金屬硫蛋白過表達對乳腺癌化療方案選擇的影響

1、金屬硫蛋白過表達對乳腺癌化療方案選擇的影響         11-03-27 16:22:00     編輯：studa20                    作者：董建峰 馬潔華 張輝 張曉麗 薄愛華【摘要】  目的：探討金屬硫蛋白(Metallothionein，MT)在

2、乳腺癌中過表達的意義及其與乳腺癌化療方案選擇的相關(guān)性。方法：利用SP免疫組織化學技術(shù)，檢測88例乳腺癌組織中MT表達率。結(jié)果：MT在乳腺癌中總陽性率為67.05%(59/88)。MT在髓樣癌、小葉癌和導(dǎo)管癌中的陽性率分別為41.67%，85.71%和62.50%。在浸潤型乳腺癌組陽性率明顯高于非浸潤型癌組(P<0.05);在小葉癌組陽性率高于髓樣癌組和導(dǎo)管癌組(P<0.05);淋巴結(jié)轉(zhuǎn)移組陽性率高于非轉(zhuǎn)移組(P<0.05)。結(jié)論：MT表達與乳腺癌的病理類型及有否淋巴結(jié)轉(zhuǎn)移相關(guān)，檢測MT對乳腺癌化療方案的制定有指導(dǎo)意義。 【關(guān)鍵詞】  金屬硫蛋白;乳腺腫瘤;免疫組織化

3、學Since the first report of metallothionein(MT)overexpression in thyroid cancer tissues by Cherian and Nartey in 19871,there has been an extensive interest in the role which is played by MT in tumorigenesis.The abnormal increase of MT is related to the genesis,development and drugresistance of tumor2

4、.To investigate the expression of MT in breast cancer and its clinical significance,we collected 88 cases with breast cancer to examine the expression of MT with streptavidin peroxidase(SP)immunohistochemical method,The aim was to clarify the relation between the expression of MT and the biology cha

5、racteristic of tumor cell,and offer a base of establishing chemistry treatment scheme.1 Materials and methods1.1 Patients and samples88 cases with breast cancer in women were collected from January 2005 to October 2008 at No.1 Affiliated Hospital Hebei North University in Zhangjiakou.The age range w

6、as 30 to 79 years old,the average age was 51.5 years,and the median age was 48.5 years.1.2 Immunohistochemical stainingMouse antimetallothionein antibody was conducted by Santa Cruz,USA.Immunohistochemical staining SP kit and DAB kit were purchased from Beijing Zhongshan Goldenbridge biotechnology C

7、o.Ltd.(China).The samples of cancer were fixed in 100mL/L dehydrated formaldehyde and embedded in paraffin,sections of 5m thickness were sliced,which were dyed by HE staining method,and all samples were confirmed by pathological diagnosis and clinical classitication.Immunohistochemical SP staining m

8、ethod was used in the experiment with conventional staining procedures.The negative control was designed as PBS instead of antibody I,the positive control was known as positive tissue sections which was provided by Beijing Zhongshan Co.Ltd.(China).1.3 Statistical analysis2 test was used for statisti

9、cal analysis,P value less than 0.05 was considered as statistical significance.2 ResultsThe MT immunoreactive productions was showed brown with yellow color and distributed in cytoplasm of the cancer cells.But the cell nucleus and negative control cell were showed no color(Fig13).The relationship be

10、tween the expression of MT and the biology characteristic of carcinomer cell was showed in Tables 1.Table 1 Relationship between MT expression and pathology type,lymph metastases in breast cancerGroupnBreast Cancer MT(+)n%Total positive rate885967.05Marrow cancer12541.67Lobular cancer282485.71Ductal

11、 carcinoma483062.50Infiltrating cancer 685276.47Noninfiltrating cancer20735.00Lymph metastases393076.92Nonlymph metastases492959.18:Positive rate was significantly higher than that of marrow cancer group and ductal carcinoma group(P<0.05);:Positive rate was significantly higher than that of nonin

12、filtrating cancer group(P<0.05);:Positive rate was significantly higher than that of Nonlymph metastases group(P<0.05)Fig 1 Infiltrating ductal carcinoma (SP,×400)Fig 2 Infiltrating lobular cancer (SP,×400)Fig 3 Marrow cancer (SP,×400) DiscussionThe MT is protein of small molecu

13、lar with much sulfydryl.MT gene is located at chromosome 16.MT proteins are small molecular weight proteins containing 61 to 68 amino acid residue,and are characterized by high cysteine content with a paucity of aromatic acids,there is 25%30% cysteine of in MT.In human,there are four subgroup of MT

14、proteins,namely MT1,MT2,MT3 and MT4,and each subgroup can be classified into and 3.Because MT exhibit the selective chelating power for heavy metal ion such as zine(Zn),copper(Cu),cadmium(Cd)and platinum(Pt),they are involved in heavy metal complex.MT has close relation to absorption，transportation and metabolism of many trace elements.MT has also been implicated in chemoresistance to anticancer drugs and in scavenging free radical in cells4.The clearance ability of MT is 38.5 times higher than that of glutathione according to the relevant report,e