PFI-90-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEPFI-90Cat.No.:HY-139348CASNo.:53995-62-3分?式:C??H??N?O分?量:214.22作?靶點(diǎn):HistoneDemethylase作?通路:Epigenetics儲(chǔ)存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:100mg/mL(466.81mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM4.6681mL23.3405mL46.6810mL5mM0.9336mL4.6681mL9.3362mL10mM0.4668mL2.3340mL4.6681mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:2.5mg/mL(11.67mM);Clearsolution;Needultrasonic1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE2.請(qǐng)依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(11.67mM);ClearsolutionBIOLOGICALACTIVITY?物活性PFI-90?種組蛋?去甲化酶(KDM3B)選擇抑制劑，可抑制PAX3-FOXO1的作?。PFI-90可誘導(dǎo)細(xì)胞凋亡和肌性分化，導(dǎo)致細(xì)胞死亡的增加。PFI-90具有潛在的抗腫瘤活性，來(lái)?專利WO2021101929A1。IC50&TargetIC50:812nM(RH4);3200nM(RH30);1895nM(OSA-CL);1113nM(TC-32)[1]體外研究PFI-90showsdoseresponseinRH4,RH30,OSA-CLandTC-32cells,withIC50sof812,3200,1895and1113nM,respectively[1].PFI-90(3μΜ;24hours)increasesapoptosisinRH4andSCMCcells[1].WesternBlotAnalysis[1]CellLine:RH4andSCMCcellsConcentration:3μΜIncubationTime:24hoursResult:ApoptosisincreasedinRH4andSCMCcells.REFERENCES[1].KHAN,Javed,etal.Inhibitorsofhistonedemethylases(pfi-63andpfi-90)forthetreatmentofcancerandfortheinhibitionofhistonedemethylaseincells.WO2021101929A1.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3