng)用于臨床1899年放療治愈第1例病人1946年氮芥治療淋巴瘤獲得成功免疫治療放療化療靶向治療進(jìn)入21世紀(jì)，分子靶向治療如火如荼肺癌免疫治療進(jìn)展第4頁(yè)eKeyeventsinthehistoryofcancerimmunotherapy1890s1stCAvaccinedeveloped(coley)1973discoveryofthedendriticcell(steinman)19761ststudywithBCGinbladderCA1978DiscoveryoftumorspecificmABs19851ststudywithadoptiveT-celltransferinCA1986IFNα(cytokine)approvedforCA1990sDiscoveryofroleofcheckpointsinCA1992Il-2(Cytokine)approvedforCA19971stmABapprovedforCA1stcellularimmunotherapyapprovedforCA1stcheckpointinhibitorapprovedforCA2ndcheckpointinhibitorapprovedforCAEnthusiasmphase1976-1985Skepticismphase1986-1992Renaissancephase1997-肺癌免疫治療進(jìn)展第5頁(yè)美國(guó)《Science》雜志:年六大值得關(guān)注科學(xué)領(lǐng)域單細(xì)胞測(cè)序“普朗克”探測(cè)微波背景輻射人類連接組計(jì)劃探索南極冰下世界癌癥免疫療法基礎(chǔ)植物研究肺癌免疫治療進(jìn)展第6頁(yè)Breakthroughofyear

Science.Dec20;342(6165):1432-3肺癌免疫治療進(jìn)展第7頁(yè)Immunity.39(1)25July,Pages1–10StimulatoryandInhibitoryFactorsintheCancer-ImmunityCycle肺癌免疫治療進(jìn)展第8頁(yè)CTLA-4andPD-1/PD-L1checkpoint

blockadeforcancertreatment肺癌免疫治療進(jìn)展第9頁(yè)CTLA-4andPD-1/PD-L1

CheckpointBlockadeforCancerTreatmentImmunecheckpointblockadeincludesagentstargetingthenegativeregulatorsCTLA-4andPD-1CTLA-4attenuatestheearlyactivationofnaiveandmemoryTcellsinthelymphnodes

AgentstargetingCTLA-4includeipilimumabandtremelimumabIncontrast,PD-1modulatestheeffectorphaseofTcellactivityinperipheraltissuesviainteractionwithPD-L1andPD-L2AgentstargetingPD-1includenivolumabandMK-3475

AgentstargetingPD-L1includeMPDL3280AandMEDI4736KyiC,etal.FEBSLett.;588:368-376肺癌免疫治療進(jìn)展第10頁(yè)ComparingCTLA-4andPD-1CTLA-4PD-1BiologicalfunctionInhibitoryreceptorInhibitoryreceptorExpressiononTcellsatthetimeofinitialresponsetoantigen(activatedCD8+Tcells)ActivatedTcells,Bcells,NKcellsTILsindifferenttumortypesMajorroleRegulatestheearlystageofT-cellactivationLimitsT-cellactivityinperipheraltissueafterinflammatoryresponseLimitsautoimmunityLigandsB7.1(CD80)B7.2(CD86)PD-L1(B7-H1/CD274)PD-L2(B7-CD/CD273)MechanismofactionAfterligandbinding:BindingwithPI3K,phosphatasesSHP-2andPP2ABlockadeoflipid-raftexpressionBlockadeofmicroclusterformationAfterligandbinding:Recruitsinhibitoryphosphatase,SHP-2DecreasesexpressionofcellsurvivalproteinBcl-xLInhibitskinases(PI3K/AKT)involvedinT-cellactivationCritRevOncolHematol.;89:140-165.CTLA-4andPD-1haveseparatebutcomplimentaryrolesinimmuneresponses肺癌免疫治療進(jìn)展第11頁(yè)FutureOutlookUpdateofcheckpointInhibitorsinlungcancertherapyCancerImmunotherapy123Outline肺癌免疫治療進(jìn)展第12頁(yè)CTLA-4CheckpointInhibitor肺癌免疫治療進(jìn)展第13頁(yè)Anti-CTLA-4antibodiescaninduce

