CST公司產(chǎn)品及市場(chǎng)分析

CST公司產(chǎn)品介紹及市場(chǎng)分析技術(shù)部——?jiǎng)?huì)杰2012-12-28CST公司簡(jiǎn)介CellSignalingTechnology,Inc(CST)是美國(guó)著名的生物公司和細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)研究的領(lǐng)導(dǎo)者，提供齊全的、特色的信號(hào)轉(zhuǎn)導(dǎo)檢測(cè)用抗體及磷酸化抗體、ELISA試劑盒、激酶等產(chǎn)品；CST總部位于波士頓，目前在全世界有400多個(gè)員工；其產(chǎn)品被認(rèn)為是“細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)研究的金標(biāo)準(zhǔn)”！應(yīng)用分類ElisaWesternBlottingImmunohistochemistryImmunofluorescenceFlowCytometrChromatinIPIn-CellWesternElisaPathScan?SandwichELISAKits（200）PathScan?SandwichELISAAntibodyPairs（70）PathScan?Multi-TargetELISAKits（6）PathScan?AntibodyArrayKits（6）SignalKine?SandwichELISAKits（8）ComplementaryELISAProducts（9）主要檢測(cè)信號(hào)通路中的信號(hào)分子的水平，試劑盒中包括

Elisa實(shí)驗(yàn)所需的全部試劑。1PathScan?SandwichELISAKits貨號(hào)

名稱規(guī)格反應(yīng)種屬價(jià)格7179SPathScan?Total4E-BP1SandwichELISAKit1Kit(96assays)H6,118.007216SPathScan?Phospho-4E-BP1(Thr37/Thr46)SandwichELISAKit1Kit(96assays)H,M,R,Mk6,118.007179S組分體積溶液顏色4E-BP1AbCoatedMicrowells96tests4E-BP1DetectionAb11mlGreenAnti-mouseIgG,HRP-linkedAntibody11mlRedTMBSubstrate#700411mlColorlessSTOPSolution#700211mlColorlessSealingTape2sheetsELISAWashBuffer(20X)25mlColorlessCellLysisBuffer(10X)#980315mlYellowishELISASampleDiluent25mlBlue7216S組分體積溶液顏色4E-BP1(T37/46)AbCoatedMicrowells96tests4E-BP1DetectionAb11mlGreenAnti-mouseIgG,HRP-linkedAntibody11mlRedTMBSubstrate#700411mlColorlessSTOPSolution#700211mlColorlessSealingTape2sheetsELISAWashBuffer(20X)25mlColorlessCellLysisBuffer(10X)#980315mlYellowishELISASampleDiluent25mlBlue2PathScan?SandwichELISAAntibodyPairs為客戶提供多項(xiàng)、經(jīng)濟(jì)的選擇貨號(hào)

名稱規(guī)格反應(yīng)種屬價(jià)格7854SPathScan?Phospho-4E-BP1(Thr37/Thr46)SandwichELISAAntibodyPair1Kit(Reagentsfor4x96wellplates)H,M,R,Mk5,832.007854S組分體積管帽顏色保存條件4E-BP1(T37/46)CaptureAb(100X)0.4mlPink4°C4E-BP1DetectionAb(100X)0.4mlBlue4°CAnti-mouseIgG,HRP-linkedAntibody0.04mlYellow-20°C是PathScan?SandwichELISAKits的組合，有5個(gè)產(chǎn)品：CellGrowthMulti-Target；InflammationMulti-Target；MAPKinaseMulti-Target；SignalingNodesMulti-Target；ApoptosisMulti-Target（CST專家努力研發(fā)中）。3PathScan?Multi-TargetSandwichELISAKits貨號(hào)

