食品化學進展及水產(chǎn)化學

1、會計學1食品化學進展及水產(chǎn)化學食品化學進展及水產(chǎn)化學研究內(nèi)容研究內(nèi)容:1引起蝦類黑變真正原因是什么？引起蝦類黑變真正原因是什么？MelaninDOPA chromeDOPA quinoneDOPATyrosine5, 6-dihydroxyindole (DHI)Phenoloxidase (PO) Prophenoloxidase(ProPO)Phenoloxidase(PO) Beta-1,3 glucan, LPS, Peptidoglycan p-APMSF 1,3葡聚糖，葡聚糖， 脂多糖，肽聚糖脂多糖，肽聚糖ProPO Systemprophenoloxidase activatin

2、g factor多酚氧化酶原的多酚氧化酶原的cDNA序列及由序列及由cDNA序列推導出的氨基酸序列序列推導出的氨基酸序列Proteinsidentity (%)Pacifastacus leniusculus prophenoloxidase57Bombyx moriprophenoloxidase35Drosophila melanogaster prophenoloxidase38Homo sapiens tyrosinase15Oryzias latipes tyrosinase13Neurospora crassa tyrosinase14Sinorhizobium meliloti

3、tyrosinase11Panulirus interruptushemocyanin subunit A29Penaeus vannamei hemocyanin30Eurypelma californicum hemocyanin subunit a38不同來源的多酚氧化酶原氨基酸序列的同源性比較不同來源的多酚氧化酶原氨基酸序列的同源性比較 A and C: ActivatedHemocyanin B and D: Native Hemocyanin 在蝦的表皮會發(fā)生明顯的黑變在蝦的表皮會發(fā)生明顯的黑變血藍蛋白存在于蝦類的表皮血藍蛋白存在于蝦類的表皮? DorsalVentral ABCD

4、EpicuticleExocuticleEndocuticle100nmMelaninDOPA chromeDOPA quinoneDOPATyrosine5, 6-dihydroxyindole (DHI)Hemocyanin ？5,6-Dihydroxyindole (DHI)MelaninDOPA chromeDOPA quinoneDOPATyrosine5, 6-dihydroxyindole (DHI)Hemocyanin ？MelaninDOPA chromeDOPA quinoneDOPATyrosine5, 6-dihydroxyindole (DHI)Hemocyanin ？Action time 5 10 25 15 30 20 50 35 60 45 40 55 60 Min. A：HemocyaninMCF B：Hemocyanin C：MCFABCMCF活性在對蝦凍結貯藏過程中的變化活性在對蝦凍結貯藏過程中的變化 MelaninDOPA chromeDOPA quinoneDOPATyrosi