clinicalresponseinabroadvarietyofcancerAdaptedformLebbeetal.ESMOPresentedByLawrenceFongatASCOAnnualMeetingBladderRenalEsophagealCNSColorectalGlioblastomaLeukemiaSoftTissueSarcoma肺癌免疫治療進(jìn)展第14頁(yè)JClinOncol.Jun10;30(17):2046-54AnnOncol.Jan;24(1):75-83肺癌免疫治療進(jìn)展第15頁(yè)JClinOncol.Jun10;30(17):2046-54IpilimumabincombinationwithPCasfirst-linetherapyinstageIIIB/IVNSCLC肺癌免疫治療進(jìn)展第16頁(yè)Kaplan–MeierplotsforOSJClinOncol.Jun10;30(17):2046-54Deaths/patients51/6651/68Median(95%CI),months8.28(6.80to12.39)12.22(9.26to14.39)HR(95%CI)

0.87

(0.59

to

1.28)Log-rankP0.23ControlPhasedIpiDeaths/patients51/6651/70Median(95%CI),months8.28(6.80to12.39)9.69(7.59to12.48)HR(95%CI)

0.99(0.67to1.46)Log-rankP0.48ConcurrentlpiControl肺癌免疫治療進(jìn)展第17頁(yè)Events/patients61/6658/70Median(95%CI),mo4.21(2.76to5.32)4.11(2.76to5.32)HR(95%CI)

0.88(0.61to1.27)Log-rankP.25JClinOncol.Jun10;30(17):2046-54Kaplan–MeierplotsforPFSperimmune-related(ir)responsecriteria(irPFS)andmodifiedWHOcriteria(mWHO-PFS).Events/patients56/6654/68Median(95%CI),4.63m(4.14to5.52)5.68(4.76to7.79)HR(95%CI)

0.72

(0.50to1.06)Log-rankP.05ControlPhasedIpiEvents/patients56/6655/70Median(95%CI),4.63m(4.14to5.52)5.52(4.17to6.74)HR(95%CI)

0.81(0.55to1.17)Log-rankP.13ControlConcurrentlpiEvents/patients61/6656/68Median(95%CI),mo4.21(2.76to5.32)5.13(4.17to5.72)HR(95%CI)

0.69(0.48to1.00)Log-rankP.02ControlPhasedIpiControlConcurrentlpi肺癌免疫治療進(jìn)展第18頁(yè)AdverseEventsJClinOncol.Jun10;30(17):2046-54肺癌免疫治療進(jìn)展第19頁(yè)Follow-UPEvery12wksForsurvivalSCREENINGINDUCTIONMAINTENANCEFOLLOW-UPCA184-104:phaseIIItrialcomparingthetheefficacyofipilimumab(Ipi)withPCversusplacebowithPCinpatients(pts)withstageIV/recurrentNSCLCofsquamoushistologyTumorassessmentEvery12wksIpi10mg/kg+PCWks7,10,13,16stageIV/recurrentsquamousNSCLCECOG≤1Placebo+PCWks7,10,13,162cyclePC

Wks1,

4Ipi10mg/kgEvery12wksPlaceboEvery12wksRJClinOncol31,(suppl;abstrTPS8117)primaryendpointOSsecondaryendpointsOSamongptswhoreceiveblindedtherapyPFSbestoverallresponserateTumorassessmentWks7,13,19,25ExclusionCriteria:BrainMetastasesAutoimmunediseasesPCPaclitaxel(175mg/m2,

IV)+Carboplatin(AUC=6,

IV)肺癌免疫治療進(jìn)展第20頁(yè)CA184-156:PhaseIIITrialComparingtheEfficacyofIpiPlusEtoposide/PlatinumVersusEtoposide/PlatinuminSubjectsWithNewlyDiagnosedED-SCLCJClinOncol30,(suppl;abstrTPS7113)Ipi+EPQ3W2cycleED-SCLCECOG0-1Placebo+EPQ3W2cycleSCREENINGINDUCTIONMAINTENANCE2cycleEP