名稱規(guī)格反應(yīng)種屬價(jià)格7274SPathScan?MAPKinaseMulti-TargetSandwichELISAKit1Kit(96assays)H,M,7,712.00PathScan?MAPKinaseMulti-TargetSandwichELISAKit組分組分體積溶液顏色管帽顏色Phospho-p44/42MAPK(Thr202/Tyr204)RabbitAntibodyCoatedMicrowells16testsp44/42MAPKMouseDetectionAntibody1.8mlGreenLightPinkAnti-MouseIgGHRP-LinkedAntibody1.8mlRedLightPinkp38αMAPK(T180/Y182)MouseAbCoatedMicrowells16testsPhospho-p38αMAPK(Thr180/Tyr182)RabbitDetectionAb1.8mlGreenYellowAnti-RabbitIgGHRP-LinkedAb1.8mlRedYellowMEK1MouseAbCoatedMicrowells16testsMEK1RabbitDetectionAb1.8mlGreenWhiteAnti-RabbitIgGHRP-LinkedAb1.8mlRedWhiteMEK1MouseAbCoatedMicrowells16testsPhospho-MEK1/2(Ser217/221)RabbitDetectionAb1.8mlGreenGreenAnti-RabbitIgGHRP-LinkedAb1.8mlRedGreenSAPK/JNKMouseAntibodyCoatedMicrowells16sheetsSAPK/JNKRabbitDetectionAb1.8mlGreenGrayAnti-RabbitIgGHRP-LinkedAb1.8mlRedGraySAPK/JNKMouseAntibodyCoatedMicrowells16testsPhospho-SAPK/JNK(Thr183/Tyr185)RabbitDetectionAntibody1.8mlGreenPurpleAnti-rabbitIgGHRP-LinkedAntibody1.8mlRedPurpleTMBSubstrate11mlColorlessSTOPSolution11mlColorlessSealingTape2sheets20XWashBuffer25mlColorlessSampleDiluent25mlBlueCellLysisBuffer(10X)#980315mlYellowish4PathScan?AntibodyArrayKits

研究一個(gè)信號(hào)通路中相關(guān)蛋白的水平貨號(hào)

名稱規(guī)格反應(yīng)種屬9700SPathScan?AktSignalingAntibodyArrayKit(FluorescentReadout)1Kit(32multiplexedassays)H,M9700S組分規(guī)格管帽顏色ArraySlides-AktSignalingArray2Ea16-WellGasket2EaSealingTape2sheets20XArrayWashBuffer15mlWhiteArrayBlockingBuffer5mlRedArrayDiluentBuffer15mlBlueDyLight680?-linkedStreptavidin(10X)300μlBrownPathScan?SandwichELISALysisBuffer(1X)#701830mlClearDetectionAntibodyCocktail(10X)300μlPathScan?AktSignalingAntibodyArrayKit(FluorescentReadout)

TargetPhosphorylationSite1PositiveControlN/A2NegativeControlN/A3AktThr3084AktSer4735S6RibosomalProteinSer235/2366AMPKαThr1727PRAS40Thr2468mTORSer24819GSK-3αSer2110GSK-3βSer911p70S6KinaseThr38912p70S6KinaseThr421/Ser42413BadSer11214RSK1Ser38015PTENSer38016PDK1Ser24117Erk1/2Thr202/Tyr204184E-BP1Thr37/465SignalKine?SandwichELISAKits貨號(hào)

名稱規(guī)格8628SignalKine?HumanEGFChemiluminescentSandwichELISAKit1Kit((96assays)LowVolumeMicroplate)8667SignalKine?HumanEGFSandwichELISAKit1Kit((96assays)LowVolumeMicroplate)8669SignalKine?HumanIL-4SandwichELISAKit1Kit((96assays)LowVolumeMicroplate)7921SignalKine?HumanIL-8ChemiluminescentSandwichELISAKit1Kit((96assays)LowVolumeMicroplate)7898SignalKine?HumanIL-8SandwichELISAKit1Kit((96assays)LowVolumeMicroplate)8629SignalKine?HumanIL-2ChemiluminescentSandwichELISAKit1Kit((96assays)LowVolumeMicroplate)7289SignalKine?HumanTNF-αSandwichELISAKit1Kit((96assays)LowVolumeMicroplate)8668SignalKine?HumanTNF-αChemiluminescentSandwichELISAKit1Kit((96assays)LowVolumeMicroplate)6ComplementaryELISAProductsPathScan?ELISAControlCellExtractsPathScan?SandwichELISALysisBuffer(1X)#7018BSA#9998CellLysisBuffer(10X)#9803PhosphateBufferedSaline(PBS-20X)#9808PhosphateBufferedSalinewithTween20(PBST-20X)#9809STOPSolution#7002TMBSubstrate#7004WesternBlotting目前，CST抗體有5000種，主要是細(xì)胞信號(hào)通路相關(guān)產(chǎn)品，每種抗體一般都有多種應(yīng)用，如W、IP、IHC-P、IF、F等。貨號(hào)

