講義相關(guān)周匯報(bào)

1、GroupDiscussionPart 1 Mapping of the QTL conferring resistance to leaf and panicle blast in Japonica landrace JiangnanwanIsolatesOriginJNWSYN leaf blastpanicle blast leaf blastpanicle blast191(ZB13)JSHRMRHSHS97-2(ZC15)JSHRMRHSHS35-1(ZD1)JSHRHRHSHS55-1(ZE3)JSHRMRHSHS42-2(ZF3)JSSMSHSHS113(ZG1)JSRSHSHS

2、Hoku 1(ZG1)JapanHRMRHSHSTable 1 the resistance of parents after infect the M.oryzae isolatesSYNJNWSYNJNWAB圖1 A 江南晚和蘇御糯稻瘟病抗感表型；B 接種北1后的病級(jí)類型Fig.1 A Phenotypes of panicle and leaf blast in JNW (left) and SYN(right). B The disease scores after infected hoku1Diseased grains(%)Score of leaf blast severity

3、* P=0.01* P=0.05*SYNJNWSYNJNW2040608012345Results圖 2 （A-C) 蘇御糯與江南晚雜交不同后代中苗瘟抗性級(jí)別分布Figure 2 (A-C) Frenquency distribution of leaf blast among different progeny lines derived from a cross between JNW and SYN. ACBNumber of linesScore of leaf blast severityScore of leaf blast severityScore of leaf blast

4、severityNumber of linesNumber of linesF2:6 NJAU F2:6 JAASF2:7 JAASResults圖 3 蘇御糯與江南晚雜交不同后代中苗瘟抗性級(jí)別分布Fig.3 Frenquency distribution of panicle blast among different progeny lines derived from a cross between JNW and SYNResultsTraitQTLChrPositionLeftMarker RightMarkerLOD PVE(%)AddLBRq.lb 4.1(2014JS)4143

5、RM6089RM38143.67485.61520.3095q.lb 11.1(2014JS)11217RM27273BS3314.430524.57870.6499q.lb 11.3(2015JS)11217RM27273BS3320.811237.19140.9699q.lb 11.3(2015JS)11217RM27273BS336.244212.27140.2034PBRq.pb 7.1(2014JS)778RM3186RM3464.86774.8850-0.0714q.pb 11.1(2014JS)11237BS39BS3329.994148.22330.2261q.pb 4.1(2

6、015HN)4110RM6089RM38144.03183.27840.0577q.pb 5.1(2015HN)59RM1182RM2332.84464.37050.0663q.pb 11.2(2015HN)11207RM7277RM271223.94393.0996-0.0556q.pb 11.3(2015HN)11235RM27273BS3949.199870.18320.2678q.pb 4.1(2015JS)40RM16335RM82133.61743.70950.0627q.pb11.4(2015JS)11228RM27150RM272736.14237.05110.0865q.pb

7、11.5(2015JS)11237BS39BS3322.590831.87900.1884Analyzed by QTL IciMapping V4.0Table 2 All QTLs detected for disease gradeResultsFig.4 (A-C) the correlations between panicle and leaf blast resistance severity；X: The lesion scores panicle blast， Y: The lesion scores leaf blast CABResultsRM7277RM27122RM2

8、7273RM27129BS39RM27150RM27381Chr111.5cM1.006 5.9535.973 8.341 7-4-7R6430-1-3R1-5-611-1-10SSQpb1(250kb）?100kbRM27150RM27381BS33JiangnanwanSuyunuoJ/S927100520.5147.5(2)(0)(0)(2)(2)Figure4 A Genetic and physical maps of the region of the qpb1 locus.RM27273RM27381BC2F1+BC1F1260株結(jié)合帶型挑選單株接種W28W26BS71(1)(0

9、)(0)150132143Results精細(xì)定位Pb-jnw1等待接種表型結(jié)果。驗(yàn)證Pb-jnw1是否同時(shí)影響苗瘟抗性挑選攜帶Pb-jnw1純合位點(diǎn)的單株苗期接種，觀察抗感情況。構(gòu)建育種中間材料將江南晚與南粳46、南粳9108及鎮(zhèn)稻88雜交得到的F1，結(jié)合分子標(biāo)記挑選攜帶Pb-jnw1位點(diǎn)的后代個(gè)體分別南粳46、南粳9108、鎮(zhèn)稻88回交構(gòu)建育種中間材料。討論穗瘟接種及統(tǒng)計(jì)發(fā)病方法的優(yōu)化家系不同的生育期一定要設(shè)置多個(gè)抗感對(duì)照；百分比統(tǒng)計(jì)方法的依據(jù)？抗感的區(qū)分？為什么出現(xiàn)偏分布情況？BPart 2 Graduation Dissertation第一章 文獻(xiàn)綜述1 數(shù)量性狀的研究1.1 數(shù)量性狀基因座的定位1.1.1 連鎖定位1.1.2 關(guān)聯(lián)定位1.2 QTL克隆2 水稻抗稻瘟病性的研究進(jìn)展 2.1 水稻稻瘟病的侵染途徑和發(fā)病類型2.2 水稻稻瘟病發(fā)病影響因素和接種鑒定2.3 水稻抗稻瘟病類型和遺傳研究2.4 水稻抗稻瘟病基因的定位2.5 水稻抗稻瘟病基因的克隆2.6 水稻抗稻瘟病基因的類型2.7 抗稻瘟病育種應(yīng)用第二章 水稻