Hygromycin-B-Hygrovetine-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEHygromycinBCat.No.:HY-B0490CASNo.:31282-04-9Synonyms:Hygrovetine分?式:C??H??N?O??分?量:527.52作?靶點(diǎn):Bacterial;Fungal;Antibiotic;Parasite作?通路:Anti-infection儲(chǔ)存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:100mg/mL(189.57mM;ultrasonicandwarmingandheatto60°C)H2O:50mg/mL(94.78mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM1.8957mL9.4783mL18.9566mL5mM0.3791mL1.8957mL3.7913mL10mM0.1896mL0.9478mL1.8957mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE1.請(qǐng)依序添加每種溶劑：1%DMSO>>99%salineSolubility:≥0.5mg/mL(0.95mM);Clearsolution2.請(qǐng)依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥5mg/mL(9.48mM);Clearsolution3.請(qǐng)依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥5mg/mL(9.48mM);Clearsolution4.請(qǐng)依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥5mg/mL(9.48mM);Clearsolution5.請(qǐng)依序添加每種溶劑：5%DMSO>>40%PEG300>>5%Tween-80>>50%salineSolubility:≥2.5mg/mL(4.74mM);Clearsolution6.請(qǐng)依序添加每種溶劑：5%DMSO>>95%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(4.74mM);Clearsolution7.請(qǐng)依序添加每種溶劑：PBSSolubility:100mg/mL(189.57mM);Clearsolution;NeedultrasonicBIOLOGICALACTIVITY?物活性HygromycinB抑制原核和真核細(xì)胞的氨糖苷類抗?素。IC50&TargetAminoglycoside體外研究HygromycinB,anaminocyclitolantibioticthatstronglyinhibitsboth70Sand80Sribosomes,issynthesizedbyStreptomyceshygroscopicus[1].HygromycinBat0.38mMconcentrationcompletelyhaltsyeastcellgrowthinrichmedia,presumablybypreventingproteinsynthesisbycytoplasmicribosomes.Polypeptidesynthesisincell-freeextractsfromrabbitreticulocytes,wheatgermandyeastisstronglyblockedbylowconcentrationsofhygromycinB.TheantibioticinhibitspeptidechainelongationbyyeastpolysomesbypreventingelongationfactorEF-2-dependenttranslocation.TheinhibitionoftranslocationbyhygromycinBmightresultfromthestabilizationofpeptidyl-tRNAboundtotheribosomalacceptorsite[2].體內(nèi)研究HygromycinBinhibitsproteinsynthesisbyblockingribosomaltranslocationwithoutcausingsignificantmisreadinginvivo[3].ConstitutiveexpressionofthebacterialhygRgeneintransgenicmiceinvivoconfersresistancetohygromycinB[4].PROTOCOLAnimalHygromycinBisdissolvedinsterilewater.ThemiceC57BL/6J-TgN(pPWL512hyg)1EmscarryinghygRareAdministration[4]treatedwithasingledoseofhygromycinBi.p.atdosesthatstartsat2.7mg/kgandincreasesby50%foreachconsecutivedose.Controlwild-typeC57BL/6Jmicearetreatedwiththesamevolumeofsterilesaline.Totalvolumeinjectedis0.5mL.Thehealthstatusandbodyweightsofanimalsaremonitoreddailyfor10consecutivedays[4].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?AntimicrobAgentsChemother.2022Nov10;e0097722.?AmJTranslRes.2021Apr15;13(4):2331-2349.?BiochemBiophysResCommun.6August2022.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].PardoJM,etal.BiochemicalbasisofresistancetohygromycinBinStreptomyceshygroscopicus--theproducingorganism.JGenMicrobiol.1985Jun;131(6):1289-98.[2].GonzálezA,etal.StudiesonthemodeofactionofhygromycinB,aninhibitoroftranslocationineukaryotes.BiochimBiophysActa.1978Dec21;521(2):459-69.[3].PfisterP,etal.Roleof16SrRNAHelix44inRibosomalResistancetoHygromycinB.AntimicrobAgentsChemother.2003May;47(5):1496-502.McePdfHei