Ipi10mg/kgQ12WPlaceboQ12WRprimaryendpointOSsecondaryendpointsOSamongptswhoreceiveblindedtherapyimmune-relatedandmWHOPFSbestoverallresponseratedurationofresponseExclusionCriteria:PriorsystemictherapyforlungcancerSymptomaticCNSmetastasesHistoryofautoimmunediseaseIpiQ3W2cycleEP：etoposide(100mg/m^2,IVonDays1-3Q3W)+cisplatin(75mg/m^2,IV)or+carboplatin(AUC=5,IV)onceQ3WIpi:(10mg/kg,IV,Q3W)PlaceboQ3W2cycle肺癌免疫治療進(jìn)展第21頁(yè)APhaseIIIStudyofNivolumabinCombinationwithYervoyinPatientswithAdvancedNon-SmallCellLungCancer肺癌免疫治療進(jìn)展第22頁(yè)P(yáng)D-1/PD-L1CheckpointInhibitors肺癌免疫治療進(jìn)展第23頁(yè)P(yáng)D-1andPD-L1antibodiesinphaseIIIdevelopment肺癌免疫治療進(jìn)展第24頁(yè)P(yáng)hase1Nivolumab(anti-PD-1;BMS-936558,ONO-4538)multidoseregimenEligibility:advcancedmelanoma,NSCLC,RCC,CRC,orCRPCwithPDafter1-5systemictherapies肺癌免疫治療進(jìn)展第25頁(yè)SelectAes(>1%)occuringinPtswithNSCLCtreatedwithNivolumab(N=129)Drug-relatedpneumonitis(anygrade)occurredin8NSCLCPts(6%)VS12Pts(4%)intheoverallstudypopulation-3Pts(2%)withNSCLChadgrade?pneumonitis肺癌免疫治療進(jìn)展第26頁(yè)EfficacyofNivolumabmonotherapy

inPtstreatedwithNSCLC肺癌免疫治療進(jìn)展第27頁(yè)NivolumabincombinationwithPT-DCinadvancedNSCLCAntoniaSJ,etal.ASCOAbstract8113.肺癌免疫治療進(jìn)展第28頁(yè)ResultsandConclusions治療前6周沒(méi)有發(fā)生劑量限制毒性3-4級(jí)治療相關(guān)不良事件發(fā)生率為45%ORR：33-50%1年OS：59-87%Nivo10+gem/cis鱗癌Nivo10+pem/cis非鱗癌Nivo10+pac/carb鱗+非鱗癌Nivo5+pac/carb鱗+非鱗癌N12151514ORR,n(%)4(33)7(47)7(47)7(50)mDOR(范圍)，周20.9(12.1-41.7)32.0(13,1-42.1)25.6(11.4-39.0)NA(11.4-37.3)PD為BOR,n(%)003(20)1(7)24周時(shí)PFS,%367138571年OS,%598759NAAntoniaSJ,etal.ASCOAbstract8113.AntoniaSJ,etal.ASCOAbstract8113.肺癌免疫治療進(jìn)展第29頁(yè)OngoingNivolumabClinical

TrialsinPatientsWithNSCLCLineoftherapyPhasePD-L1SelectionComparatorSingleagentNivolumab1stline[1]IIIYesChemotherapy2ndline,squamous[2]IIINoDocetaxel2ndline,adeno[3]IIIYesDocetaxel≥2ndline,squamous[4]IINoNACombinationNivolumab≥2ndline[5]INo+LAG3≥2ndline[6]INo+lirilumab(KIR)1stline[7]INoSingleagent;+chemotherapy;+bevacizumab;+erlotinib;+ipilimumabClinicalT.NCT02041533.2.ClinicalT.NCT0164.3.ClinicalT.NCT01673867.4.ClinicalT.NCT01721759.5.ClinicalT.NCT01968109.6.ClinicalT.NCT01714739.7.ClinicalT.NCT01454102.肺癌免疫治療進(jìn)展第30頁(yè)P(yáng)artsCtoF:AdditionalMELandNSCLCcohortsMK3475(Pembrolizumab,Anti-PD-1):

