注余不茍同Watson諸人以操作之對(duì)象來劃分章節(jié)也竊以為

1、注：余不茍同Watson諸人以操作之對(duì)象來劃分章節(jié)也。竊以為，參考分子操作的具體步驟可以更好地理解分子生物學(xué)技術(shù)本身哉。余雖淺陋，然不愿輕棄己意遂有此PPT。 fig 1 principle of Ion exchange chromatographyFig 2 Principle of gel filtration chromatographyFig 3 Principle of Affinity ChromatographyLinear DNA molecules migrate through the gel toward the positive pole with different

2、 rates according to different sizelPulsed-field gel electrophoresis forlong DNAs (up to severalMb in length).Recognition sequences and cut sites of various endonucleasesThe absence of 3-hydroxyl lead to the inefficiency of the nucleophilic attack on the next incoming substrate molecule DNA molecules

3、 (radioactively labeled at 5 termini) are subjected to 4 regiments to be broken preferentially at Gs, Cs, Ts, As, separately.4 systems with dNTP+ ddGTP, dNTP+ ddATP d NTP+ ddCTP, d NTP+ ddTTP separately.And even ,we can “read” the sequencing gel to get the sequence of the DNAWith the method of fluor

4、escent chain-terminating nucleotides, we can carry out high throughput sequenceread containing identical sequences are assumed to overlap and are joined to form larger contigs .And paired-end reading leads to form scaffold . Schematic model Space-filling modelThe N-terminal residue is labeled and ca

5、n be removed without hydrolyzing therest of the peptide. Thus, in each round, one residue is identified, and thatresidue represents the next one in the sequence of the peptide. Material travels through the instrument in a manner that is sensitive to its mass/charge ratio. It is a method with great accuracy.The polypeptide exit tunnel in 50s subunits.High resolution structure of the RuvA-DNA complex and schematic model of the RuvAB complex bound to Holliday junction DNA.An example :Comparison of a 34kb region of th