倍氯米松作用機(jī)制 - Medchemexpress - MCE中國

1、Product Data SheetBeclometasoneCat. No.: HY-B1540CAS No.: 4419-39-0分式: CHClO分量: 408.92作靶點(diǎn): Glucocorticoid Receptor作通路: GPCR/G Protein儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 32 mg/mL (78.25 mM)* means soluble, but saturation unknown.SolventMass1 mg 5 mg

2、10 mgConcentration制備儲備液1 mM 2.4455 mL 12.2273 mL 24.4547 mL5 mM 0.4891 mL 2.4455 mL 4.8909 mL10 mM 0.2445 mL 1.2227 mL 2.4455 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存式和期限：-80C, 6 months; -20C, 1 month。-80C 儲存時，請?jiān)?6 個內(nèi)使，-20C 儲存時，請?jiān)?1 個內(nèi)使。體內(nèi)實(shí)驗(yàn)請根據(jù)您的實(shí)驗(yàn)動物和給藥式選擇適當(dāng)?shù)娜芙獍?。以下溶解案都請先按?In V

3、itro 式配制澄清的儲備液，再依次添加助溶劑：為保證實(shí)驗(yàn)結(jié)果的可靠性，澄 的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使； 以下溶劑前顯的百分 指該溶劑在您配制終溶液中的體積占；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的式助溶1. 請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.25 mg/mL (5.50 mM); Clear solution此案可獲得 2.25 mg/mL (5.50 mM，飽和度未知) 的澄清溶液。以 1 mL 作液為例，取 100 L 22

4、.5 mg/mL 的澄 DMSO 儲備液加到 400 L PEG300 中，混合均勻；向上述體系中加50 L Tween-80，混合均勻；然后繼續(xù)加 450 L 理鹽定容 1 mL。2. 請依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.25 mg/mL (5.50 mM); Clear solutionPage 1 of 2 www.MedChemE此案可獲得 2.25 mg/mL (5.50 mM，飽和度未知) 的澄清溶液。以 1 mL 作液為例，取 100 L 22.5 mg/mL 的澄 DMSO 儲備液加到 900

5、L 20% 的 SBE-CD 理鹽溶液中，混合均勻。3. 請依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 2.25 mg/mL (5.50 mM); Clear solution此案可獲得 2.25 mg/mL (5.50 mM，飽和度未知) 的澄 溶液，此案不適于實(shí)驗(yàn)周 期在半個以上的實(shí)驗(yàn)。以 1 mL 作液為例，取 100 L 22.5 mg/mL 的澄 DMSO 儲備液加到 900 L 油中，混合均勻。BIOLOGICAL ACTIVITY物活性 Beclometasone (Beclomethasone)種糖質(zhì)激素受體 (glucocortico

6、id receptor) 激動劑。IC & Target Glucocorticoid Receptor1體外研究 An inhibition of the normal physiological neutrophil migration and of neutrophil chemotaxis directed by wounding-induced inflammation is detected at 4 h after administration of 25 M Beclomethasone. Tumour cell invasion andmicrometastasis is a

7、lso reduced in embryos incubated in 25 M Beclomethasone 4 h before implantation. In addition,the lysyl oxidase inhibitor -aminoproprionitrile (APN) largely reduces fibrillar collagen and enhances the CHT-TFtransmigration of neutrophils, leading to a significant increase of tumour cell invasion and s

8、ubsequent formation ofmicrometastases. Notably, APN inhibits neutrophil chemotaxis induced by inflammation, indicating that the increaseof tumour cell invasion in APN-treated embryos is correlated with enhanced non-pathological neutrophil migration,but not with inflammation1.PROTOCOLCell Assay 1 The

9、 transgenic lines Tg(fli1:GFP) and Tg(mpx:GFP) are used in this study. 0.2 mM N-phenylthiourea (PTU) is applied toprevent pigment formation from 1 day post-fertilization (dpf). For Pu.1 knockdown, Pu.1 MO (1 mM for partialknockdown and 2 mM for complete knockdown is injected into the yolk at the one

10、-cell stage. For pharmacologicalinhibition, the VEGFR tyrosine kinase inhibitors KRN633 (0.1-1 M) or Sunitinib (0.1-1 M), Beclomethasone (25 M)and -amino-proprionitrile (APN, 500 M) are applied directly to the egg water and refreshed every 2 days. Forpharmacological inhibition, Beclomethasone is app

11、lied to the embryos 4 h before implantation and KRN633, Sunitiniband APN are applied 4-6 h post-implantation. For each cell line or condition, data are representative of threeindependent experiments, with 30 embryos/group. Experiments are discarded when the survival rate of the controlgroup is 90%1.

12、MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) Pharm Res. 2017 Dec;34(12):2454-2465.See more customer validations on HYPERLINK www.MedChemE www.MedChemEREFERENCESPage 2 of 3 www.MedChemE1. He S, et al. Neutrophil-mediated experimental metastasis is enhanced by VEGFR inhibition in a zebrafish xenograft model. J Pathol. 2012 Aug;227(4):431-45.McePdfHeightCaution: Product has not