名稱規(guī)格反應(yīng)種屬應(yīng)用價(jià)格4370PPhospho-p44/42MAPK(Erk1/2)(Thr202/Tyr204)(D13.14.4E)XP?RabbitmAb40ul(8westernblots)HMRHmMkMiDmZBDgPgSc(Ce)WIPIHC-PIF-ICF2,100.004370SPhospho-p44/42MAPK(Erk1/2)(Thr202/Tyr204)(D13.14.4E)XP?RabbitmAb200ul(40westernblots)HMRHmMkMiDmZBDgPgSc(Ce)WIPIHC-PIF-ICF3,836.004370LPhospho-p44/42MAPK(Erk1/2)(Thr202/Tyr204)(D13.14.4E)XP?RabbitmAb600ul(120westernblots)HMRHmMkMiDmZBDgPgSc(Ce)WIPIHC-PIF-ICF9,196.00ApplicationsKey:

W=WesternBlotting

IP=Immunoprecipitation

IHC-P=Immunohistochemistry(Paraffin)

IF-IC=Immunofluorescence(Immunocytochemistry)

F=FlowCytometry

ReactivityKey:

H=Human

M=Mouse

R=Rat

Hm=Hamster

Mk=Monkey

C=Chicken

Mi=Mink

Dm=D.melanogaster

Z=Zebrafish

B=Bovine

Dg=Dog

Pg=Pig

Sc=S.cerevisiae

Ce=C.elegansWesternBlottingλphosphatase或TPA處理293,NIH/3T3和C6細(xì)胞系，然后抽提蛋白進(jìn)行Westernblot，Phospho-p44/42MAPK(Erk1/2)(Thr202/Tyr204)(D13.14.4E)XP?RabbitmAb(#4370)(上),p44/42MAPK(Erk1/2)(137F5)RabbitmAb（#4695）

(下）U0126(#9903)(10μMfor1h)或TPA(#4174)(200nMfor10m)處理COS細(xì)胞系，然后抽提蛋白進(jìn)行Westernblot，Phospho-p44/42MAPK(Erk1/2)(Thr202/Tyr204)(D13.14.4E)XP?RabbitmAb(#4370)（左），p44/42MAPK(Erk1/2)(3A7)MousemAb(#9107)（右）Phospho-p44/42MAPK(Erk1/2)(Thr202/Tyr204)(D13.14.4E)XP?RabbitmAbImmunohistochemistry

人乳腺癌石蠟包埋切片的免疫組化，Phospho-p44/42MAPK(Erk1/2)(Thr202/Tyr204)(D13.14.4E)XP?RabbitmAb(#4370).人肺癌石蠟包埋切片的免疫組化，λphosphatase未處理（左）或處理（右），Phospho-p44/42MAPK(Erk1/2)(Thr202/Tyr204)(D13.14.4E)XP?RabbitmAb(#4370).Phospho-p44/42MAPK(Erk1/2)(Thr202/Tyr204)(D13.14.4E)XP?RabbitmAbImmunofluorescence