PhaseITrialDesignAprNovDecJanFebMarAprMayJunJulAugSepOctNovDecIPI-N10q2w

(n=41)IPI-N10q3w

(n=24)PartA:DoseEscalationIPI-N2q3w

(n=22)IPI-T10q2w

(n=16)IPI-T10q3w

(n=32)PartB:Metastaticorlocallyadvanced,unresectableMELRibasAetal.ASCO.Abstract9009.肺癌免疫治療進(jìn)展第31頁(yè)KEYNOTE-001：

NSCLC擴(kuò)大隊(duì)列研究設(shè)計(jì)(N=307)非隨機(jī)(N=33)PD-L1+2次治療非隨機(jī)(N=40)PD-L1+2次治療最少1次含鉑隨機(jī)(N=144)PD-L1+1次治療最少1次含鉑隨機(jī)(N=45)PD-L1+初治非隨機(jī)(N=45)PD-L1+1次治療最少1次含鉑Pembro10mg/kgq3wPembro10mg/kgq2wPembro10mg/kgq3wPembro10mg/kgq2wPembro10mg/kgq2wPembro10mg/kgq3wPembro2mg/kgq3wPembro2mg/kgq3wR(3:2)R*(1:1:1)*前11例患者隨機(jī)分入2mg/kgq3w和10mg/kgq3w組，剩下34例患者隨機(jī)接收10mg/kgq2w和10mg/kgq3w組****非隨機(jī)隊(duì)列45例接收2mg/kgq3w患者分析截止日期為年9月11日數(shù)據(jù)截止日期：年3月3日GaronEB,etal.ESMOAbstractLBA43.主要終點(diǎn)：ORR(RECISTv1.1[獨(dú)立中心評(píng)定])次要終點(diǎn)：免疫相關(guān)療效標(biāo)準(zhǔn)(irRC)[研究者評(píng)定]Pembrolizumab(MK3475)治療連續(xù)直至PD，不可接收毒性或死亡肺癌免疫治療進(jìn)展第32頁(yè)KEYNOTE-001：基線特征特征

N=262年紀(jì)，中位(范圍)，歲65(28-86)男性50%ECOGPS：0/1/缺失31%/68%/1%人種：白種/黑人或非裔美國(guó)人/亞裔/其它83%/4%/11%/2%鱗癌17%既往接收治療次數(shù)：0/>=117%/83%分期：M0/M1a/M1b/未知13%/28%/49%/11%腦轉(zhuǎn)移瘤史5%EGFR突變(N=250)16%KRAS突變(N=156)26%ALK基因重排(N=231)3%吸煙史：當(dāng)前/曾經(jīng)/從不/未知5%/64%/28%/2%GaronEB,etal.ESMOAbstractLBA43.肺癌免疫治療進(jìn)展第33頁(yè)KEYNOTE-001：

治療暴露與治療相關(guān)不良事件匯總4例患者(1.5%)發(fā)生輸注相關(guān)反應(yīng)發(fā)生率<1%其它潛在免疫調(diào)整不良事件為結(jié)腸炎和低鈉血癥治療暴露N=262中位(范圍)治療時(shí)間(d)85.5(1-400)中位(范圍)劑量(n)5.5(1-23)治療相關(guān)不良事件總結(jié)(%)任何級(jí)別67%3-4級(jí)9%死亡0.4%終止3%不良事件發(fā)生率N=262任何級(jí)別3-5級(jí)治療相關(guān)不良事件(發(fā)生率≥5%)乏力20%<1%瘙癢9%0關(guān)節(jié)痛8%<1%食欲減退8%0腹瀉7%0甲狀腺功效減退6%0發(fā)燒6%0皮疹6%0惡心5%<1%其它關(guān)注臨床不良事件(發(fā)生率≥1%)肺炎4%2%甲狀腺功效亢進(jìn)2%<1%GaronEB,etal.ESMOAbstractLBA43.肺癌免疫治療進(jìn)展第34頁(yè)KEYNOTE-001：

腫瘤大小自基線最大改變*(%)

(RECISTv1.1，中心評(píng)定)*可評(píng)定患者為依據(jù)中心評(píng)定基線有可測(cè)量病灶且最少接收一次基線后腫瘤評(píng)定GaronEB,etal.ESMOAbstractLBA43.肺癌免疫治療進(jìn)展第35頁(yè)KEYNOTE-001：抗腫瘤活性

(RECISTv1.1，中心評(píng)定)a包含確認(rèn)和未確認(rèn)緩解；b數(shù)據(jù)截止日期為年3月3日GaronEB,etal.ESMOAbstractLBA43.

