上海交通大學(xué)生物化學(xué)試卷(2003)

1、上海交通大學(xué)生物化學(xué)試卷（2003年度）校名_ 系名_ 專業(yè)_姓名_ 學(xué)號(hào)_ 日期_2003 test paper (1)（閉卷部分）(shanghai jiao tong university,1st term of univ. year 2003)subject: biochemistry name _ score _ class _一. explain the terms:（2.5 x 10= 25）1. hyperuricemia 2. ldl receptor 3. pyruvate dehydrogenase complex 4. glucokinase (in liver) 5.

2、 malate-aspartate shuttle 6. prpp 7. substrate cycle 8. “macro coa” 9. urea cycle 10. udp- glucose 2003 test paper (2)（開卷）(shanghai jiao tong university,1st term of univ. year 2003)subject: biochemistry name _ score _ class _二. filling the blanks: (2.5 x 12= 30)1. many important coenzymes, such as_,

3、 _and _, are the derivatives of adp; dndp are synthesized from _ and dtmp is formed by_ of dump; all these facts reflects that_ came before_ in evolution.2. tpp is the derivative of vitamin _, it serves as the prosthetic group of the following enzymes: _, _and_.3. there is a large difference between

4、 the yield from the complete oxidation of triacylglycerols and of carbohydrates (and protein), the reason is _.4. the three pairs of enzymes that catalyze the three resemble steps in -oxidation of fa and citric acid cycle are_ and_, _and_, _and_. 5. the only reactions in mammals that require coenzym

5、e b12 are_ and _ catalyzed by _ and _, respectively.6. oaa has several possible fates: _, _, _ and _.7. in the synthesis of phosphoglycerides磷酸甘油酯 and of glycogen, the activated intermediates are _ and_, both of them are formed from a _ substrate and a _, and then react with a _ group.8. the committ

6、ed step in cholesterol biosynthesis is the synthesis of _ from _which is catalyzed by _.9. the carrier of the activated one-carbon units is_ which consists of three groups: _, _ and_. besides, _ is the major donor of methyl group. the fully oxidized one-carbon unit is _, which is carried by_.10. dis

7、ease and inherited defects of enzyme or functional protein, e.g. pkuphe hydroxylase; drug-induced hemolytic anemia溶血性貧血_; hypercholesterolemia高膽固醇血癥_; pernicious anemia惡性貧血_; maple syrup槭糖漿 urine disease_; lebers optic neuropathy視神經(jīng)癥 _.11. acetyl coa carboxylase from e. coli consists of three subuni

8、ts called _, _ and _.12. there are two types of covalent modification: _ and_in the regulation of enzymatic activity, the typical example of which is_ and _(enzyme), the direct related residue is_ and _, respectively. 三make choice (one or several)：(3 x 8= 24)1. schiff base intermediates are formed i

9、n the reactions catalyzed bya. aldolase b. glycogen phosphorylase c. trp synthetase d. transaldolase e. aminotransferase2. multifunctional (or bifunctional) enzyme occurs ina. pyrimidine synthesis of eukaryotes b. fa synthesis of eukaryotes c. the synthesis and hydrolysis of f-2,6-bp d. the phosphor

10、ylation and dephosphorylation of isocitrate dh e. fa synthesis of e. coli3. prpp participates in the synthesis of the following substancea. pro b. his c. purine d. phe e. pyrimidine4. biotin, as the posthetic group of the following enzymea. acetyl coa carboxylase b. transketolase c. pyruvate carboxy

11、lase d. pyr dh e. kg dh5. the following complex contains fe-s clustera. nadh-q reductase b. cytochrome c reductase c. cytochrome c oxidase d. succinate-q reductase e. ribonucleotide reductase6. which substance is the direct precursor of the synthesis of acetoacetate乙酰 乙酸 in livera. hmg coa b. acetoa

12、cetyl coa c. d-3-hydroxybutyrate羥丁酸 d. d-3-hydroxybutyryl coa e. acetone7. citric acid cycle a. it can produce high-energy phosphoryl compound by itself b. it is not inhibited by the anaerobic厭氧 condition c. the two carbon atoms of acetyl coa are liberated as the form of two co2 in that round of the

13、 cycle d. its rate depends upon the need of atp e. it is the final common pathway for the oxidation of fuel molecules.8. it participates in the activated methyl cycle (or s-a met cycle)a. vb12 b. biotin c. fh4 d. vb2 e. nicotinate煙酸四. answer or calculate: (7x 3= 21)1. what is the activated reactant

14、in each of these biosyntheses?a) sphingomyelin鞘磷脂 from ceramide神經(jīng)酰胺b) met from homocysteine高半胱氨酸c) phosphatidyl choline磷脂酰膽堿 from phosphatidyl ethanolamine乙醇胺d) ipp from 5-pyrophosphomevalonate焦磷酸甲羥戊酸e) the synthesis of phosphoribosylamine磷酸核糖胺f) ser from glyg) glycogen n+1 from glycogen n 2. in pur

15、ine biosynthesis, asp becomes linked to the carboxylate group of an intermediate. in the next reaction, the carbon skeleton of the asp unit leaves in the form of fumurate延胡索酸. these reactions are reminiscent重演 of a similar pair of reactions in the degradation of aa. which ones? 3. currency exchange. for a proton-motive force of