紫花地丁的化學(xué)成分研究

紫花地丁的化學(xué)成分研究

1violyedoingole,vi逐漸形成zihudiget是中國的一個常見使用。從疾病的角度來看，這是一個由家庭感染的異常數(shù)量。它被用作反射性和defender動物，母性和殼干。2抗混事務(wù)四:ate,etoac,n,n-pch,etoac,n-生物,n,n,n,n,n,n,n,n,n,n-pc-3,,4.3,4.3,..和etoaph法治雙軌的雙軌道報到,和和.3.4和和....3.4和.3.4和.3.4和.3.4和和和和和和................................3.3The75%ethanolicextractofthedriedwholeplantsofViolayedoensiswaspartitionedsuccessivelywithpetroleumether,ethylacetate,saturatedaqueousn-butanolandwater.TheEtOAcandBuOHsolublefractionsweresubjectedtorepeatedcolumnchromatographytoobtainelevencompounds.Onthebasisoftheirspectraldata(ThepresentpaperrevealsthatsixcoumarinswereisolatedandidentifiedfromViolayedoensisforthefirsttime,andtheyallpossessanti-inflammatory,antibacterial,andrelievingpainactivities3母乳喂養(yǎng)3.1u3000新型網(wǎng)絡(luò)效應(yīng)MeltingpointsweredeterminedwithaXT-4micromeltingpointapparatus(uncorrected).TheNMRexperimentswerecarriedoutusingaBrukerACF-300spectrometer(300MHzfor3.2resource軟件ViolayedoensisMakinowascollectedfromHenanProvince,ChinainJune2007.TheidentificationoftheplantwasperformedbyDr.QinMin-jian.Avoucherspecimen(No.CPU/2007/Vy/1)ofthiscollectionhasbeendepositedatDepartmentofResourcesScienceofTraditionalChineseMedicinalResources,ChinaPharmaceuticalUniversity,Nanjing,China.3.3清sixfracte-eluate和elulateThedriedwholeplants(10kg)werecoarselypowdered,andextractedthreetimeswith75%EtOH.Afterthesolventremovedundervacuum,theconcentratedextractwasfurtherextractedsuccessivelywithpet.ether(30-60°C),ethylacetate,andn-butanol,toobtainethylacetateextract(354g),andn-butanolextract(126g).Theethylacetateextract(354g)wascolumnchromatographedonsilicagelbeads1.8kg(100-200mesh),andelutedgradientlywithpet.ether(60-90°C)-acetone(100:1–2:1).Theeluatewascollectedintosixfractions(A-F).PartA(pet.ether/acetone100:1)wascolumnchromatographedonsilicagel,andelutedgradientlywithpet.ether(60-90°C)-acetoneyieldingcompound10(20mg).PartB(pet.ether/acetone50:1)wascolumnchromatographedrepeatedlyonsilicagel,andelutedgradientlywithCHCl3.4rectingmots,meoh,meoh,meoh,meoh,meoh,meoh,meoh,meoh,meoh,meoh,meoh,meCompound1Paleyellowprismaticcrystals(MeOH),lightbluefluorescenceunderUV(λCompound2Paleyellowneedlecrystals(CHClCompound3Whiteneedlecrystals(MeOH),lightpinkfluorescenceunderUV(λCompound4Whitesquareplate-likecrystals(MeOH),lightpinkfluorescenceunderUV(λCompound5Whiteneedlecrystals(MeOH),lightpinkfluorescenceunderUV(λCompound6Lightyellowpowders(MeOH).Afterreactingwith5%AlClCompound7Yellowpowders.mp284-285°C;ThemolecularformulaCCompound8Lightyellowpowders(MeOH).Showingbrightflavovirenafteritreactswith5%HCompound9Yellowpowders.ShowingyellowfluorescenceunderUV(λCompound10Whiteneedlecrystals(petroleumether),mp142-143°C.Comparisonwiththesample(β-sitosterol)byTLC,indi