神經(jīng)生長(zhǎng)因子論文神經(jīng)生長(zhǎng)因子 微球 面神經(jīng)缺損

1、 神經(jīng)生長(zhǎng)因子論文：殼聚糖導(dǎo)管復(fù)合NGF緩釋微球修復(fù)兔面神經(jīng)缺損的實(shí)驗(yàn)研究【中文摘要】研制神經(jīng)生長(zhǎng)因子(nerve growth factor, NGF)緩釋微球,通過微球復(fù)合殼聚糖導(dǎo)管修復(fù)兔面神經(jīng)缺損,從而觀察持續(xù)釋放的NGF在相對(duì)穩(wěn)態(tài)的殼聚糖導(dǎo)管中對(duì)面神經(jīng)生長(zhǎng)的促進(jìn)作用。方法：(1)采用復(fù)乳-溶劑揮發(fā)法(W/O/W)及聚合物合金法與乳化-溶劑揮發(fā)法(S/O/W)結(jié)合制備NGF緩釋微球,對(duì)所制微球一般性質(zhì)及體外釋藥性能進(jìn)行檢測(cè),掃描電鏡觀察微球的形態(tài)、粒徑,NGF含量采用酶聯(lián)免疫吸附測(cè)定(ELISSA)法測(cè)定,釋放液中NGF活性采用PC12細(xì)胞測(cè)定。(2)選擇2.5-3kg的新西蘭大白兔36

2、只,無(wú)菌條件下切斷右側(cè)面神經(jīng)頰支,制成10mm的兔面神經(jīng)頰支缺損模型。完全隨機(jī)分組方法分成四組(每組9只)：A：殼聚糖導(dǎo)管復(fù)合NGF緩釋微球(10mg)組、B：殼聚糖導(dǎo)管復(fù)合單純NGF(5g)組、C：殼聚糖導(dǎo)管復(fù)合生理鹽水組、D：自體神經(jīng)移植組。每組隨機(jī)分為三個(gè)亞組(每個(gè)亞組3只),分別于術(shù)后1個(gè)月,2個(gè)月,3個(gè)月行大體觀察、神經(jīng)直徑測(cè)量、神經(jīng)電生理檢測(cè)、組織化學(xué)和免疫組織化學(xué)和電鏡觀察。結(jié)果：傳統(tǒng)復(fù)乳法NGF緩釋微球包封率(23.74%),聚合物合金法NGF緩釋微球包封率(49.【英文摘要】To prepare the complicated controlled release micro

3、spheres of nerve growth factor (NGF), and respair facial nerve defect in rabbits by using chitosan nerve conduit combined with NGF.To evaluate the role of sustained releasing NGF on the growth of facial nerve.METHODS:(1) The NGF microspheres were obtained by multiple emulsificating solvent evaporati

4、ng(W/O/W) or Polymer-alloys combined with oil-oil emulsion/solvent evaporation (S/O/W)method,and its general properties and releasing of NGF in vit.【關(guān)鍵詞】神經(jīng)生長(zhǎng)因子 微球 面神經(jīng)缺損【英文關(guān)鍵詞】nerve growth factor microsphere the injury of facial nerve【索購(gòu)全文】聯(lián)系Q1：138113721 Q2：139938848 同時(shí)提供論文寫作一對(duì)一輔導(dǎo)和論文發(fā)表服務(wù).保過包發(fā)【目錄】殼聚糖導(dǎo)管復(fù)合NGF緩釋微球修復(fù)兔面神經(jīng)缺損的實(shí)驗(yàn)研究中文摘要5-6英文摘要6-7前言8-11第一部分 NGF微球的制備及特性的研究11-221. 材料和方法11-132. 結(jié)果13-173. 討論17-214. 結(jié)論21-22第二部分 殼聚糖導(dǎo)管復(fù)NGF緩釋微球修復(fù)兔面神經(jīng)缺損的實(shí)驗(yàn)研究22-441. 材料和方法22-252. 結(jié)果25-363. 討論3