常用緩沖溶液的配制

1、常用緩沖溶液的配制方法1.甘氨酸-鹽酸緩沖液（0.05mol/L ）X毫升0.2 mol/L 甘氨酸+Y毫升0.2 mol/L HCI，再加水稀釋至 200毫升pHXYpHXY2.05044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量=75.07，0.2 mol/L 甘氨酸溶液含15.01克/升。2.鄰苯二甲酸-鹽酸緩沖液（0.05 mol/L )X毫升 0.2mol/L鄰苯二甲酸氫鉀 + 0.2mol/L HCl，再加水稀釋到 20毫升PH(20 C)XYPH(20 C)XY2.254.0703.25

2、1.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.022鄰苯二甲酸氫鉀分子量：=204.23 , 0.2mol/L鄰苯二甲酸氫溶液含40.85克/升3.磷酸氫二鈉-檸檬酸緩沖液pH0.2 mol/L0.1 mol/LpH0.2 mol/L0.1 mol/LNaHPO檸檬酸NaHPQ檸檬酸（毫升）（毫升）（毫升）（毫升）2.20.4010.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04

3、.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55NaHPQ分子量=14.98 , 0.2 mol/L 溶液為 28.40 克/

4、升。NaHPO- 2H2Q 分子量=178.05 , 0.2 mol/L 溶液含 35.01 克/ 升。C4H2Q7 HO分子量=210.14 , 0.1 mol/L 溶液為 21.01 克/ 升。4 .檸檬酸-氫氧化鈉-鹽酸緩沖液pH鈉離子濃度（mol/L ）檸檬酸（克）CHO HaO氫氧化鈉（克）NaOH 97%鹽酸（毫升）HCl （濃）最終體積（刑2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610使用

5、時(shí)可以每升中加入1克克酚，若最后pH值有變化，再用少量50%氫氧化鈉溶液或濃鹽酸調(diào)節(jié)，冰箱保存。5檸檬酸-檸檬酸鈉緩沖液（0.1 mol/L ）pH3.03.23.43.60.1 mol/L檸檬酸（毫升）18617.216.014.90.1 mol/L檸檬酸鈉（毫升）2.84.05.1pH0.1 mol/L檸檬酸（毫升）0.1 mol/L檸檬酸鈉（毫升）3.84.04.214.013.112.36.06.97.74.44.64.811.410.39.28.69.710.85.08.211.85.27.312.75.46.413.65.65.514.55.84.715.36.03.816.26.

6、22.817.26.42.018.06.61.418.6檸檬酸 GHO- HO:分子量210.14 , 0.1 mol/L溶液為21.01克/升。檸檬酸鈉 Na3 C6H5O7 2HO 分子量294.12 , 0.1 mol/L 溶液為29.41克/毫升。6.乙酸-乙酸鈉緩沖液（0.2 mol/L ）PH（18 C）0.2mol/LNaAc（毫升）0.3mol/LHAc（毫升）pH（18 C）0.2mol/LNaAc（毫升）0.3mol/LHAc（毫升）2.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.

7、657.355.48.601.404.43.706.305.69.100.904.64.905.105.89.400.60NaAc 3H2O分子量=136.09 , 0.2 mol/L 溶液為 27.22 克/ 升。7.磷酸鹽緩沖液（1）磷酸氫二鈉-磷酸二氫鈉緩沖液（0.2 ）pH5.85.96.06.10.2 mol/LNa2 HPO（毫升）810.012.315.00.3 mol/LNaH2 PQ（毫升）92B90.087.785.0pH0.2 mol/LNaHPO（毫升）0.3 mol/LNaH2 PO（毫升）6.26.318.522.581.577.56.46.56.66.76.86.

8、926.531.537.543.549.555.073.568.562.556.551.045.07.061.039.07.167.033.07.272.028.07.377.023.07.481.019.07.584.016.07.687.013.07.789.510.57.891.58.57.993.07.08.094.75.3NaHPO- 20分子量=178.05 , 0.2 mol/L 溶液為 85.61 克/升。NaHPO 12H2O分子量=358.22 , 0.2 mol/L 溶液為 71.64 克/ 升。pHmol/L/15Na 2HPO（毫升）mol/L/15KH 2PQ（毫升

9、）pHmol/L/15Na 2HPO（毫升）mol/L/15K（毫升4.920.109.907.17.003.005.290.509.5078.002.005.911.009.007.39.001.006.242.008.0089.500.506.473.007.007.79.750.256.644.006.0039.900.106.816.985.006.005.004.008.048.348.678.1810.000NaHPO- 2H2O分子量=156.03 , 0.2 mol/L 溶液為 31.21 克/升。（2）磷酸氫二鈉-磷酸二氫鉀緩沖液（1/15 mol/L ）H 2PQ）NaHP

