標(biāo)注蛋白說明

1、CLProtein NameAccession No.Protein MWProtein PIProteinScoreProteinScoreC. I. %蛋白名稱-3.305130 chloroplast sedoheptulose-1,7-bisphosphatase Morus alba var. multicaulisgi|11817592942824.96.06356100景天庚酮糖-1,7-二磷酸酶桑樹-2.893090 PREDICTED: 30S ribosomal protein 2, chloroplastic-like Fragaria vesca subsp. vesc

2、agi|47014157826303.89.157799.826核糖體蛋白30S野草莓-2.893090 PREDICTED: ribulose-phosphate 3-epimerase,chloroplastic-like Fragaria vesca subsp. vescagi|470126730300558.92165100核酮糖-磷酸-3-差向異構(gòu)酶-2.797190 Probable protein disulfide-isomerase A6 OS=Medicago sativa PE=1 SV=1 40808.85.447999.287二硫鍵異構(gòu)酶-2.797190 Prot

3、ease Do-like 1, chloroplastic OS=Arabidopsis thaliana GN=DEGP1 PE=1 SV=2 46816.56.007698.789Protease Do-like 1-2.781850 glyeraldehyde 3-phosphate dehydrogenase Rosa multifloragi|35325970336539.17.68234100甘油醛-3-磷酸脫氫酶野草莓-2.722660 chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase activase Mor

4、us alba var. multicaulisgi|11985547527345.54.76675100核酮糖-1,5-二磷酸羧化酶/加氧酶-2.344520 PREDICTED: cysteine synthase-like Fragaria vesca subsp. vescagi|470132276345415.498799.983半胱氨酸合成酶白梨-2.344520 PREDICTED: succinyl-CoA ligase ADP-forming subunit alpha-1, mitochondrial-like Fragaria vesca subsp. vescagi|4

5、7012698935348.59.06204100琥珀?；o酶A白梨-2.313030 chloroplast Mn-superoxide dismutase 1B-d Prunuspersicagi|38338610726083.68.579699.998SOD酶桃-2.236500 PREDICTED: 26S protease regulatory subunit 6A homolog A-like Fragaria vesca subsp. vescagi|47013304947833.65.02627100蛋白酶調(diào)控亞基26S野草莓-2.158890 PREDICTED: serin

6、e/arginine-rich splicing factor 33-like Fragaria vesca subsp. vescagi|47011772230062.211.497099.166精氨酸-絲氨酸富集剪接因子33野草莓-1.992800 PREDICTED: 2-dehydro-3-deoxyphosphooctonate aldolase 1-like Fragaria vesca subsp. vescagi|47014166732073.86.61210100醛縮酶型 2脫氫-3-脫氧 野草莓-1.84426methionine sulfoxide reductase M

7、orus alba var. multicaulisgi|37286410021874.76.3362100蛋氨酸硫氧化物還原酶桑樹-1.77162PREDICTED: soluble inorganic pyrophosphatase-like Fragaria vesca subsp. vescagi|47014177824206.45.67149100可溶性無機(jī)焦磷酸酶野草莓-1.77162PREDICTED: gamma carbonic anhydrase 1,mitochondrial-like Fragaria vesca subsp. vescagi|4701112532963

8、3.46.19299.995碳酸酐酶1野草莓-1.67692PREDICTED: 20 kDa chaperonin, chloroplastic-like Fragaria vesca subsp. vescagi|47010243826123.19.03152100伴侶蛋白20 kDa野草莓-1.59956PREDICTED: regulator of ribonuclease-like protein 3-like Fragaria vesca subsp. vescagi|47011744526953.78.599499.997核糖核酸調(diào)節(jié)酶-3野草莓-1.559810 hypothe

9、tical protein PRUPE_ppa006841mg Prunus persicagi|46241995043337.95.68460100S-腺苷甲硫氨酸合成酶桃-1.52408glutathione peroxidase 6, partial Fragaria x ananassagi|47649085724730.79.25153100谷胱甘肽過氧化酶草莓-1.51375actin, partial Malus domesticagi|35532994440369.45.67651100肌動蛋白亞基蘋果1.62532porin Prunus armeniacagi|503127

