不飽和脂肪酸及神經(jīng)生長(zhǎng)因子對(duì)神經(jīng)細(xì)胞之影響課件

健腦食品中有效成分對(duì)神經(jīng)細(xì)胞之影響第一組

B9632078何純誼(乙)

B9632083王泓政(甲)指導(dǎo)老師：陳淑德老師2010/5/101健腦食品中有效成分對(duì)神經(jīng)細(xì)胞之影響第一組

B9632078大綱前言銀杏提取物對(duì)腦部功能及神經(jīng)保護(hù)之影響DHA對(duì)神經(jīng)細(xì)胞之影響猴頭菇對(duì)神經(jīng)細(xì)胞之影響L-肉鹼對(duì)神經(jīng)細(xì)胞之影響結(jié)論2010/5/102大綱前言2010/5/102(廖等,2008)健腦食品許多食品中的成分均被強(qiáng)調(diào)有助於腦部發(fā)展、維持敏銳的腦力、增強(qiáng)學(xué)習(xí)效果與認(rèn)知能力及避免罹患「老人癡呆癥」常被提到的有ω-3、卵磷脂、左旋肉鹼、銀杏、猴頭菇…等

2010/5/103健腦食品許多食品中的成分均被強(qiáng)調(diào)有助於腦部發(fā)展、維持敏(童等,2008)神經(jīng)細(xì)胞神經(jīng)細(xì)胞基本由細(xì)胞本體、樹(shù)突及軸突構(gòu)成，周圍各分佈不同的支持性細(xì)胞神經(jīng)細(xì)胞可由活體動(dòng)物中直接取出培養(yǎng)或購(gòu)買細(xì)胞株在37℃、5~10%CO2下進(jìn)行培養(yǎng)2010/5/104(童等,2008)神經(jīng)細(xì)胞神經(jīng)細(xì)胞基本由細(xì)胞本體、樹(shù)(Robsonetal.,2010)由其生長(zhǎng)分化型態(tài)可分為四種背根神經(jīng)節(jié)(DRG)神經(jīng)元生長(zhǎng)情形2010/5/105Fig.1DRGneuronesfromadultanimalsandtheiroutgrowthpattern.Scalebar:50μm.(Robsonetal.,2010)由其生長(zhǎng)分化型態(tài)可Growthcone(生長(zhǎng)錐)當(dāng)神經(jīng)細(xì)胞經(jīng)過(guò)了一連串的分裂與移動(dòng)之後，細(xì)胞就會(huì)開(kāi)始進(jìn)行最後一個(gè)步驟--細(xì)胞分化。在神經(jīng)分化的早期，軸突頂端會(huì)有生長(zhǎng)錐(growthcone)延伸出來(lái)，其功能在於引導(dǎo)兩個(gè)不同區(qū)域的神經(jīng)互相連接。2010/5/106.tw/lesson3/nerv2.htmGrowthcone(生長(zhǎng)錐)當(dāng)神經(jīng)細(xì)胞經(jīng)過(guò)了一連串的分神經(jīng)生長(zhǎng)因子(NGF)可誘導(dǎo)生長(zhǎng)錐趨化性生長(zhǎng)的一個(gè)重要因子就是神經(jīng)生長(zhǎng)因子(NGF)，它是蛋白質(zhì)?？山?jīng)由細(xì)胞膜上特殊的受體可進(jìn)一步誘導(dǎo)神經(jīng)節(jié)迅速生長(zhǎng)，並促使神經(jīng)纖維大量分支?？稍黾游闯墒旒?xì)胞的存活率?？砂缪菡T導(dǎo)神經(jīng)纖維向其作用器官生長(zhǎng)的功能