10、O- 2H2O分子量=178.05 ，1/15M 溶液為 11.876 克/升。KHPQ分子量=136.09 , 1/15M溶液為9.078克/升。&磷酸二氫鉀-氫氧化鈉緩沖液（0.05M）X毫升0.2M K 2PO + Y毫升0.2N NaOH加水稀釋至29毫升pH（20 C）X （毫升）Y （毫升）pH（20 C）X （毫升）Y （毫升）5.850.3727.052.9636.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.852.3658.054.680溶液為8.25克/升10. Tris -

11、鹽酸緩沖液（0.05M,50毫升0.1M三羥甲基氨基甲烷（ 毫升。9.巴比妥鈉-鹽酸緩沖液（18C）pH0.04M巴比妥鈉溶液0.2V鹽酸pH0.04M巴比妥 鈉溶液（毫 升）0.2N 鹽酸 （毫升）6.810018.48.41005.217.010017.88.61003.827.210016.78.81002.527.410015.39.01001.657.610013.49.21001.137.810011.479.41000.708.01009.399.61000.358.21007.21100巴比妥鈉鹽分子量=206.18;0.04M25C）Tris ）溶液與X毫升0.1N鹽酸混勻后

12、，加水稀釋至100pHX（毫升）pHX（毫升7.1045.78.1026.27.2044.78.2022.97.3043.48.3019.97.4042.08.4017.27.5040.38.5014.77.6038.58.6012.47.7036.68.7010.37.8034.58.808.57.9032.08.907.08.0029.2)三羥甲基氨基甲烷（Tris）HOCH2 CH2OHCH0CH2 NH2分子量=121.14;0. 1M溶液為12.114克/升。Tris溶液可從空氣中吸收二氧化碳，使用時(shí)注意將瓶蓋嚴(yán)。11 .硼酸-硼砂緩沖液（0.2M硼酸根）pH0.05M硼砂（毫升）0

13、.2M硼砂（毫 升）pH0.05M硼砂（毫升）0.2M硼酸（毫升）7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂NaBOrHO,分子量=381.43;0.05M 溶液（=0.2M硼酸根）含19.07克/升。 硼酸H2BO,分子量=61.84,0.2M 溶液為12.37克/升。硼砂易失去結(jié)晶水，必須在帶塞的瓶中保存。11.硼酸-硼砂緩沖液（0.2M硼酸根）pH0.05M硼砂（毫升）0.2M硼酸（毫升）pH0.05M硼砂（毫升）0.2M硼酸（毫升）7.41.09.08.23.56.57.61.58

14、.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂NaBJ 10H2O,分子量=381.43;0.05M 溶液（=0.2M硼酸根）含19.07克/升。硼酸H2BO,分子量=61.84, 0.2M 溶液為12.37克/升。硼砂 易失去結(jié)晶水，必須在帶塞的瓶中保存。12甘氨酸-氫氧化鈉緩沖液（0.05M）X毫升0.2M甘氨酸+Y毫升0.2NnaOH加水稀釋至200毫升pHXYpHXY8.6504.09.65022.48.8506.09.85027.29.0508.810.05032.09.25012.010.45038.69.45016.810.65

15、045.5甘氨酸分子量=75.07;0.2M 溶液含15.01克/升。13.硼砂-氫氧化鈉緩沖液（0.05M硼酸根）X毫升0.05M硼砂+Y毫升0.2NNaOH加水稀釋至200毫升pHXYpHXY9.3506.09.85034.09.45011.010.05043.09.65023.010.15046.0硼砂 Na2B4O 10H2O,分子量=381.43;0.05M 溶液為 19.07 克/ 升。14碳酸鈉-碳酸氫鈉緩沖液（0.1M）CsT、Mg+存在時(shí)不得使用pH0.1MNa2CO（毫升）0.1MN2HCO：毫升）20 C37 C9.168.77199.409.12289.519.4037

16、9.789.50469.909.725510.149.906410.2810.087310.5310.288210.8310.5791NaCO IOH2O分子量=286.2;0.1M 溶液為28.62克/升。N2HC分子量=84.0;0.1M 溶液為8.40克/升。15.“PBS緩沖液PH7.67.47.27.0HO100010001000100mlNaCl8.58.58.58.5gNaH PO2.22.22.22.2gNaH PO0.10.20.30.4g附錄四常用緩沖溶液的配制（一）磷酸氫二鈉-檸檬酸緩沖液pH0.2 mol/LNa2 HPO4 (mL)0.1 mol/L 檸檬酸(mL)p

