Sodium-copper-chlorophyllin-B _HIV 抑制劑 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemESodium copper chlorophyllin BCat. No.: HY-B2226CAS No.: 28302-36-5分式: CHCuNNaO分量: 738.13作靶點(diǎn): HIV; Influenza Virus作通路: Anti-infection儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) H2O : 10 mg/mL (

2、13.55 mM; Need ultrasonic)DMSO : 6.67 mg/mL (9.04 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 1.3548 mL 6.7739 mL 13.5477 mL5 mM 0.2710 mL 1.3548 mL 2.7095 mL10 mM 0.1355 mL 0.6774 mL 1.3548 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請注意儲(chǔ)備液的保存式和期限。體內(nèi)實(shí)驗(yàn)請根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥式選擇適當(dāng)?shù)娜芙獍?，配制前請先配制澄清?/p>

3、儲(chǔ)備液，再依次添加助溶劑(為保證實(shí)驗(yàn)結(jié)果的可靠性，體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使；澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占)：1. 請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 0.67 mg/mL (0.91 mM); Clear solution2. 請依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 0.67 mg/mL (0.91 mM); Clear solution3. 請依序添加

4、每種溶劑： 10% DMSO 90% corn oil1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemESolubility: 0.67 mg/mL (0.91 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Sodium copper chlorophyllin B 對流感病毒和 HIV 發(fā)揮抗病毒活性，其 IC50s 值都是50 到 100 M。IC50 & Target IC50: 50 to 100 M (Influenza virus), 50 to 100 M (HIV) 1體外研究 Sodium

5、copper chlorophyllin B exhibits influenza virus adsorption at 200 M (P50 and the 50% cytotoxicconcentration (CC50) of Sodium copper chlorophyllin lay from 50 to 100 M and 200 to 400 M, respectively.Sodium copper chlorophyllin B also inhibits HIV adsorption at 2.5 mM (P50 and CC50 of Sodium copperchl

6、orophyllin lay from 50 to 100 M and 200 to 400 M, respectively 1.PROTOCOLCell Assay 1 MT-2 cells are pre-incubated to the bamboo leaf extract solution (up to 10%), Sodium copper chlorophyllin(up to 5 mM) or test reagent-free culture medium for 10 min to assess the inhibitory activity at adsorption.T

7、he pre-incubated cells are washed extensively to be able to eliminate antiviral activity of test reagentsremaining in the culture medium, and then they are exposed to HIV-1LAI (1.310-4 TCID50 per cell). Inaddition to these pre-incubated cells, control wells are prepared to evaluate growth of the cel

8、ls in a virus-freecondition. Then, virus-exposed and mock-exposed cells are cultured in the absence of test reagents for threedays. The total viable cells are counted on day 3 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Akiko Ito, et al. In Vitro Inhibition of Cytopathic Effect of Influenza Virus and Human Immunodeficiency Virus by Bamboo Leaf ExtractSolution and Sodium Copper Chlorophyllin. Yonago Acta Med. 2016 Mar; 59(1): 61-65.McePdfHeightCaution: Product has not been fully validated for medical ap