2010/5/107/jw!ZKwmgZyFGRsJ4s4XMfTTO3Muh3xK4s.DwUU-/article?mid=157神經(jīng)生長(zhǎng)因子(NGF)可誘導(dǎo)生長(zhǎng)錐趨化性生長(zhǎng)的一(李等,2010)銀杏提取物(EGB)對(duì)腦部神經(jīng)保護(hù)之影響銀杏葉提取物(extractofGinkgobiloba,EGB)，內(nèi)含22～27%的銀杏黃酮與5～7%的銀杏苦內(nèi)酯。銀杏提取物是一有效的抗氧化劑和自由基清除劑，對(duì)自由基引起的細(xì)胞凋亡具有明顯的抑制作用。2010/5/108.tw/main.php?method=article&id=66(李等,2010)銀杏提取物(EGB)對(duì)腦部神經(jīng)保護(hù)之影(李等,2010)銀杏萃取物對(duì)治療12週糖尿病大鼠腦神經(jīng)密度之影響2010/5/109Fig.2Comparisonoftheneuroaldensitiesofhippocampus.*P<0.05,△△P<0.01vscontrolgroup(李等,2010)銀杏萃取物對(duì)治療12週糖尿病大鼠腦神經(jīng)(李等,2010)銀杏萃取物對(duì)大鼠記憶及認(rèn)知學(xué)習(xí)能力測(cè)驗(yàn)之影響2010/5/1010Fig.3TheeffectofEGBontheplatformsearchingscoreofMorriswatermazetest.**P<0.01vscontrolgroup(李等,2010)銀杏萃取物對(duì)大鼠記憶及認(rèn)知學(xué)習(xí)能力測(cè)驗(yàn)DHA對(duì)神經(jīng)細(xì)胞之影響二十二碳六烯酸(Docosahexaenoicacid,DHA)是在哺乳動(dòng)物大腦及神經(jīng)細(xì)胞膜上發(fā)現(xiàn)最豐富的不飽和脂肪酸。對(duì)正常腦部及神經(jīng)系統(tǒng)之成長(zhǎng)與發(fā)展是重要的。沮喪、癡呆與阿茲海默癥也與ω-3脂肪酸有關(guān)。2010/5/1011(Cao

etal.,2005)(Limetal.,2005)DHA對(duì)神經(jīng)細(xì)胞之影響二十二碳六烯酸(Docosahexa(Robsonetal.,2010)不飽和脂肪酸添加於神經(jīng)細(xì)胞培養(yǎng)2010/5/1012↑162%↓43%+49%↑117%↑15%↓41%↓73%+42%+5%↑42%↑174%↑80%↑166%Table1EffectofNGF,AA,EPAandDHAonneuriteoutgrowthinfemaleDRG(Robsonetal.,2010)不飽和脂肪酸添加於(Robsonetal.,2010)不飽和脂肪酸添加於神經(jīng)細(xì)胞培養(yǎng)2010/5/1013↑34%↑73%↑141%↑136%Fig.4EffectofAA,EPA,DHA,NGFontotalandmaximumneuritegrowthinDRGfromoldfemalerats.(Robsonetal.,2010)不飽和脂肪酸添加於DHA&NGF對(duì)兔子角膜受損之治療2010/5/1014Fig.5Imagesofrabbitcornealsectionstaken8weeksafterPRKsurgeryandtreatedwithNGF,DHAorNGFplusDHA.(Heetal.,2010)DHA&NGF對(duì)兔子角膜受損之治療2010/5/1014(Heetal.,2010)2010/5/1015Fig.6Ki-67stainingofthebasalcornealepithelialcells(arrows)ofrabbits8weeksaftersurgeryandtreatedwithNGF,DHAorNGFplusDHADHA&NGF對(duì)兔子角膜受損之治療(Heetal.,2010)2010/5/1015Fi猴頭菇對(duì)神經(jīng)細(xì)胞之影響猴頭蘑，又稱猴頭菇、刺蝟菌、蝟菌，擔(dān)子菌綱，齒菌科其中的猴頭素含有可促進(jìn)神經(jīng)生長(zhǎng)因子(NGF)合成之細(xì)胞機(jī)能調(diào)節(jié)物質(zhì)2010/5/1016.tw/label.aspx?article=957猴頭菇對(duì)神經(jīng)細(xì)胞之影響猴頭蘑，又稱猴頭菇、刺蝟菌、蝟菌，擔(dān)子(劉等,2008)猴頭菌萃取物對(duì)衰老小鼠記憶能力的影響2010/5/1017Table2EffectofHEonmemorycapacitiesofagedmice(劉等,2008)猴頭菌萃取物對(duì)衰老小鼠記憶能力的影響2(Park

etal.,2002)添加猴頭菌萃取物培養(yǎng)神經(jīng)細(xì)胞

(a)Fig.7EffectofHEonnervecelloutgrowth.

(b)2010/5/1018(Parketal.,2002)添加猴頭菌萃取物培養(yǎng)神L-肉鹼對(duì)腦神經(jīng)細(xì)胞之影響左旋肉鹼是由人體內(nèi)的兩種胺基酸：離胺酸與蛋胺酸之前驅(qū)物所合成為運(yùn)輸長(zhǎng)鏈脂肪酸進(jìn)入細(xì)胞中的粒腺體內(nèi)及代謝脂肪酸產(chǎn)生能量2010/5/1019(廖等,2008)

L-肉鹼結(jié)構(gòu)圖L-肉鹼對(duì)腦神經(jīng)細(xì)胞之影響左旋肉鹼是由人體內(nèi)的兩種胺基酸：離L-肉鹼對(duì)年輕大鼠腦組織RNA和DNA含量2010/5/1020(Julietetal.,2005)Table3EffectofL-carinitineonRNAandDNAstatusofyoungratbrainL-肉鹼對(duì)年輕大鼠腦組織RNA和DNA含量2010/5/10L-肉鹼對(duì)老化大鼠腦組織RNA和DNA含量2010/5/1021Table4EffectofL-carinitineonRNAandDNAstatusofagedratbrain(Julietetal.,2005)L-肉鹼對(duì)老化大鼠腦組織RNA和DNA含量2010/5/10L-肉鹼對(duì)MPP+誘導(dǎo)大鼠前腦神經(jīng)細(xì)胞凋亡培養(yǎng)2010/5/10(Wang

etal.,2007)22Fig.9ImmunofluorescencemicrographsindicatingtheeffectofMPP+andL-carnitineonthePSA-NCAMexpression,aneuron-specificmarker,inratforebraincultures.L-肉鹼對(duì)MPP+誘導(dǎo)大鼠前腦神經(jīng)細(xì)胞凋亡培養(yǎng)2010/5/結(jié)論銀杏萃取物可保護(hù)及改善糖尿病造成之腦部神經(jīng)損傷。DHA可有效增加及修復(fù)神經(jīng)突觸及數(shù)量。猴頭菇可改善衰老小鼠記憶能力。L-肉鹼可提高腦組織DNA及RNA含量。2010/5/1023結(jié)論銀杏萃取物可保護(hù)及改善糖尿病造成之腦部神經(jīng)損傷。.tw/main.php?method=article&id=66Thankforyour

attention2010/5/1024.tw/main.ph健腦食品中有效成分對(duì)神經(jīng)細(xì)胞之影響第一組

B9632078何純誼(乙)

B9632083王泓政(甲)指導(dǎo)老師：陳淑德老師2010/5/1025健腦食品中有效成分對(duì)神經(jīng)細(xì)胞之影響第一組

B9632078大綱前言銀杏提取物對(duì)腦部功能及神經(jīng)保護(hù)之影響DHA對(duì)神經(jīng)細(xì)胞之影響猴頭菇對(duì)神經(jīng)細(xì)胞之影響L-肉鹼對(duì)神經(jīng)細(xì)胞之影響結(jié)論2010/5/1026大綱前言2010/5/102(廖等,2008)健腦食品許多食品中的成分均被強(qiáng)調(diào)有助於腦部發(fā)展、維持敏銳的腦力、增強(qiáng)學(xué)習(xí)效果與認(rèn)知能力及避免罹患「老人癡呆癥」常被提到的有ω-3、卵磷脂、左旋肉鹼、銀杏、猴頭菇…等

2010/5/1027健腦食品許多食品中的成分均被強(qiáng)調(diào)有助於腦部發(fā)展、維持敏(童等,2008)神經(jīng)細(xì)胞神經(jīng)細(xì)胞基本由細(xì)胞本體、樹(shù)突及軸突構(gòu)成，周圍各分佈不同的支持性細(xì)胞神經(jīng)細(xì)胞可由活體動(dòng)物中直接取出培養(yǎng)或購(gòu)買細(xì)胞株在37℃、5~10%CO2下進(jìn)行培養(yǎng)2010/5/1028(童等,2008)神經(jīng)細(xì)胞神經(jīng)細(xì)胞基本由細(xì)胞本體、樹(shù)(Robsonetal.,2010)由其生長(zhǎng)分化型態(tài)可分為四種背根神經(jīng)節(jié)(DRG)神經(jīng)元生長(zhǎng)情形2010/5/1029Fig.1DRGneuronesfromadultanimalsandtheiroutgrowthpattern.Scalebar:50μm.(Robsonetal.,2010)由其生長(zhǎng)分化型態(tài)可Growthcone(生長(zhǎng)錐)當(dāng)神經(jīng)細(xì)胞經(jīng)過(guò)了一連串的分裂與移動(dòng)之後，細(xì)胞就會(huì)開(kāi)始進(jìn)行最後一個(gè)步驟--細(xì)胞分化。在神經(jīng)分化的早期，軸突頂端會(huì)有生長(zhǎng)錐(growthcone)延伸出來(lái)，其功能在於引導(dǎo)兩個(gè)不同區(qū)域的神經(jīng)互相連接。2010/5/1030.tw/lesson3/nerv2.htmGrowthcone(生長(zhǎng)錐)當(dāng)神經(jīng)細(xì)胞經(jīng)過(guò)了一連串的分神經(jīng)生長(zhǎng)因子(NGF)可誘導(dǎo)生長(zhǎng)錐趨化性生長(zhǎng)的一個(gè)重要因子就是神經(jīng)生長(zhǎng)因子(NGF)，它是蛋白質(zhì)。可經(jīng)由細(xì)胞膜上特殊的受體可進(jìn)一步誘導(dǎo)神經(jīng)節(jié)迅速生長(zhǎng)，並促使神經(jīng)纖維大量分支?？稍黾游闯墒旒?xì)胞的存活率?？砂缪菡T導(dǎo)神經(jīng)纖維向其作用器官生長(zhǎng)的功能

2010/5/1031/jw!ZKwmgZyFGRsJ4s4XMfTTO3Muh3xK4s.DwUU-/article?mid=157神經(jīng)生長(zhǎng)因子(NGF)可誘導(dǎo)生長(zhǎng)錐趨化性生長(zhǎng)的一(李等,2010)銀杏提取物(EGB)對(duì)腦部神經(jīng)保護(hù)之影響銀杏葉提取物(extractofGinkgobiloba,EGB)，內(nèi)含22～27%的銀杏黃酮與5～7%的銀杏苦內(nèi)酯。銀杏提取物是一有效的抗氧化劑和自由基清除劑，對(duì)自由基引起的細(xì)胞凋亡具有明顯的抑制作用。2010/5/1032.tw/main.php?method=article&id=66(李等,2010)銀杏提取物(EGB)對(duì)腦部神經(jīng)保護(hù)之影(李等,2010)銀杏萃取物對(duì)治療12週糖尿病大鼠腦神經(jīng)密度之影響2010/5/1033Fig.2Comparisonoftheneuroaldensitiesofhippocampus.*P<0.05,△△P<0.01vscontrolgroup(李等,2010)銀杏萃取物對(duì)治療12週糖尿病大鼠腦神經(jīng)(李等,2010)銀杏萃取物對(duì)大鼠記憶及認(rèn)知學(xué)習(xí)能力測(cè)驗(yàn)之影響2010/5/1034Fig.3TheeffectofEGBontheplatformsearchingscoreofMorriswatermazetest.**P<0.01vscontrolgroup(李等,2010)銀杏萃取物對(duì)大鼠記憶及認(rèn)知學(xué)習(xí)能力測(cè)驗(yàn)DHA對(duì)神經(jīng)細(xì)胞之影響二十二碳六烯酸(Docosahexaenoicacid,DHA)是在哺乳動(dòng)物大腦及神經(jīng)細(xì)胞膜上發(fā)現(xiàn)最豐富的不飽和脂肪酸。對(duì)正常腦部及神經(jīng)系統(tǒng)之成長(zhǎng)與發(fā)展是重要的。沮喪、癡呆與阿茲海默癥也與ω-3脂肪酸有關(guān)。2010/5/1035(Cao

