模擬失重大鼠腸系膜小動脈平滑肌細(xì)胞電壓依賴性鈣離子通道電流的解讀

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5、 February 25, 2005, 57(1: 27-32 10 2+ KV 上調(diào)結(jié)合 L-VDC 的下調(diào)可使 Ca 內(nèi)流減少，這可能也是失重下主要阻力性動脈血 管功能與結(jié)構(gòu)下 調(diào)的電生理學(xué)自體調(diào)節(jié)機制之一。但 L-VDC 電流密 度的降低是 由于通道表達(dá)的降低還是單個通道功能 的改變引起還有待進(jìn)一步闡明。 參考文獻(xiàn) 1 Zhang LF (張立藩， 丫u ZB, Ma J, Mao QW. Peripheral effector mechanism hypothesis on cardiovascular dysfunction after spaceflight. Prog Physi

6、ol Sci 生理科學(xué)進(jìn)展 2001; 32(1:13-17 (Chinese, English abstract. 2 Buckey JC, Lane LD, Levine BD, Watenpaugh DE, Wright SJ, Moore WE, Gaffney FA, Blomqvist CG. Orthostatic in tolera nee after spaceflight. J Appl Physiol 1996; 81(1: 7-18. 3 Looft-Wilson RC,Gisolfi CV. Rat small mese nteric artery fun cti o

7、n after hin dlimb suspe nsion. J Appl Physiol 2000; 88: 11991206. 4 Ma J, Zhang LN, Zhang LF. Vasoco nstrictor resp on sive ness of hi nd body vascular beds is dimini shed in tail-suspe nded rats. J Gravit Physiol 2000; 7:153-154. 5 Delp MD, Collera n PN, Wilkerson MK, McCurdy MR, Muller-Delp J. Str

8、uctural a nd fun cti onal remodeli ng of skeletal muscle microvasculature is in duced by simulated microgravity. Am J Physiol Heart Circ Physiol 2000; 278: 1866-1873. 6 Mao QW, Zha ng LF, Ma J. Observation on arteriolar network structure in soleus of tail-suspended rats. J Gravit Physiol 1997; 4:129

9、-130. 7 8 Zhang LF. Invited review: Vascular adaptation to microgravity: what have we learned? J Appl Physiol 2001; 91: 2415-2430. Somlyo AP, Somlyo AV. Signal transduction and regulation in smooth muscle. Nature 1994; 372: 231-236. 9 Jackson WF. Ion channels and vascular tone. Hypertension 2000; 35

10、:173-178. 10 Fu ZJ 付兆君,Che ng HW, Zha ng LF, Ma J. Cha nges in potassium curre nts of vascular smooth muscle cells isolated from hin dquarter arteries of rats after 4 weeks simulated weightlessness. Acta Physiol Sin 生理學(xué)報 2002; 54: 525530 (Chinese, En glish abstract . 11 Morey-Holton ER,Globus RK. Hi

11、 ndlimb un loadi ng rode nt model: technical aspects. J Appl Physiol 2002; 92:1367-1377. 12 He JQ 何加強,Qu JH, Zheng YF, Wang XF. Characteristics of voltage-depe ndent Ca2+ cha nn els in smooth muscle cell membra ne of visceral resista nee vessel in SHRsp.Acta Biophys Sin 生物物理學(xué)報 1998; 14(3: 418-427 (C

12、hinese, English abstract. 13 Cox RH Rusch NJ . New expressi on profiles of voltagegated ion cha nn els in arteries exposed to high blood pressure. Microcirculation 2002 ； 9: 243-257. 14 Zha ng LF, Zha ng LN, Me ng QJ, Fu ZJ. Research in differe ntial adaptatio ns of vessels to microgravity. 23rd Ann

13、ual Intern ati onal Gravitational Physiology Meeting; 2002 June, Karolinska Institute, Sweden. ESA SP-501. J Gravit Physiol 2002; 9(1: P55-P58. 15 Yuan JX, Rubin LJ. Altered expression and fun cti on of Kv cha nn els in primary pul monary hyperte nsion. In Archer and Rusch (eds. Potassium Chann els in Cardiovascular Biology. New York: Kluwer Academic/ Ple num Publishers, 2001,821836. 16 Fu ZJ, Xie MJ, Zha