各種激發(fā)光和吸收光波長(zhǎng)

1、各種激發(fā)光和吸收光波長(zhǎng)現(xiàn)在發(fā)現(xiàn)一個(gè)更全的有關(guān)激發(fā)光和發(fā)射光的波長(zhǎng)圖譜。這對(duì)CONFOCA法做多重染色時(shí)是必不可少的參考資料。全部文件Excitation and Emission Wavelengths of FluorophoresTavi's "FluoroTable": Common fluorophores (from )FluorophoreAbsorptionEmissionOther info1,5 IAEDANS3364901,8-ANS3724804-Methylumbelliferone3855025-carboxy-2,7-dichlorofl

2、uorescein5045295-Carboxyfluorescein (5-FAM)4925185-Carboxynapthofluorescein (pH 10)512/598563/668Ratio Dye, pH5-Carboxytetramethylrhodamine (5-TAMRA)5425685-FAM (5-Carboxyfluorescein)4925185-HAT (Hydroxy Tryptamine)370-415520-5405-Hydroxy Tryptamine (HAT)370-415520-5405-ROX (carboxy-X-rhodamine)5786

3、045675915-TAMRA (5-Carboxytetramethylrhodamine)5485525425686-Carboxyrhodamine 6G5185436-CR 6G5185436-JOE5205487-Amino-4-methylcoumarin3514307-Aminoactinomycin D (7-AAD)5466477-Hydroxy-4-methylcoumarin360449, 4559-Amino-6-chloro-2-methoxyacridine412, 430471,474ABQ344445Acid Fuchsin540630ACMA (9-Amino

4、-6-chloro-2-methoxyacridine)412, 430471,474Acridine Orange + DNA502526Acridine Orange + RNA460650Acridine Orange, both DNA & RNA440-480520-650Acridine Red455-600560-680Acridine Yellow470550Acriflavin436520Acriflavin Feulgen SITSA355-425460Aequorin (Photoprotein)466PhotoproteinAFPs - AutoFluoresc

5、ent Protein - (QuantumBiotechnologies) see sgGFP sgBFPAlexa Fluor 350346442342441Alexa Fluor 430431540Alexa Fluor 488495, 492519, 520Alexa Fluor 532531,532553, 554Alexa Fluor 546556, 557572,573Alexa Fluor 568577, 578603Alexa Fluor 594590,594617, 618Alexa Fluor 633632650Alexa Fluor 647647666Alexa Flu

6、or 660668698Alexa Fluor 680679702Alizarin Complexon530-560,580,580624-645Alizarin Red530-560580Allophycocyanin (APC)630, 645655, 660AMC, AMCA-S345445AMCA (Aminomethylcoumarin)345425347444AMCA-X353442Aminoactinomycin D555655Aminocoumarin346442350445Aminomethylcoumarin (AMCA)345425347444Anilin Blue600

7、Anthrocyl stearate360-381446APC (Allophycocyanin)630, 645655, 660APC-Cy7625-650755APTRA-BTC = Ratio Dye, Zn2+466/380520/530 Ratio Dye, Zn2+APTS424505Astrazon Brilliant Red 4G500585Astrazon Orange R470540Astrazon Red 6B520595Astrazon Yellow 7 GLL450480Atabrine436490ATTO-TAG CBQCA465560ATTO-TAG FQ4865

8、91Auramine460550Aurophosphine G450580Aurophosphine450-490515BAO 9(Bisaminophenyloxadiazole)365395BCECF (high pH)492, 503520,528BCECF (low pH)482520Berberine Sulphate430550Beta Lactamase409447, 520BFP blue shifted GFP (Y66H)381,382,445, 447,blue shifted GFP (Y66H)Blue Fluorescent Protein383448Blue Fl

9、uorescent ProteinBFP/GFP FRETBimane398490Bisbenzamide360461Bisbenzimide (Hoechst)360461bis-BTC = Ratio Dye, Zn2+455/405529/505Ratio Dye, Zn2+Blancophor FFG390470Blancophor SV370435BOBO-1462481BOBO-3570602Bodipy 492/515490515Bodipy 493/503533549Bodipy 500/510509515Bodipy 505/515502510Bodipy 530/55052

10、8547Bodipy 542/563543563Bodipy 558/568558569Bodipy 564/570564570Bodipy 576/589579590Bodipy 581/591584592Bodipy 630/650-X625642Bodipy 650/665-X647665Bodipy 665/676605676Bodipy Fl504, 505511,513Bodipy FL ATP505514Bodipy Fl-Ceramide505511Bodipy R6G SE528547Bodipy TMR542574Bodipy TMR-X conjugate544573Bo

11、dipy TMR-X, SE544570Bodipy TR589617Bodipy TR ATP591620Bodipy TR-X SE588616BO-PRO-1462481BO-PRO-3544570Brilliant Sulphoflavin FF430520BTC - Ratio Dye Ca2+464/401533/529Ratio Dye Ca2+BTC-5N - atio Dye, Zn2+459/417517/532Ratio Dye, Zn2+Calcein494517Calcein Blue373440Calcium Crimson588, 589611,615Calciu

12、m Green501,506531Calcium Green-1 Ca2+ Dye506531Ca2+ DyeCalcium Green-2 Ca2+506/503536Ca2+Calcium Green-5N Ca2+506532Ca2+Calcium Green-C18 Ca2+Calcium Orange509549530575576Ca2+Calcofluor WhiteCarboxy-X-rhodamine (5-ROX)Cascade BlueCascade YellowCatecholamineCCF2 (GeneBlazer)CFDA385,405576377398399399

13、400410494395,437,445601420423550552470520440,CFP - Cyan Fluorescent ProteinCFP/YFP FRETChlorophyllChromomycin AChromomycin A430,433,474,475,CyanFluorescentProtein436, (453) 476, (501)480650436-460470445575CL-NERF (Ratio Dye, pH) CMFDA504/514494540520Ratio Dye, pHCoelenterazine Ca2+ Dye, bioluminesce

14、nce(429)465Ca2+ bioluminescence, native moleculeDye,Coelenterazine cp (Ca2+ Dye,)(430)442Ca2+bioluminescenceDye,Coelenterazine f(437)473Ca2+bioluminescenceDye,Coelenterazine fcp452Ca2+bioluminescenceDye,Coelenterazine h(437)464Ca2+bioluminescenceDye,Coelenterazine hcp(433)444Ca2+bioluminescenceDye,C

15、oelenterazine ip441Ca2+bioluminescenceDye,Coelenterazine nCoelenterazine O Coumarin Phalloidin C-phycocyanine(431)460387467575470Ca2+bioluminescenceDye,CPM Methylcoumarin384469MethylcoumarinCTC400-450602CTC FormazanCy2489506Cy3.1 8554568Cy3.5581598Cy3514566552570554Cy5.1 8649666Cy5.5675695Cy56496666

16、70Cy7710, 743767, 805Cyan GFP433 (453)475 (501)cyclic AMP Fluorosensor (FiCRhR)500517CyQuant Cell Proliferation Assay480520Cell Proliferation AssayDabcyl453Dansyl340578Dansyl Amine337517Dansyl Cadaverine335518Dansyl Chloride372518Dansyl DHPE336517Dansyl fluoride356noneDAPI359461Dapoxyl403580Dapoxyl

17、2374574Dapoxyl 3373574DCFDA504529DCFH (Dichlorodihydrofluorescein Diacetate)505535DDAO463607DHR (Dihydorhodamine 123)505534Di-4-ANEPPS496705Di-8-ANEPPS (non-ratio)488605498713DiA (4-Di-16-ASP)456591Dichlorodihydrofluorescein Diacetate (DCFH)505535DiD - Lipophilic Tracer644665Lipophilic TracerDiD (Di

18、IC18(5)644665DIDS341415Dihydorhodamine 123 (DHR)505535DiI (DiIC18(3)549,551565Dinitrophenol349DiO (DiOC18(3)484, 487501,502DiR748780Lipophilic TracerDiR (DiIC18)750779DM-NERF (high pH) DNP497/510349540Ratio Dye, pHDopamine340490-520DsRed558583Red fluorescent proteinDTAF494520DY-630-NHS621660Hemicyan

19、elabel for proteins and DNADY-635-NHS634664Hemicyanelabel for proteins and DNAEBFP383447EnhancedBlueFluorescent ProteinECFP436474EnhancedCyanFluorescent ProteinEGFP488, 498507, 516EnhancedGreenFluorescent ProteinELF 97345530Eosin524545Erythrosin529, 532554,555Erythrosin ITC529555Ethidium Bromide510,

20、 523595, 605Ethidium homodimer-1 (EthD-1)528617EuchrysinEukoLightEuropium (III) chloride430540EYFP513, 520527, 532EnhancedYellowFluorescent ProteinFast Blue360440FDA494520Feulgen (Pararosaniline)570625FIF (Formaldehyd Induced Fluorescence)405433FITC490, 494520, 525FITC Antibody493517Flazo Orange375-

21、530612Fluo-3480506,520, 527506Fluo-4494516Fluorescein (FITC)490, 494520, 525Fluorescein Diacetate494520Fluoro-Emerald495524Fluoro-Gold (Hydroxystilbamidine)361536Fluor-Ruby555582FluorX494520FM 1-43479598FM 4-46515640Fura Red(high pH)572657Fura RedFluo-3Fura-2, high calcium335505Excitation ratio dyeF

22、ura-2, low calcium363512Excitation ratio dyeFura-2/BCECFGenacryl Brilliant Red B520590Genacryl Brilliant Yellow 10GF430485Genacryl Pink 3G470583Genacryl Yellow 5GF430475GeneBlazer (CCF2)GFP (S65T)498516GFP red shifted (rsGFP)498516GFP wild type, non-UV excitation (wtGFP)475509GFP wild type, UV excitation (wtGFP)395509GFPuv385508Gloxalic Acid405460Granular Blue355425Haematoporphyrin530-560580Hoechst 33258345487Hoechst 33342347Filter sets installed on the Axiovert 200M:1) BrightfieldDIC:filter cube contai