同尾酶匯總表

1、 連接互相匹配的粘性末端，產(chǎn)生新的酶切位點 通常連接互相匹配的粘性末端可以產(chǎn)生新的酶切位點。以下組合是通過計算機程序計算出來的，盡管我們希望能盡量保證其準(zhǔn)確性， 但有些未經(jīng)實驗證實，因此不能保證 100%勺可行性。如果有同裂酶存在，貝U只列出其中的一個酶。本表中列出的內(nèi)切酶均為已商品化 的內(nèi)切酶。 識別序列不夠嚴(yán)謹(jǐn)?shù)膬?nèi)切酶（例如，識別多個序列的酶）只在括號內(nèi)標(biāo)出相關(guān)的序列，但注意這些酶不只切割該序列。 -表示連接產(chǎn)物不能被再切割。 酶 連接到 可進行切割的酶 Acc65l (G/GTACC) Ban l(G/GTACC) Acc65I,Ba nl,Kp nl,NlalV,Rsal BsiWI,

2、BsrGI Rsal Accl (GT/CGAC) Acil,Acll,BsaHI(GR/CGRC),Hi nP1l,Hpall,Narl - (GT/CGAC) Clal,BstBI,Taql Taql Acil (C/CGC) Accl(GT/CGAC),Acll,Clal,BstBI,Taql - BsaHI(GR/CGCC),Hi nP1I,NarI Acil| Hpall Hpall Acll (AA/CGTT) Accl(GT/CGAC),Acil,Clal,BstBI,Hi nP1l,Hpall,Narl,Taql - 1 Afllll (A/CGCGT) AscI,BssHII

3、 BstUI,Hhal (A/CATGT) BspHI,NcoI,PciI Nlalll (A/CGCGT) Mlul AflIII,BstUI,MluI Agel (A/CCGGT) Aval(C/CCGGG),Rmal Hpall,Ncil,ScrFI BsaWI,BspEI BsaWI,HpaII BsrFI(A/CCGGT),SgrAI(CA/CCGGTG) AgeI,BsaWI,BsrFI,HpaII NgoMIV BsrFI,HpaII Apal (GGGCC/C) Ba nll(GGGCC/C),Bsp1286l(GGGCC/C) ApaI,Ba nII,Bsp120I,Bsp1

4、286I,HaeIII,NlaIV,Sau96I ApaLI (G/TGCAC) SfcI(C/TGCAG) Bsgl Apol (A/AATTR) EcoRI ApoI,Tsp509I (G/AATTR) EcoRI ApoI,EcoRI,Tsp509I (R/AATTR) Mfel,Tsp509l Tsp509l AscI (GG/CGCGCC) AflIII(A/CGCGT),MluI BstUI,Hhal BssHII BssHII,BstUI,Cac8l,Hhal AseI (AT/TAAT) Bfal,Csp6l,Ndel - Msel Msel AsiSI (GCGAT/CGC)

5、 BsiEI(CGAT/CG) Dp nll,Pvul Pacl Msel Pvul Dp nll,Pvul AvaI (C/CCGGG) Agel,BsaWI,BspEI,BsrFI(R/CCGGR),NgoMIV,SgrAI(CR/CCGGRG) HpaII,NciI,ScrFIj (C/TCGAG) Rhol Aval,Taql,Rhol (C/TCGAG)| Sall Taql (C/CCGGG) Rmal Aval,BsaJI,Hpall,Ncil,ScrFI,Smal AvaII (G/GWCC) PpuMI(RG/GACCR) Avall,NlalV,Sau96l Rsrll A

6、vaII,Sau96I PpuMI(RG/GTCCR) Avall,BsmFI,NlalV,Sau96l Avril Nhel,Spel,Rbal Bfal (C/CTAGG) StRI(C/CTAGG) Avrll,Bfal,BsaJI,StRI BamHI Bcll,Dp nil AlwI,Dp nil (G/GATCC) Bglll,BstRI(R/GATCR) Alwl,BstRI,Dp nil BstRI(G/GATCC) Alwl,BamHI,BstRI,Dp nii,NlaIV 3anl (G/GTACC) Acc65l Acc65i,Ba nl,Kp nl,NlalV,Rsal

7、 (G/GCGCC) