Lin28-let-7a-antagonist-1-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemELin28-let-7aantagonist1Cat.No.:HY-100692CASNo.:2024548-03-4分?式:C??H??N?O?分?量:583.59作?靶點:MicroRNA作?通路:Epigenetics儲存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實驗DMSO:100mg/mL(171.35mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM1.7135mL8.5677mL17.1353mL5mM0.3427mL1.7135mL3.4271mL10mM0.1714mL0.8568mL1.7135mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時，請在6個?內(nèi)使?，-20°C儲存時，請在1個?內(nèi)使?。BIOLOGICALACTIVITY?物活性Lin28-let-7aantagonist1對Lin28-let-7a的結(jié)合有明顯的拮抗作?，其對Lin28A-let-7a-1結(jié)合的IC50值為4.03μM。IC50&TargetIC50：4.03μM(Lin28A-let-7a-1)[1].1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE體外研究Lin28-let-7aantagonist1(compound1)showsaclearantagonisticeffectagainsttheLin28A-let-7a-1interactionwithanIC50of4.03μM.Interestingly,Lin28-let-7aantagonist1alsoinhibitstheLin28B-let-7interaction,withslightlyreducedpotency.DicerprocessingassayrevealsthatLin28-let-7aantagonist1clearlyblockstheformationoftheLin28A-pre-let-7gcomplexandinducesmiRNAprocessing,resultinginthegenerationofmaturelet-7gmiRNA.WhentheexpressionofbothLin28AandLin28Bareknocked-downusingsiRNAinJARcells,theLin28-let-7aantagonist1-inducedincreaseincellularlet-7levelsisclearlyattenuated.Lin28A-let-7a-IN-1increasesmaturelet-7levelsinPA-1cellsthatexpresshighlevelofLin28A.Ontheotherhand,let-7levelsarenotaffectedby1inMCF7cellsthatrarelyexpressLin28proteins.Takentogether,Lin28-let-7aantagonist1specificallyinducestheincreaseinthecellularlet-7levelsbytargetingLin28[1].戶使?本產(chǎn)品發(fā)表的科研?獻?MolCell.2021Oct12;S1097-2765(21)00796-6.?Oncogenesis.2022Jul2;11(1):37.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].LimD,etal.DiscoveryofaSmall-MoleculeInhibitorofProtein-MicroRNAInteractionUsingBindingAssaywithaSite-SpecificallyLabeledLin28.JAmChemSoc.2016Oct7.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonl