生物化學(xué)試題2006秋季卷

生物化學(xué)I（2006年秋季學(xué)期A卷 IV《中山大學(xué)授予學(xué) “ ”簡(jiǎn)答題（共25小題，每小題4分50分以50分計(jì)入總分哪些標(biāo)準(zhǔn)氨基酸含兩個(gè)手性碳原子？寫出它們的Fisher為什么SDS變性電泳可以估算蛋白質(zhì)的分子量Massspectroscopy在生物大分子研究中有什么應(yīng)用-Keratin、Collagen、Silkfibroin的二級(jí)結(jié)構(gòu)和氨基酸組成有何特點(diǎn)invivoHemoglobin與O2的親和力增加圖示抗體的一般結(jié)構(gòu)（包括結(jié)構(gòu)域及可能的二硫鍵寫出蛋白質(zhì)氨基酸的基本步驟。什么是酶催化的過(guò)渡態(tài)理論？列出你所知道的支持酶的可逆抑制劑有幾類？它們的抑制動(dòng)力學(xué)各有何特點(diǎn)細(xì)胞膜表面的Glycoprotein和GlycolipidDNA膜脂的分類及其結(jié)構(gòu)特點(diǎn)圖示secondaryactivetransport，uniport，symport，antiport.Acetylcholinereceptor通道是如何控制開關(guān)的K＋離子通道是如何對(duì)通過(guò)的離子進(jìn)行選擇的膜電位是怎樣控制Na＋通道開關(guān)的（可圖示并說(shuō)明Ca2+泵和Na+-K+泵的異同點(diǎn)。Youhaveacrudelysatesamplecontainingamixtureofsixproteins(1,2,3,4,5,andβ-galactosidase).Somecharacteristicsoftheseproteinsareshowninthetablebelow.Giveanavailableprocedureofpurifyingβ-galactosidase.(10pts)ConcentrationofammoniumrequiredforWeightpoint12345- Youareworkingonacompoundasapotentialdrugthatinhibitsanoverproducedenzymewhoseuncontrolledactivityleadstouncontrolledcellproliferation(cancer).Asteady-statekineticysisoftheenzyme-catalyzedreactionwascarriedout,withvelocitiesmeasuredasafunctionoftheconcentrationofthesubstrateintheabsenceandinthepresenceof10nMinhibitor.Theresultsofthekineticsexperimentareplottedforyoubelow.Pleaseanswerthefollowingquestions.(10pts)Whattypeofinhibitoristhiscompound(beasspecificasyoucan)?Showthediagramoftheinhibitor’smechanism(byEdenotingenzyme,Sdenotingsubstrate,Idenotinginhibitor;forexample:E+S ESE+P).CalculatetheKm,KcatintheabsenceofinhibitorandKmapp,Kappinthepresenceofinhibitor(appdenotingapparent.Thetotalconcentrationofenzymeis2nM).Calculatetheinhibitorconstant Lysozymedestroysthecellwallofbacteriabycatalyzingthehydrolysisof1,4-β-linkagesbetweenN-acetylmuramicacid(NAM)andN-acetyl-D-glucosamine(NAG)residuesinapeptidoglycan(figureA).FigureBshowsthebindingofthepeptidoglycantotheactivesiteoflysozyme,adeepcleftacrosspartoftheglobularproteinsurface.Sixhexosesofthesubstratefitintothiscleft.FigureCshowsthehydrolyticprocessFigureFigureFigureFigureWhatcanyoutalkaboutthefigureBinaccordwiththeenzymaticWhatcanyoutalkaboutthefigureCinaccordwiththeenzymaticCalculatetheratiooftheenzymemoleculeswhichreallyworkatpH3.8,5.2,6.6toallenzymemolecules,andexinfigureDaccordingtoyourcalculation(pH=pKaFigureFigure+lg([A-]/[HA])；pKaGlu=5.9,pKaAsp=FigureFigure（注意：請(qǐng)將第V,VI兩題答案寫在單獨(dú)的答題紙上，否者，自負(fù)前面已經(jīng)給出，可以采用“參見……”的方式，以節(jié)省答題時(shí)間）（10分