小單孢菌屬及擬諾卡氏菌屬.ppt

稀有放線菌的分離與培養(yǎng) 報(bào)告人 張曉敏 報(bào)告內(nèi)容 分離培養(yǎng)基的選擇小單孢菌形態(tài)與生理生化特征擬諾卡氏菌形態(tài)與生理生化特征小單孢菌 擬諾卡氏菌與鏈霉菌區(qū)別 分離培養(yǎng)基的選擇 淀粉 酪蛋白硝酸鹽瓊脂培養(yǎng)基溶膠幾丁質(zhì)瓊脂培養(yǎng)基甘露醇 利福平瓊脂培養(yǎng)基 淀粉 酪蛋白硝酸鹽瓊脂培養(yǎng)基 starch caseinnitrateagar starch10g sodiumcaseinate0 3g KNO32g agar15g PH7 0 7 4 37 gnovobiocin 223 gmycostatin30 培養(yǎng)21天Micromonosporachaiyaphumensissp novMicromonosporaeburneasp nov 基礎(chǔ)培養(yǎng)基 K2HPO40 7g KH2PO40 3g MgSO4 7H2O0 5g FeSO4 7H2O0 01g ZnSO40 001g 瓊脂10克 蒸餾水1 0L PH7 0121 蒸汽滅菌20min 每100mL加20mL膠體幾丁質(zhì)懸浮液和5mg放線菌酮 溶膠幾丁質(zhì)瓊脂培養(yǎng)基 甘露醇 利福平瓊脂培養(yǎng)基 mannitol rifampicinagar 甘露醇10g 酵母提取物7g 酪蛋白水解物2g 蛋白胨1g NaCl1g CaCO30 2g 制霉菌素100mg 利福平5mg27 培養(yǎng)6周Nocardiaacidivoran 返回 Micromonospora gyrB基因序列分析和DNA DNA雜交15species Micromonosporaaurantiaca Micromonosporacarbonacea Micromonosporachalcea M chersina Micromonosporacoerulea Micromonosporaechinospora Micromonosporagallica Micromonosporahalophytica Micromonosporainositola M matsumotoense Micromonosporanigra M olivasterospora Micromonosporapallida MicromonosporapurpureochromogenesandM rosaria 小單孢菌形態(tài)與生理生化特征 Aerobic Gram positive mesophilic non motileactinomycetewell developedandbranchedsubstratehyphae Aerialmyceliumwasabsent Sporechainswereabsent Mycolicacidswereabsent Acyltypeofcellwallmuramicacidisglycolyl Fatty acidtype 3b Thepredominantmenaquinone MK 9 H4 MK 9 H4H6H8 Characteristicwhole cellsugarsarexyloseandarabinose Phospholipids P II型 Cell wallpeptidoglycan CW II glutamicacid alanineandmeso diaminopimelicacid DNAG C mol 71 73 well developedandbranchedsubstratehyphae Aerialmyceliumwasabsent Atmaturity sporesweresphericaltooval appearedtoberoughandwerenon motile Fig 1 colourofthevegetativemyceliumonISP2mediumisorange turningtogreyishblackorblackuponsporulation growthatpH4 8and25 30 nogrowthabove45 Brown diffusiblepigment positivefortheutilizationofL arabinose D fructose D galactose D glucose lactose melibiose salicinandD xylose negativefornitratereductionandtheutilizationofD mannitolandrhamnose Micromonosporachaiyaphumensis Single non motile sphericalspores 0 6 0 9mm wereformedonsubstratehyphae Aerialmyceliumisabsent Thesporesurfaceappearswarty Sporesarenon motile Thecolourofthespulatingcolonieswasyellowishwhitetopaleorange Ayellow solublepigment UtilizesL arabinose D fructose D galactose D glucose lactose D ribose salicinandD xylose weakutilizationofcellobiose notutilizeglycerol D mannitol melibiose raffinoseorL rhamnose Micromonosporapattaloongensis well developedandbranchedsubstratehyphae Single non motilesporesareformedonsubstratehyphae 0 45mmindiameter Aerialmyceliumisabsent Thespore surfaceappearsroughandnodular Coloniesareyellowishwhiteandturngreyishblackaftersporulation Paleyellowsolublepigment positivefornitratereduction UtilizesD glucose L rhamnose D melibiose D raf nose glycerol salicin lactose cellobioseandD xylose weaklyutilizesD mannitolandD galactosebutnotL arabinose D fructoseorD ribose Micromonosporaeburnea 生理生化區(qū)別 返回 擬諾卡氏菌屬 原擬諾卡氏菌屬包括7個(gè)種2個(gè)亞種 Grund等根據(jù)化學(xué)分類和數(shù)值分類研究