17、H0.2 mol/LNa2HPO4(mL)0.1檸檬酸2.20.4019.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.23.2811.727.217.392.614.43.8211

18、.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55mol/L(mL)Na2HPO4 分子量=141.98; 0.2 mol/L 溶液為 28.40 g/L。Na2HPO4 - 2H2O 分子量=178.05; 0.2 mol/L 溶液為 35.61 g/L。Na2 HPO4 12H2O 分子量=358.22; 0.2 mol/L 溶液為 71.64 g/L。C6H8O7 - H2O 分子量=210.14; 0.1 mol/L 溶液為 21.01 g/L。二)檸檬酸 - 檸

19、檬酸鈉緩沖液( 0.1 mol/L )pH0.1mol/L0.1 mol/LpH0.1 mol/L0.1 mol/L檸檬酸(mL)檸檬酸鈉（mL）檸檬酸（mL）檸檬酸鈉（mL）3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39.76.61.418.64.89.210.8檸檬酸：C6H8O7 H2O 分子量=210

20、.14 ; 0.1 mol/L 溶液為 21.01 g/L。檸檬酸鈉：Na3C6H5O7 - 2H2O 分子量=294.12 ; 0.1 mol/L 溶液為 29.41 g/L。（三）醋酸-醋酸鈉緩沖液（0.2 mol/L ）pH0.2 mol/L0.2 mol/LpH0.2 mol/L0.2 mol/L(18 C)NaAc (mL)HAc (mL)(18 C)NaAc (mL)HAc (mL)3.60.759.354.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.

21、69.100.904.64.905.105.86.400.60NaAc 3H2O 分子量=136.09; 0.2 mol/L 溶液為 27.22 g/L。 冰乙酸11.8 mL稀釋至1 L (需標(biāo)定)。（四）磷酸二氫鉀-氫氧化鈉緩沖液（0.05 mol/L）X 毫升0.2 mol/L KH2PO4+Y 毫升0.2 mol/L NaOH 加水稀釋至 20毫升。pH (20 C)X (mL)Y (mL)pH (20 C)X (mL)Y (mL)5.850.3727.052.9636.050.5707.253.5006.250.8607.43.9506.451.2607.654.2806.651.7

22、807.854.5206.852.3658.054.680（五）磷酸氫二鈉-磷酸二氫鈉緩沖液（0.2 mol/L ） pH0.2 mol/L0.2 mol/LpH0.2 mol/L|o.2 mol/LNa2 HP04 (mL) N aH2 P04 (mL) |Na2 HP04 (mL) |NaH2 P04 (mL)Na2HP04Na2HP04NaH2P04NaH2P045.88.092.07.061.039.05.910.090.07.167.033.06.012.387.77.272.028.06.115.085.07.377.023.06.218.581.57.481.019.06.322

23、.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.051.08.094.75.36.955.045.0-2H2O 分子量=178.05; 0.2 mol/L 溶液為 35.61 g/L。-12H2O 分子量=358.22 ; 0.2 mol/L 溶液為 71.64 g/L。-H2O 分子量=138.01 ; 0.2 mol/L 溶液為 27.6 g/L。-2H2O 分子量=156.03; 0.2 mol/L 溶液為 31.21

24、g/L。（六）巴比妥納-鹽酸緩沖液pH(18 C)0.04 mol/L 巴比妥鈉(mL)0.2 mol/LHCl (mL)PH(18C)0.04 mol/L巴比妥鈉(mL)0.2 mol/LHCl (mL)6.810018.48.41005.217.010017.88.61003.827.210016.78.81002.527.410015.39.01001.657.610013.49.21001.137.810011.479.41000.708.01009.399.61000.358.21007.21=206.18; 0.04 mol/L 溶液為 8.25 g/L。緩沖液(0.05 mol/

25、L )巴比妥鈉分子量一（七） Tris-HCl50毫升O.1mol/L三羥甲基氨基甲烷（Tris）溶液與X毫升O.1mol/L鹽酸混勻并稀釋至100毫升。pH (25 C)X (mL)pH (25 C)X (m7.1045.78.1026.27.2044.78.2022.97.3043.48.3019.97.4042.08.4017.27.5040.38.5014.77.6038.58.6012.47.7036.68.7010.37.8034.58.808.57.9032.08.907.08.0029.2Tris分子量=121.14 ; 0.1 mol/L溶液為12.114 g/L。Tris溶液可從空氣中吸收二氧