Sophoricoside - Flavonoid - 生命科學(xué)試劑 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemESophoricosideCat. No.: HY-N0423CAS No.: 152-95-4分式: CHO分量: 432.38作靶點(diǎn): Others作通路: Others儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 150 mg/mL (346.92 mM)* means soluble, but saturation u

2、nknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 2.3128 mL 11.5639 mL 23.1278 mL5 mM 0.4626 mL 2.3128 mL 4.6256 mL10 mM 0.2313 mL 1.1564 mL 2.3128 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Sophoricoside種從槐花中分離得到的異 酮糖苷，具有抗炎，抗癌和免疫抑制作。體外研究Sophoricoside is an isoflavo

3、ne glycoside isolated from Sophora japonica and has anti-inflammatory, anti-cancer and immunosuppressive effects. The results show that Sophoricoside (50 M) significantly inhibits thePMACI-induced histamine release. The inhibition rate reaches up to 30.24%. The maximal rates of TNF-,IL-8 and IL-6 in

4、hibition by Sophoricoside (50 M) are approximately 31.42%, 43.43% and 34.24%,1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemErespectively. The rates of the levels of Rel/p65 inhibition in nuclear by Sophoricoside (50 M) isapproximately 50.14%. Results show that the enhanced caspase-1 activity induc

5、ed by PMACI is significantlyreduced by Sophoricoside in a dose-dependent manner 1.體內(nèi)研究 When the Sophoricoside is orally administered 1 h before compound 48/80 injections, the scratchingbehaviors is reduced. The inhibition rate of Sophoricoside (2 mg/kg) is approximately 41.21%. Orallyadministered So

6、phoricoside inhibits the scratching behaviors by 47.31%. When mice are treated for 2 weekswith Sophoricoside, the atopic dermatitis is recovered to a significant extent 1.PROTOCOLKinase Assay 1 Cells are incubated with the Sophoricoside for 1 h, and then incubated with PMACI for 2 h. The reaction is

7、stopped by cooling the tubes in ice. The cells are separated from the released histamine by centrifugation at400g for 5 min at 4C. The histamine levels are measured by ELISA using a histamine assay kit accordingto the manufacturers directions. Duplicate aliquots of supernatant are measured for each

8、sample 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Cell Assay 1 To test the cell viability by each concentration of Sophoricoside, the MTT colorimetric assay is performed.Briefly, HMC-1 cells (3105 cells/well) are incubated with Sophoricoside (1 t

9、o 50 M) for 12 h. After theaddition of MTT solution, the cells are incubated at 37C for 4 h. The crystallized MTT (formazan) isdissolved in dimethyl sulfoxide and the absorbance is measured at 540 nm 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Ani

10、mal BALB/c mice (5 weeks, 19 to 20 g) are used in this study. The dorsal skin of the BALB/c mice (n=6) is shavedAdministration 1 and treated with a depilatory prior to the experiment. The mice are sensitized with 100 L of 0.15% DNCB inacetone-olive oil (3:1) applied to the dorsal skin twice per week

11、 for 5 weeks. Control mice receive vehicle(acetone/olive oil=3:1). After 3 weeks, Sophoricoside (2 mg/kg) is orally administered 2 weeks until the end ofthe experiment 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Kim SJ, et al. The ameliorative effect of sophoricoside on mast cell-mediated allergic inflammation in vivo and in vitro. Molecules. 2013May 22;18(5):6113-27.McePdfHeightCaution: Product h