美國FDA批準(zhǔn)新腫瘤標(biāo)志物-10-25-09

1、PHENOMICS DISCOVERY PLATFORM 蛋白指紋圖譜蛋白指紋圖譜蛋白指紋圖譜儀蛋白指紋圖譜儀10年年1999-2009年年蛋白質(zhì)組學(xué)研究與應(yīng)用基因組學(xué)研究基因組學(xué)研究IDID與定性與定性TerminalNode 1Class = 0ClassCases%06 100.0100.0N = 6TerminalNode 2Class = 0ClassCases%02 100.0100.0N = 2TerminalNode 3Class = 1ClassCases%000.015 100.0N = 5Node 4Class = 1M7466_57 = 4.674ClassCases%

2、0228.61571.4N = 7Node 3Class = 0M9134_31 = 3.006ClassCases%0861.51538.5N = 13TerminalNode 4Class = 1ClassCases%015.311894.7N = 19Node 2Class = 1M6430_42 = 21.569ClassCases%0928.112371.9N = 32TerminalNode 5Class = 0ClassCases%016 100.0100.0N = 16TerminalNode 6Class = 1ClassCases%000.014 100.0N = 4Ter

3、minalNode 7Class = 0ClassCases%02 100.0100.0N = 2Node 6Class = 1M3816_96 = 12.753ClassCases%0233.31466.7N = 6Node 5Class = 0M7562_65 = 5.830ClassCases%01881.81418.2N = 22Node 1Class = 0M3955_97 = 11.164ClassCases%02750.012750.0N = 54分離純化分離純化臨床檢驗(yàn)臨床檢驗(yàn)Use of proteomic patterns in serum to identify ovar

4、ian cancer. Lancet. 2002 Feb 16;359: 572-7 標(biāo)準(zhǔn)標(biāo)準(zhǔn)1Zhang, et al, Three Biomarkers Identified from Serum Proteomic Analysis for the Detection of Early Stage Ovarian Cancer, Cancer Research 2004; 64. 標(biāo)準(zhǔn)標(biāo)準(zhǔn)2 Common ProteinsSpecific FingerprintsDisease ProcessesPancreatic CancerOvarian CancerDiabetesnvhsgst

5、ffkyylqgakipkpeasfsprmnfrpgvlssrqlglpgppdvpdhaayhpfrsrqlglgppdvpdhaayhpfrDisease specific fingerprintsDisease specific tissue reactionCirculating proteinsOvarian cancerBreast cancerPancreatic cancerBenign diseaseFDA Sept. 11, 2009NormalAcute Clinical RejectionStable Transplant01000020000300004000001

6、0000200003000040000 肺癌5335 正常-2 502 55 07 501 0 0 0 02 0 0 0 03 0 0 0 04 0 0 0 0-2 502 55 07 501 0 0 0 02 0 0 0 03 0 0 0 04 0 0 0 0肺癌正常肺癌正常a-1 antitrypsin(抗胰島素)44324 (60000)pI 5.371.8 mg/mla-1 antichymotrypsin(抗凝乳胰島素)45265.8 (62000)pI 5.32a-2 antiplasmin50450.9pI 5.87Antithrombin III49039 (64000)pI

7、5.95Ceruloplasmin120085pI 5.4Clusterin (apo-J)50062.6 (40000)pI 5.89Clusterin A chain25883pI 5.66Complement C3 a chain113028pI 5.55Complement C3 b chain71332.7pI 6.82Complement C3184967.4pI 6.0Complement C4 g chain33073.6pI 6.37Complement factor D23800Connective tissue activating peptide III (CTAP)9

8、291.7pI 7.84C-reactive protein(C反應(yīng)蛋白)23047pI 5.282-100 ng/mlFerritin(鐵蛋白)70 ng/mla-1B glycoprotein51940.7 (72000)pI 5.65Haptoglobulin a-1 chain9192.2pI 5.23Haptoglobulin a-2 chain15945.8pI 5.57Haptoglobulin b chain27265 (42000)pI 6.32Haptoglobulin 1364302 mg/mlHemoglobin a chain15126.4pI 8.73Hemoglo

9、bin b chain15867.2pI 6.81Hemopexin49295.4 (73000)pI 6.43Histidine-rich glycoprotein57660 (65000)pI 7.03a-2-HS glycoprotein chain A30221.9 (55000)pI 4.53IgA3 mg/mlIgG12 mg/mlIgM1.5 mg/mlKininogen light chain28235.7pI 6.36Leucine-rich a-2 glycoprotein (LRG)34346.4pI 5.66a-2 macroglobulin160797pI 5.952

10、.2 mg/mla-1 microglobulin20846.7pI 6.13b-2 microglobulin20 ng/mlMyoglobin(肌血球素)25-45 ng/mlOrosomucoid acid ( a-1 acid glycoprotein)21560 (45000)pI 5.0Paraoxonase39618 (45000)pI 5.08Plasminogen88300pI 7.08Serum amyloid P component23258.5pI 6.12Serum albumin66472pI 5.6727-39 mg/ml plasmaTransferrin(鐵傳

11、遞蛋白)75181.4 (78000) pI 6.73.5 mg/mlTransferrin receptor(鐵傳遞蛋白受體)2 mg/mlTransthyretin13761.4 (15000, 30000) pI 5.35Troponin I (cardiac)(肌鈣蛋白I-強(qiáng)心劑)0.5 ng/mlVitronectin V10 subunit9266.4pI 5.08Zinc a-2 glycoprotein32145 (40000)pI 5.58Lipoproteins:HDL1.7 mmole/ml plasmaLDL4.3 mmole/ml plasmaVLDL11100 mg/ml plasmaVLDL21030 mg/mlplasmaIDL1256 mg/mlplasmaApolipoprotein A-I (阿樸脂蛋白)28078.6 pI 5.271-1.5 mg/mlplasmaApolipoprotein A-II 8707.9pI 5.05Apolipoprotein A-IV 43374.5pI 5.2Apolipoprotein B10070 mg/m