NORR%(95%CI)總計(jì)23621(16-27)治療史236未經(jīng)治療4226(14-42)曾接收過(guò)治療19420(15-26)組織學(xué)230非鱗癌19123(17-29)鱗癌3918(8-34)吸煙史230當(dāng)前/曾經(jīng)16527(20-34)從不659(4-19)

NORR%(95%CI)給藥方案2362Q3W633(4-78)10Q3W12621(14-29)10Q2W10421(14-30)PD-L1表示236陽(yáng)性20123(18-30)陰性359(2-23)EGFR突變3614(5-30)KRAS突變3928(15-45)ALK基因重排617(0-64)肺癌免疫治療進(jìn)展第36頁(yè)KEYNOTE-001：

抗腫瘤活性(irRC，研究者評(píng)定)a包含確認(rèn)和未確認(rèn)緩解；b數(shù)據(jù)截止日期為年9月11日GaronEB,etal.ESMOAbstractLBA43.額外45例接收2mg/kgq3w治療患者中，ORRa為20%(95%CI:10%-35%)bNORR%(95%CI)總計(jì)26223(18-29)治療史262未經(jīng)治療4547(32-62)曾接收過(guò)治療21718(13-24)組織學(xué)258非鱗癌21223(17-29)鱗癌4425(13-40)吸煙史256當(dāng)前/曾經(jīng)18227(21-34)從不7414(7-24)NORR%(95%CI)給藥方案2622Q3W667(22-96)10Q3W14122(16-30)10Q2W11522(15-30)PD-L1表示262陽(yáng)性22225(19-31)陰性4013(4-27)EGRFR突變4112(4-26)KRAS突變4132(18-48)ALK重排633(4-78)肺癌免疫治療進(jìn)展第37頁(yè)KEYNOTE-001：

至緩解時(shí)間&緩解連續(xù)時(shí)間a包含確認(rèn)和未確認(rèn)緩解GaronEB,etal.ESMOAbstractLBA43.肺癌免疫治療進(jìn)展第38頁(yè)KEYNOTE-001：

生存期評(píng)定：初治vs.復(fù)治GaronEB,etal.ESMOAbstractLBA43.初治復(fù)治中位PFS(周)271024周PFS(%)5126初治復(fù)治中位OS(月)NR8.26個(gè)月OS(%)8659肺癌免疫治療進(jìn)展第39頁(yè)KEYNOTE-001：

生存期評(píng)定：不一樣劑量GaronEB,etal.ESMOAbstractLBA43.全組人群中位PFS(周)13.024周PFS(%)30全組人群中位OS(月)8.26個(gè)月OS(%)64肺癌免疫治療進(jìn)展第40頁(yè)KEYNOTE-001：

PD-L1表示水平與緩解率GaronEB,etal.ESMOAbstractLBA43.肺癌免疫治療進(jìn)展第41頁(yè)KEYNOTE-001：

生存期評(píng)定：PD-L1表示PD-L1強(qiáng)陽(yáng)性：>=50%腫瘤細(xì)胞PD-L1弱陽(yáng)性：1-49%腫瘤細(xì)胞染色陰性為PD-L1無(wú)表示GaronEB,etal.ESMOAbstractLBA43.PD-L1強(qiáng)陽(yáng)性患者較弱陽(yáng)性/陰性患者PFS更長(zhǎng)(HR=0.52;95%CI:0.33-0.80)PD-L1強(qiáng)陽(yáng)性患者較弱陽(yáng)性/陰性患者OS更長(zhǎng)(HR=0.59;95%CI:0.35-0.99)肺癌免疫治療進(jìn)展第42頁(yè)KEYNOTE-001：