激光共聚焦顯微鏡觀察U0126#9903(left,10μMfor1h)或TPA#9905(right,200nMfor15min)處理的C2C12細(xì)胞系，hospho-p44/42MAPK(Erk1/2)(Thr202/Tyr204)(D13.14.4E)XP?RabbitmAb(green)，ActinfilamentshavebeenlabeledwithAlexaFluor?555phalloidin(red).Bluepseudocolor=DRAQ5?#4084(fluorescentDNAdye).Phospho-p44/42MAPK(Erk1/2)(Thr202/Tyr204)(D13.14.4E)XP?RabbitmAbFlowCytometrPhospho-p44/42MAPK(Erk1/2)(Thr202/Tyr204)(D13.14.4E)XP?RabbitmAb流式分析U0126-treated(blue)或PMA-treated(green)處理過(guò)的Jurkat細(xì)胞，抗體是Phospho-p44/42MAPK(Erk1/2)(Thr202/Tyr204)(D13.14.4E)XP?RabbitmAb.BuffersandReagents（15）SecondaryAntibodies（19）DetectionReagents（4）ProteinMarkers（2）LoadingControlAntibodies（26）ControlCellExtracts&Proteins（26）IPReagentSolutions（15）BoostDetectionReagents（2）DABSubstrate（1）ControlSlides（8）IsotypeControls（2）BlockingPeptides（4）EpitopeTagAntibodies（55）Inhibitors/Activators（44）siRNA（300）WB/IP/IHC/IF/FC輔助試劑ChromatinIP染色質(zhì)免疫沉淀法（Chromatinimmunoprecitation，ChIP）是研究體內(nèi)DNA與蛋白質(zhì)相互作用的重要工具。它可以靈敏地檢測(cè)目標(biāo)蛋白與特異DNA片段的結(jié)合情況，還可以用來(lái)研究組蛋白與基因表達(dá)的關(guān)系。該技術(shù)主要應(yīng)用于：1.組蛋白修飾酶的抗體作為“生物標(biāo)記”2.轉(zhuǎn)錄調(diào)控分析3.藥物開發(fā)研究4.有絲分裂研究5.DNA損失與凋亡分析ChromatinIP目前CST的ChIP產(chǎn)品有：ChIPValidatedAntibodiesandPrimerPairs（146）SimpleChIP?AssayKits（3）SimpleChIP?EnzymaticChromatinIPKits（2）SimpleChIP?PlusEnzymaticChromatinIPKits（2）SimpleChIP?ControlPCRPrimers（43）貨號(hào)

名稱規(guī)格反應(yīng)種屬價(jià)格8982SSimpleChIP?HumanBivalentPromoterAssayKit1Kit(10immunoprecipitations)H6,416.00ChromatinIP貨號(hào)

名稱規(guī)格反應(yīng)種屬價(jià)格8982SSimpleChIP?HumanBivalentPromoterAssayKit1Kit(10immunoprecipitations)H6,416.00試劑盒組分規(guī)格Tri-Methyl-HistoneH3(Lys4)(C42D8)RabbitmAb#9751100μlTri-Methyl-HistoneH3(Lys27)(C36B11)RabbitmAb#9733100μlSimpleChIP?HumanGAPDHExon1Primers#5516500μlSimpleChIP?HumanMYT-1Exon1Primers#4493500μlSimpleChIP?HumanGATA-6PromoterPrimers#5550500μlChromatinIPChIP輔助試劑：貨號(hào)產(chǎn)品名稱規(guī)格價(jià)格7017S6-TubeMagneticSeparationRack1units2,204.009006SChIP-GradeProteinGMagneticBeads1ml(30immunoprecipitations)2,268.009007SChIP-GradeProteinGAgaroseBeads1ml(30immunoprecipitations)1,620.00KinaseAssayKinaseAssayReagents（60）NonradioactiveKinaseAssays（3）

cAMPandcGMPEIAkits（4）貨號(hào)

名稱規(guī)格反應(yīng)種屬價(jià)格9624SPhospho-PKASubstrate(RRXS*/T*)(100G7E)RabbitmAb100ul(10westernblots)All3,662.009840SAktKinaseAssayKit40assaysH,M,R4,731.004360SCyclicGMPXPAssayKit1Kit(96assays)All3,824.00NonradioactiveKinaseAssays（3）

NonradioactiveKinaseAssayKitOverviewStep1:

加入固定化Akt抗體Step2:

加入重組融和蛋白（底物）和ATP與微珠共孵育Step3:

以WB或化學(xué)發(fā)光方法用底物特異性磷酸化抗體檢測(cè)磷酸化的底物9840S