etal.,2005)(Limetal.,2005)DHA對(duì)神經(jīng)細(xì)胞之影響二十二碳六烯酸(Docosahexa(Robsonetal.,2010)不飽和脂肪酸添加於神經(jīng)細(xì)胞培養(yǎng)2010/5/1036↑162%↓43%+49%↑117%↑15%↓41%↓73%+42%+5%↑42%↑174%↑80%↑166%Table1EffectofNGF,AA,EPAandDHAonneuriteoutgrowthinfemaleDRG(Robsonetal.,2010)不飽和脂肪酸添加於(Robsonetal.,2010)不飽和脂肪酸添加於神經(jīng)細(xì)胞培養(yǎng)2010/5/1037↑34%↑73%↑141%↑136%Fig.4EffectofAA,EPA,DHA,NGFontotalandmaximumneuritegrowthinDRGfromoldfemalerats.(Robsonetal.,2010)不飽和脂肪酸添加於DHA&NGF對(duì)兔子角膜受損之治療2010/5/1038Fig.5Imagesofrabbitcornealsectionstaken8weeksafterPRKsurgeryandtreatedwithNGF,DHAorNGFplusDHA.(Heetal.,2010)DHA&NGF對(duì)兔子角膜受損之治療2010/5/1014(Heetal.,2010)2010/5/1039Fig.6Ki-67stainingofthebasalcornealepithelialcells(arrows)ofrabbits8weeksaftersurgeryandtreatedwithNGF,DHAorNGFplusDHADHA&NGF對(duì)兔子角膜受損之治療(Heetal.,2010)2010/5/1015Fi猴頭菇對(duì)神經(jīng)細(xì)胞之影響猴頭蘑，又稱猴頭菇、刺蝟菌、蝟菌，擔(dān)子菌綱，齒菌科其中的猴頭素含有可促進(jìn)神經(jīng)生長(zhǎng)因子(NGF)合成之細(xì)胞機(jī)能調(diào)節(jié)物質(zhì)2010/5/1040.tw/label.aspx?article=957猴頭菇對(duì)神經(jīng)細(xì)胞之影響猴頭蘑，又稱猴頭菇、刺蝟菌、蝟菌，擔(dān)子(劉等,2008)猴頭菌萃取物對(duì)衰老小鼠記憶能力的影響2010/5/1041Table2EffectofHEonmemorycapacitiesofagedmice(劉等,2008)猴頭菌萃取物對(duì)衰老小鼠記憶能力的影響2(Park

etal.,2002)添加猴頭菌萃取物培養(yǎng)神經(jīng)細(xì)胞

(a)Fig.7EffectofHEonnervecelloutgrowth.

(b)2010/5/1042(Parketal.,2002)添加猴頭菌萃取物培養(yǎng)神L-肉鹼對(duì)腦神經(jīng)細(xì)胞之影響左旋肉鹼是由人體內(nèi)的兩種胺基酸：離胺酸與蛋胺酸之前驅(qū)物所合成為運(yùn)輸長(zhǎng)鏈脂肪酸進(jìn)入細(xì)胞中的粒腺體內(nèi)及代謝脂肪酸產(chǎn)生能量2010/5/1043(廖等,2008)

L-肉鹼結(jié)構(gòu)圖L-肉鹼對(duì)