10、929764.47.057799.822孔蛋白大扁杏1.95422Deoxyuridine 5'-triphosphate nucleotidohydrolase OS=Arabidopsis thaliana GN=DUT PE=1 SV=117603.25.34106100脫氧尿苷5-三磷酸，核苷酸水解酶擬南芥1.967800 Nucleoside diphosphate kinase OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) GN=ndk1 PE=3 SV=11

11、7035.77.8183100核苷二磷酸激酶A粗糙脈孢菌1.967800 Adenylosuccinate synthetase OS=Aquifex aeolicus (strain VF5) GN=purA PE=3 SV=149051.56.027095腺苷酸琥珀酸合成酶桃2.086590 PREDICTED: ATP synthase subunit beta,mitochondrial-like Fragaria vesca subsp. vescagi|47012718359266.96.068699.981ATP合成酶亞基野草莓2.086590 PREDICTED: non-sp

12、ecific lipid-transfer protein-like Fragaria vesca subsp. vescagi|47010965713608.29.576697.704脂轉(zhuǎn)移蛋白非特異性野草莓2.310140 Bifunctional DNA-directed RNA polymerase subunit beta-beta' OS=Wolbachia pipientis wMel GN=rpoBC PE=3 SV=1319977.06.027899.123RNA聚合酶野草莓2.310140 Xylose isomerase OS=Rhizobium loti (st

13、rain MAFF303099) GN=xylA PE=3 SV=1 49383.86.116892.184木糖異構(gòu)酶野草莓2.496840 PREDICTED: DEAD-box ATP-dependent RNA helicase 37-like Fragaria vesca subsp. vescagi|47010486170313.86.435678.573DEAD-box RNA解旋酶水葛2.496840 maturase K Pouzolzia mixtagi|37210009829662.59.525362.763成熟酶K2.496840 PREDICTED: mitochond

14、rial dicarboxylate/tricarboxylate transporter DTC-like Fragaria vesca subsp. vesca gi|47011774332144.69.455784.831二/三羧酸轉(zhuǎn)運蛋白酶野草莓2.964970 Translation initiation factor IF-2 OS=Leuconostoc citreum (strain KM20) GN=infB PE=3 SV=191089.29.188399.71 翻譯起始因子桃2.964970 Mitochondrial inner membrane protease AT

15、P23 OS=Ustilago maydis (strain 521 / FGSC 9021) GN=ATP23 PE=3 SV=1 25174.27.17094.071線粒體內(nèi)膜蛋白酶ATP23玉米黑粉菌2.964970 Kinesin-like protein KIF20B OS=Mus musculus GN=Kif20b PE=1 SV=3204985.75.617296.666紡錘體驅(qū)動蛋白3.128760 mitochondrial NAD-dependent malic acid dehydrogenase Pyrus pyrifoliagi|31544025635604.98.

16、988099.919蘋果酸脫氫酶沙梨3.221110 PREDICTED: ATPase 10, plasma membrane-type-likeFragaria vesca subsp. vescagi|470135969105284.65.346396.01質(zhì)膜ATP酶野草莓3.286490 PREDICTED: protein SEC13 homolog Fragaria vesca subsp. vesca persicagi|47013304133072.15.27183100 SEC13桃從 2 個桑椹蛋白質(zhì)的2DE 凝膠中，切取差異表達(dá)的45個蛋白斑點，經(jīng)膠內(nèi)酶切、質(zhì)譜分析及數(shù)

17、據(jù)庫檢索，結(jié)果成功鑒定了其中的38個蛋白斑點。在對照與感病桑椹中，38個差異表達(dá)蛋白斑點上調(diào)表達(dá)17個，下調(diào)表達(dá)21個。下調(diào)蛋白為，光合作用：景天庚酮糖-1,7-二磷酸酶，Protease Do-like 1，核酮糖-1,5-二磷酸羧化酶/加氧酶，碳酸酐酶1，表達(dá)翻譯：核糖體蛋白30S，二硫鍵異構(gòu)酶，蛋白酶調(diào)控亞基26S，伴侶蛋白20 kDa，代謝過程：核酮糖-磷酸-3-差向異構(gòu)酶，甘油醛-3-磷酸脫氫酶，半胱氨酸合成酶，琥珀?；o酶A，可溶性無機(jī)焦磷酸酶，抗氧化：SOD酶，蛋氨酸硫氧化物還原酶，谷胱甘肽過氧化酶，轉(zhuǎn)錄調(diào)控：精氨酸-絲氨酸富集剪接因子33，核糖核酸調(diào)節(jié)酶-3，水解酶：醛縮酶，逆

