Protein-deglycase-DJ-1-against-1-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEProteindeglycaseDJ-1against-1Cat.No.:HY-137262CASNo.:724737-74-0分?式:C??H??N?O?分?量:417.46作?靶點(diǎn):Others作?通路:Others儲(chǔ)存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:25mg/mL(59.89mM;ultrasonicandadjustpHto7withNaOH)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM2.3954mL11.9772mL23.9544mL5mM0.4791mL2.3954mL4.7909mL10mM0.2395mL1.1977mL2.3954mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.08mg/mL(4.98mM);Clearsolution1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE2.請(qǐng)依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(4.98mM);ClearsolutionBIOLOGICALACTIVITY?物活性ProteindeglycaseDJ-1against-1DJ-1結(jié)合化合物，依賴地靶向DJ1。ProteindeglycaseDJ-1against-1可以穿透?腦屏障(BBB)。ProteindeglycaseDJ-1against-1神經(jīng)保護(hù)劑，具有?于帕?森病研究的潛?。體內(nèi)研究ProteindeglycaseDJ-1against-1(compound-23;1mg/kg;IP;pretreatmentonehour;daily;for4days)significantlyrestoresMPTPinducedlocomotion,dopaminergicneuronalcelldeathanddopaminecontentinDJ-1(+/+)micebutnotinDJ-1(-/-)mice[1].AnimalModel:Wild-typemaleC57BL/6miceat8,11-12and9-12weeks;DJ-1-knockoutmalemiceat11-13weeks[1]Dosage:1mg/kgAdministration:IP;pretreatmentonehour;daily;for4daysResult:SignificantlyrestoredMPTP(30mg/kg;IP;Onehourafterinjection)inducedlocomotion,dopaminergicneuronalcelldeathanddopaminecontentinDJ-1(+/+)micebutnotinDJ-1(-/-)mice.REFERENCES[1].KazukoTakahashi-Niki,etal.DJ-1-dependentprotectiveactivityofDJ-1-bindingcompoundno.23againstneuronalcelldeathinMPTP-treatedmousemodelofParkinson'sdisease.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.T