總結(jié)與結(jié)論在初治(ORR26%)和復(fù)治(ORR20%)晚期NSCLC患者中，全部劑量和方案都觀察到很好抗腫瘤活性2mg/kgq3w劑量下，ORR為20%(irRC)緩解持久安全性及毒性可管理PD-L1強(qiáng)表示與緩解率(37%)、PFS(HR=0.52)、OS(HR=0.59)改進(jìn)相關(guān)在KEYNOTE-001研究額外入組300例患者中將前瞻性驗(yàn)證PD-L1截點(diǎn)GaronEB,etal.ESMOAbstractLBA43.肺癌免疫治療進(jìn)展第43頁(yè)4/49PD-L1IdentifiesPtsWithNSCLCMostLikelytoBenefitFromMK-3475(Pembrolizumab,Anti-PD-1)StrongPD-L1positivestainingwasconsidered≥50%oftumorcells,andweakwasdefinedasstainingbetween1%to49%ofpositivelystainingtumorcells.NegativehadnotumorstainingforPD-L1.ResponseRate(%)3/427/4615/4125/129GandhiL,etal.AACR.AbstractCT105.Reprintedwithpermission.RR-RECIST1.1504030201001937157Total1%-49%PD-L1staining≥50%PD-L1stainingPD-L1negativeResponseRate(%)4/5320/4428/146RR-irRC50403020100194688n/N:n/N:肺癌免疫治療進(jìn)展第44頁(yè)OngoingMK-3475(Pembrolizumab,Anti-PD-1)

ClinicalTrialsinPatientsWithNSCLCLineofTherapyPhasePD-L1SelectionComparatorSingle-agentMK-34751stline;≥2ndline[1,2]I/IIBothNA2ndline[3]IIIYesDocetaxel1stline[4]IIIYesChemotherapyCombinationMK-3475NA[5]I/IINoSingleagent;+chemotherapy;+pemetrexed;+gefitinib;+erlotinib;+ipilimumab1.ClinicalT.NCT02085070.2.ClinicalT.NCT02129556.3.ClinicalT.NCT01905657.4.ClinicalT.NCT02142738.5.ClinicalT.NCT02039674.肺癌免疫治療進(jìn)展第45頁(yè)ExamplesofPD-L1NSCLC

SampleIHCStaining*PD-L1NegativePD-L1Positive*Clinicaltrialassay.StainingIntensity0+1+2+3+PD-L1Positivity,%02100100GandhiL,etal.AACR.AbstractCT105.Reprintedwithpermission.肺癌免疫治療進(jìn)展第46頁(yè)P(yáng)haseIStudyofMPDL3280A

(Anti-PDL-1)inNSCLCMPDL3280A:anti–PD-L1antibodyengineeredforenhancedsafetyandefficacyPatientswithmetastaticsolidtumorsEGFRandKRASstatusassessedatbaselineStudydesign:MPDL3280AIVevery3wksx16cycles(≈1yr)Primaryendpoint:safetySecondaryendpoint:ORRbyRECISTv1.1BaselinedemographicsCharacteristicsn=85*Medianage,yrs(range)60(24-84)Sex,male/female,n(%)48(56)/37(44)ECOGPS,0/1,n(%)27(32)/58(68)Histology,n(%)Squamous20(24)Nonsquamous65(76)*Safetyevaluablepatients(n=85)withNSCLC.DatacutoffApril30,.?Systemicregimensadministeredinthemetastatic,adjuvantorneoadjuvantsetting.3%ofpatientshadnoprevioussystemicregimens.Characteristics,n(%)n=85*Previoussystemicregimens?1or236(42)≥347(55)SmokingstatusCurrent/previous68(80)Never17(20)HornL,etal.WCLC.AbstractMO18.Reprintedwithpermission.肺癌免疫治療進(jìn)展第47頁(yè)P(yáng)D-L1Status*(N=53)ORR,?%(n/N)PtsWithPD,%(n/N)