18、境相關(guān)：S-腺苷甲硫氨酸合成酶（參與乙烯形成），結(jié)構(gòu)蛋白：肌動蛋白亞基，能量提供減少。光合作用減弱，表達(dá)翻譯減弱，抗氧化能力降低，結(jié)構(gòu)蛋白減少，果實成熟相關(guān)基因表達(dá)降低。上調(diào)表達(dá)蛋白主要為，孔蛋白：結(jié)構(gòu)蛋白1（細(xì)胞膜通透性），SEC13，質(zhì)膜ATP酶，核苷酸水解酶，脫氧尿苷5-三磷酸，糖代謝：蘋果酸脫氫酶，木糖異構(gòu)酶，細(xì)胞分裂：紡錘體驅(qū)動蛋白。代謝過程：線粒體內(nèi)膜蛋白酶ATP23，二/三羧酸轉(zhuǎn)運蛋白酶，ATP合成酶亞基，核苷二磷酸激酶A，翻譯：翻譯起始因子，轉(zhuǎn)錄調(diào)控：成熟酶K ，DEAD-box RNA解旋酶，RNA聚合酶，抗性：脂轉(zhuǎn)移蛋白非特異性，生物合成：腺苷酸琥珀酸合成酶細(xì)胞膜通透性增加

19、，糖被降解利用，核苷酸水解，轉(zhuǎn)錄調(diào)控增加，細(xì)胞分裂加速，部分代謝過程增強(qiáng)， 說明在病原菌侵染過程中，桑葚光合作用，細(xì)胞骨架，部分的代謝過程，及抗氧化能力降低。而細(xì)胞的通透性大大提高，轉(zhuǎn)錄活性增強(qiáng)，細(xì)胞分裂加快，部分代謝途徑加快（糖代謝），抗逆信號增強(qiáng)。 同時還有部分蛋白沒有確定其性能。Protein NameAccession No.Protein MWProtein PIProteinScoreProteinScoreC. I. %蛋白名稱PREDICTED: enolase-like Fragaria vesca subsp. vesca vescagi|47013438848251.65

20、.76365100烯醇化酶（野草莓）ATP synthase CF1 beta subunit Morus indicagi|11480427253815.15.46985100ATP合成酶亞基NAD-dependent malate dehydrogenase Prunus armeniacagi|30770711034811.85.92292100NAD依賴蘋果酸脫氫酶（大扁杏）cytosolic malate dehydrogenase Malus domesticagi|7821649335970.56.01171100胞內(nèi)蘋果酸脫氫酶（蘋果）PREDICTED: pentatrico

21、peptide repeat-containing protein At4g20740-like Fragaria vesca subsp. vescagi|470139321125589.38.756294.618PPR蛋白（野草莓）PREDICTED: oxygen-evolving enhancer protein 1, chloroplastic-like Fragaria vesca subsp. vescagi|470129332353546.1413100放氧增強(qiáng)子蛋白(野草莓)OEE1ribulose bisphosphate carboxylase Malus sp.gi|1

22、958120718.49.048199.932核酮糖-1,5-二磷酸羧化酶（蘋果）chloroplast photosynthetic water oxidation complex 33kDa subunit precursor Morus nigragi|15214364028477.55.48788100葉綠體光合作用水氧化33KDa亞基前體（黑桑莖皮）光合作用水分解反應(yīng)蛋白前體PREDICTED: thioredoxin reductase 2-like Fragaria vesca subsp. vescagi|47010252235769.15.84192100硫氧還蛋白還原酶（野

23、草莓）PREDICTED: coproporphyrinogen-III oxidase, chloroplastic-like Fragaria vesca subsp. vescagi|47013753743834.66.15287100糞卟啉原氧化酶 III (野草莓)PREDICTED: 60S acidic ribosomal protein P2B-like Fragaria vesca subsp. vescagi|470134336114484.5621510060s酸性核糖體蛋白 P2B(野草莓)PREDICTED: 1-acyl-sn-glycerol-3-phosphat

