Fosphenytoin-disodium-DataSheet-生命科學試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEFosphenytoin disodiumCat. No.: HY-B1657ACAS No.: 92134-98-0分式: CHNNaOP分量: 406.24作靶點: Others作通路: Others儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 H2O : 100 mg/mL (246.16 mM)* means soluble, bu

2、t saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 2.4616 mL 12.3080 mL 24.6160 mL5 mM 0.4923 mL 2.4616 mL 4.9232 mL10 mM 0.2462 mL 1.2308 mL 2.4616 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Fosphenytoin sodium是phenytoin 的前提藥物，具有抗驚厥等功效。體內(nèi)研究Fosphenytoin

3、 is an effective neuroprotectant against ischemia-induced damage. In fosphenytoin (30 mg/kg,i.m.)-treated rat 5 min after ischemia episode, hippocampal CA1 pyramidal neurons remain at near controllevel (13.90 +/- 0.92), however, GFAP staining iss not significantly changed 1. In fosphenytoin (84 mg/k

4、g)-treated rat, the relative bioavailability of fosphenytoin is 83%. In fully kindled female Wistar rats, fosphenytoin1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEdose-dependently increases the focal seizure (afterdischarge) threshold. Seizure severity and duration atthreshold are reduced only a

5、fter the highest does of fosphenytoin tested (84 mg/kg) 2.PROTOCOLAnimal A total of four groups of rats, including normal (n=2), sham operated (n=2), ischemia with saline-treatedAdministration 1 (n=2), and ischemia with fosphenytoin-treated (n=2), are studied. Postischemic rats in saline-treated and

6、fosphenytoin-treated groups receive a single i.m. injection of saline or fosphenytoin (30 mg/kg), respectively,in the right hind limb 5 minutes after resuscitation. Sham-operated animals are treated similarly except forchest compression. All rats are killed on the 7th postischemic day by decapitatio

7、n. Brains are immediatelyremoved, bisected longitudinally, and immersed in 4% buffered neutral formaldehyde containing 0.25%glutaraldehyde for a minimum of 2 days at 4C. Portions of the brain containing the dorsal hippocampus arecoronally sectioned with a vibratome at 40 m. With the aid of a dissect

8、ing microscope, rectangular blocks ofabout 1 mm2 in size encompassing the mid-CA1 region from sections that approximate Bregma -3.6 aredissected, postfixed in 2% osmium tetroxide, and dehydrated in ascending concentrations of ethanol beforebeing embedded in Araldite 502. Sections of polymerized bloc

9、ks 1 m thick are cut and toluidine blue stainedfor light microscopic examination.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Chan SA, et al. Fosphenytoin reduces hippocampal neuronal damage in rat following transient global ischemia. Acta Neurochir (Wien).1998;140(2):175-80.2. Loscher W, et al. Anticonvulsant effect of fosphenytoin in amygdala-kindled rats: comparison with phenytoin. Epilepsy Res. 1998Mar;30(1):69-76.McePdfHeightCaution: Product has not been fully validated