IHC3(n=6)83(5/6)17(1/6)IHC2and3(n=13)46(6/13)23(3/13)IHC1/2/3(n=26)31(8/26)38(10/26)Allpatients(IHC0/1/2/3and7patientswithdiagnosticunknown;

N=53)23

(12/53)40

(21/53)DurationofTreatmentandResponseWkHistologyIHCNSIHC0SIHC3NSIHC0NSIHC1NSIHC0SIHC2NSIHC3SIHC3NSIHC3NSIHC0NSIHC3NSIHC1*PD-LIstatusdeterminedusingproprietaryGenentechRocheIHC.?ORRincludesinvestigator-assessedunconfirmedandconfirmed(u/c)PRperRECIST1.1.Patientsfirstdosedat1-20mg/kgbyOctober1,.DatacutoffApril30,.MPDL3280A(Anti-PDL-1)inNSCLC:BestResponsebyPD-L1StatusandDOT/DORHornL,etal.WCLC.AbstractMO18.Reprintedwithpermission.0612182430364248546066727884Onstudy,ontreatment

Onstudy,posttreatment

Treatmentdiscontinued

Ongoingresponse

FirstresponseFirstPD肺癌免疫治療進(jìn)展第48頁(yè)*ORRincludesinvestigator-assessedu/cPRbyRECIST1.1.Patientsfirstdosedat1-20mg/kgbyOctober1,.DatacutoffApril30,.Former/

CurrentSmokersNever

SmokersResponsebySmokingStatus(ORR*)SmokingStatus(NSCLC;n=53)PtsWithPR(%)EGFRMutantEGFRStatus(NSCLC;n=53)UnknownResponsebyEGFRStatus(ORR*)PtsWithPR(%)KRASStatus(NSCLC;n=53)ResponsebyKRASStatus(ORR*)PtsWithPR(%)KRASMutantUnknownEGFRWTEGFRMutantKRASWTKRASMutant11/431/109/401/68/271/10MPDL3280A(Anti-PDL-1)PhaseIa:

ResponsebySmokingandMutationalStatusHornL,etal.WCLC.AbstractMO18.Reprintedwithpermission.504030201005040302010050403020100Former/CurrentSmokersNeverSmokers26%10%23%17%30%10%51%30%19%76%13%11%81%19%KRASWTEGFRWT肺癌免疫治療進(jìn)展第49頁(yè)MajorityofAEsweregrade1/2anddidnotrequireinterventionNoMTDordose-limitingtoxicitiesNograde3-5pneumonitisobservedTreatment-relateddeath(cardio-respiratoryarrest)in1patientwithsinusthrombosisandlargetumormassinvadingtheheartatbaselineImmune-relatedgrade3.4AEs:1patientwithlarge-cellneuroendocrineNSCLC(diabetesmellitus,1%)MPDL3280A(Anti-PDL-1):Treatment-RelatedAdverseEventsinPatientsWithNSCLC*AEsoccurringin≥5%ofpatients.?Grade3/4treatment-relatedAEslistedincludetreatment-relatedAEsforwhichtheanygradeoccurrencewas≥5%ofpatients.DatacutoffApril30,.AdverseEvent(n=85)TreatmentRelated,%(n)AnyGrade*Grade3/4?AnyAE66(56)11(9)Fatigue20(17)2(2)Nausea14(12)1(1)Decreasedappetite12(10)0Dyspnea9(8)1(1)Diarrhea8(7)0Asthenia7(6)0Headache7(6)0Rash7(6)0Pyrexia6(5)0Vomiting6(5)1(1)Upperrespiratorytractinfection5(4)0HornL,etal.WCLC.AbstractMO18.Reprintedwithpermission.肺癌免疫治療進(jìn)展第50頁(yè)OngoingMPDL3280A(Anti-PDL-1)

ClinicalTrialsinPatientsWithNSCLCLineofTherapyPhasePD-L1SelectionComparatorSingle-agentMPDL3280A1stline;≥2ndline[1]IIYesNA1stline;≥2ndline[2]IIYesNA2ndline[3]IINoDocetaxel≥2ndline[4]IIINoChemotherapyCombinationMPDL3280AExpansion:EGFRm