24、e acyltransferase 2-like Fragaria vesca subsp. vescagi|47014014844110.18.026698脂酰甘油3磷酸?；D(zhuǎn)移酶（野草莓）LPAATenoyl-ACP reductase Malus domesticagi|8256944641610.38.728199.931烯酰酸性磷酸酶還原酶（蘋果）cytochrome cgi|22947112149.69.886193.961細(xì)胞色素 cPREDICTED: cytochrome c oxidase subunit 6b-1-like Fragaria vesca subsp. ve

25、scagi|47012742820119.44.33137100細(xì)胞色素c氧化酶亞基(野草莓)PREDICTED: quinone oxidoreductase-like protein At1g23740, chloroplastic-like Fragaria vesca subsp.vescagi|47012323642124.48.73181100醌氧化還原酶蛋白(野草莓)PREDICTED: 2-Cys peroxiredoxin BAS1-like,chloroplastic-like Fragaria vesca subsp. vescagi|47013540228882.96.

26、9199100過氧化物氧化還原酶BAS1(野草莓)putative CC-NBS-LRR resistance protein Malus domesticagi|86438846108432.88.136192.905 CC-NBS-LRR（蘋果）Lea protein precursor Glycine maxgi|35172792349483.77.089399.95lea蛋白前體（大豆）PREDICTED: cysteine synthase-like Fragaria vesca subsp. vescagi|47012627534381.16.06291100半胱氨酸合成酶（野草莓

27、）eugenol synthase 2 Fragaria x ananassagi|54006609934507.16.629699.998丁香酚合成酶2（草莓）chitinase-like protein LA-a, partial Morus albagi|353677822450946.28117100幾丁酶（桑）PREDICTED: thaumatin-like protein-like Fragaria vesca subsp. vescagi|47013792325581.88.327599.724類甜蛋白基因(野草莓)PREDICTED: isoflavone reductase

28、-like protein-like Fragaria vesca subsp. vescagi|47010662862652.16.679399.996類異黃酮還原酶基因蛋白（野草莓）PREDICTED: lipoxygenase homology domain-containing protein 1-like Fragaria vesca subsp. vescagi|47010358519632.54.527699.781脂氧合酶類1。LOXHD1 PREDICTED: NADP-dependent alkenal double bond reductase P2-like Fraga

29、ria vesca subsp. vescagi|47010142941644.75.38299.945 NADP-dependent烯基雙鍵還原酶P2（野草莓）PREDICTED: S-adenosylmethionine synthase 3-like isoform 3 Fragaria vesca subsp. vescagi|47012210343396.95.5497100S-腺苷甲硫氨酸合成酶3亞型3（野草莓）PREDICTED: stromal 70 kDa heat shock-related protein, chloroplastic-like Fragaria vesc

30、a subsp. vescagi|47012944374291.65.13631100熱應(yīng)激蛋白，葉綠素間質(zhì)70（野草莓）PREDICTED: poly(rC)-binding protein 1-like Fragaria vesca subsp. vescagi|470132392576264.8169100poly(rC）結(jié)合蛋白（野草莓）PREDICTED: probable mediator of RNA polymerase II transcription subunit 37e-like Fragaria vesca subsp.vescagi|47013373571279.3

31、5.11708100RNA聚合酶II介質(zhì)轉(zhuǎn)錄亞基（野草莓）retrotransposon protein, putative, Ty3-gypsy subclassgi|77556709150106.68.747496.213反轉(zhuǎn)錄轉(zhuǎn)座子蛋白PREDICTED: LOW QUALITY PROTEIN: probable histone-lysine N-methyltransferase ATXR3-like Fragaria vesca subsp. vescagi|470102953274074.56.636193.378組蛋白賴氨酸甲基轉(zhuǎn)移酶（野草莓）PREDICTED: polyadenylate-binding protein RBP45-like isoform 1 Fragaria vesca subsp. vescagi|47011640246768.35.68123100多聚腺苷酸結(jié)合蛋白 RBP45putative glycine-rich RNA-binding protein Prunus aviumgi|3485112417373.97.82101100富含甘氨酸RNA結(jié)合蛋白（櫻桃）PREDICTED: nascent polypeptide-associated complex subunit alpha-like protein-li