限制性內(nèi)切酶保護堿基表(精品文檔)_共5頁

1、PCR設(shè)計引物時酶切位點的保護堿基表切割率 %酶寡核苷酸序列2 hr20 hrGGTCGAC C00Acc ICGGTCGAC CG00CCGGTCGAC CGG00CACATGT G00Afl IIICCACATGT GG9090CCCACATGT GGG9090GGCGCGCC9090Asc IAGGCGCGCC T9090TT GGCGCGCC AA9090CCCCGGG G5090Ava ICCCCCGGG GG9090TCCCCCGGG GGA9090CGGATCC G1025BamH ICGGGATCC CG9090CGCGGATCC GCG9090CAGATCT G00Bgl I

2、IGAAGATCT TC7590GGA AGATCT TCC2590GGCGCGC C00BssH IIAG GCGCGC CT00TTG GCGCGC CAA5090BstE IIGGGT(A/T)ACC C010AACTGCAGAACCAATGCATTGG00BstX IAAAACTGCAGCCAATGCATTGG AA2550CTGCAGAA CCAATGCATTGG ATGCAT2590CATCGAT G00Cla IGATCGAT C00CCATCGAT GG9090CCCATCGAT GGG5050GGAATTC C9090EcoR ICGGAATTC CG9090CCGGAATT

3、C CGG9090Hae IIIHind IIIKpn IMlu INco INde INhe INot INsi IPac IPme IGGGGCC CC9090AGC GGCC GCT9090TTGC GGCC GCAA9090CAAGCTT G00CCAAGCTT GG00CCCAAGCTT GGG1075GGGTACC C00GGGGTACC CC9090CGGGGTACC CCG9090GACGCGT C002550CGACGCGT CGCCCATGG G00CATG CCATGG CATG5075CCATATG G00CCCATATG GG00CGCCATATG GCG00GGGT

4、TT CATATG AAACCC00GGAATTC CATATG GAATTCC7590GGGAATTC CATATG GAATTCCC7590GGCTAGC C00CGGCTAGC CG1025CTA GCTAGC TAG1050TT GCGGCCGC AA00ATTT GCGGCCGC TTTA1010AAATAT GCGGCCGC TATAAA1010ATAAGAATGCGGCCGC TAAACTAT2590AAGGAAAAAAGCGGCCGC AAAAGGAA2590AATGCATGCAT GCA10909090CCA ATGCAT TGGTTCTGCAGTTTTAATTAA00GTT

5、AATTAAC025CCTTAATTAAGG090GTTTAAAC00GGTTTAAACC025GGGTTTAAACCC050AGCTTT GTTTAAAC GGCGCGCCGG7590Pst IPvu ISac ISac IISal ISca ISma ISpe ISph IStu IXba IGCTGCAG C00TGCA CTGCAG TGCA1010AA CTGCAG AACCAATGCATTGG9090AAAA CTGCAG CCAATGCATTGGAA9090CTGCAG AACCAATGCATTGGATGCAT00CCGATCG G00AT CGATCG AT1025TCG CG

6、ATCG CGA010CGAGCTC G1010GCCGCGG C00TCCCCGCGG GGA5090GTCGAC GTCAAAAGGCCATAGCGGCCG00C1050GCGTCGAC GTCTTGGCCATAGCGGCCGC1075GGACGC GTCGAC GTCGGCCATAGCGGCCGCGGAAGAGTACT C10257575AAA AGTACT TTTCCCGGG010CCCCGGG G010CCCCCGGG GG1050TCCCCCGGG GGA9090GACTAGT C1090GGACTAGT CC1090CGGACTAGT CCG050CTAG ACTAGT CTAG

7、050GGCATGC C00CAT GCATGC ATG025ACAT GCATGC ATGT1050A AGGCCT T9090GA AGGCCT TC9090AAA AGGCCT TTT9090CTCTAGA G00GCTCTAGA GC9090TGCTCTAGA GCA7590Xho IXma ICTAG TCTAGA CTAG7590CCTCGAG G00CCCTCGAG GG1025CCGCTCGAG CGG1075CCCCGGG G00CCCCCGGG GG2575CCCCCCGGG GGG5090TCCC CCCGGG GGGA9090ApaI（類型： Type II restr

8、iction enzyme）識別序列：5GGGCCC3BamHI （類型： Type II restriction enzyme）識別序列：5 GGATCC3BglII（類型： Type II restriction enzyme）識別序列：5 AGATCT3EcoRI （類型： Type II restriction enzyme）識別序列：5 GAATTC3HindIII （類型： Type II restriction enzyme）識別序列：5 AAGCTT3KpnI（類型： Type II restriction enzyme）識別序列：5 GGTACC3NcoI（類型： Type II restriction enzyme）識別序列：5 CCATGG3NdeI（類型： Type II restriction enzyme）識別序列：5 CATATG3NheI（類型： Type II restriction enzyme）識別序列：5 GCTAGC3NotI（類型： Type II restriction enzyme）識別序列：5GCGGCCGC 3SacI（類型： Type II restriction enzyme）識別序列：5 GAGCTC3SalI（類型： Type II restriction enzyme）識別序列：5 GTCGAC3SphI（類型：