Ilexgenin-A-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEIlexgeninACat.No.:HY-N6255CASNo.:108524-94-3分?式:C??H??O?分?量:502.68作?靶點(diǎn):Others作?通路:Others儲(chǔ)存?式:4°C,protectfromlight*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light)溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:100mg/mL(198.93mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM1.9893mL9.9467mL19.8934mL5mM0.3979mL1.9893mL3.9787mL10mM0.1989mL0.9947mL1.9893mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month(protectfromlight)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:2.5mg/mL(4.97mM);Suspendedsolution;Needultrasonic2.請(qǐng)依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(4.97mM);Clearsolution1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEBIOLOGICALACTIVITY?物活性IlexgeninA從海南冬中提取的?種五環(huán)三萜類化合物。IlexgeninA可?于炎癥和癌癥研究[1]。體外研究IlexgeninA(0~100μM;24hours;HT29andHCT116cells)effectivelydecreasescellviabilityinHT29andHCT116cells[1].IlexgeninA(12.5~50μM;24hours;HT29cells)downregulatestheexpressionofSREBP-1,inhibitsthetranslocationofSREBP-1tothenucleusanddecreasesthecontentofTG[1].IlexgeninA(12.5~50μM;HT29andHCT116cells)inducescellcyclearrestatG1phaseincoloncancercells[1].IlexgeninA(12.5~50μM;24hours;HCT116cells)modulateslipidmetabolism.IlexgeninA(50μM;24hours;HT29andHCT116cells)regulatesSREBP-1byinhibitingHIF1α[1].IlexgeninAinhibitsfattyacidssynthesis[1].CellViabilityAssay[1]CellLine:HT29andHCT116cellsConcentration:0~100μMIncubationTime:24hoursResult:EffectivelydecreasedcellviabilityinHT29andHCT116cells.WesternBlotAnalysis[1]CellLine:HT29cellsConcentration:12.5~50μMIncubationTime:24hoursResult:DownregulatedtheexpressionofSREBP-1.RT-PCR[1]CellLine:HT29cellsConcentration:12.5~50μMIncubationTime:24hoursResult:DecreasedthecontentofTG.Immunofluorescence[1]CellLine:HT29cells2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEConcentration:12.5~50μMIncubationTime:24hoursResult:InhibitedthetranslocationofSREBP-1tothenucleus.體內(nèi)研究IlexgeninA(20mg/kg)amelioratestheAOM/DSSinducedcarcinogenesis.IlexgeninAcanregulatelipidmetabolism.IlexgeninAdecreasestheexpressionofSREBP-1andHIF1αinAOM/DSSmice[1].REFERENCES[1].ZhangL,etal.IlexgeninApreventsearlycoloniccarcinogenesisandreprogramedlipidmetabolismthroughHIF1α/SREBP-1.Phytomedicine.2019;63:153011.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedica