第二章發(fā)酵過程的工藝控制-1

1、第二部分 發(fā)酵工藝控制第二章第二章 發(fā)酵過程的工藝控制發(fā)酵過程的工藝控制第三章第三章 基因工程菌的發(fā)酵基因工程菌的發(fā)酵第四章第四章 動植物細(xì)胞培養(yǎng)動植物細(xì)胞培養(yǎng)0300060009000016487296120Time(h)Titer of CTC(u/ml)0.30.81.31.82.32.83.33.84.3Conc. of NH2-N(g/L)CTC 2g/LCTC 4g/LCTC 6g/LCTC 8g/LCTC 10g/LNH2-N 2g/LNH2-N 4g/LNH2-N 6g/LNH2-N 8g/LNH2-N 10g/LEffect of （NH4）2SO4 on CTC Biosy

2、nthesis(搖瓶搖瓶)0200040006000800018426690120t（ h）CTC（u/ml）353739414345X（10g/L）CTC 0.043g/LCTC 0.087g/LCTC 0.13g/LCTC 0.195g/LX 0.043g/LX 0.087g/LX 0.13g/LX 0.195g/LEffect of KH2PO4 on CTC Biosynthesis0300060009000018407090120Time(h)Titer of CTC(u/ml)00.30.60.91.21.51.8Conc. of NH2-N(g/L)CTC 10g/LCTC 20

3、g/LCTC 29g/LCTC 40g/LCTC 50g/LNH2-N 10g/LNH2-N 20g/LNH2-N 29g/LNH2-N 40g/LNH2-N 50g/LEffect of soybean flour on CTC Biosynthesis030006000900024487296120Time(h)Titer of CTC(u/ml)00.20.40.60.811.21.41.6Conc. of NH2-N(g/l)CTC 1g/LCTC 3g/LCTC 5g/LCTC ControlNH2-N 1g/LNH2-N 3g/LNH2-N 5g/LNH2-N ControlEff

4、ect of yeast extract on CTC Biosynthesis03000600090001200015000180002100016406488112t（h）CTC（u/ml）20304050607080X（*10,g/l） OUR（mmol/h/l）CTC 0.05CTC 0.15X 0.05X 0.15OUR 0.05OUR 0.15The effect of KH2PO4 on CTC fermentation（發(fā)酵罐）（發(fā)酵罐）010203040506082542567390104 121t(h)CER(mmol/l/h)04000800012000160002000

5、0Conc.of CTC(g/ml)CER 1#CER 2#CER 3#CTC 1#CTC 2#CTC 3#The effect of different kinds of yeast flour on CTC fermentation010203040506018426690114t(h)Conc.of biomass(g/l，*10)0500010000150002000025000 Conc.of CTC(g/ml)X 0.0028X 0.0042X 0.0056CTC 0.0028CTC 0.0042CTC 0.0056Effect of different conc. of yeas

6、t extract on CTC fermentation0102030405062441557289103 120t(h)CER(mmol/l/h)040008000120001600020000Conc.of CTC(g/ml)CER 18.5CER 20.6CER 23.3CTC 18.5CTC 20.6CTC 23.3The effect of inoculum age on CTC fermentation（罐）（罐）2030405060163047647895112t/hConc.of X （g/l，*10）10004000700010000130001600019000Conc.of CTC（g/ml）X(%) 10.40%X(%) 14.00%X(%) 17.30%CTC 10.40%CTC 14.00%CTC 17.30%The effect of inoculum amount on cell growth and CTC biosynthesis0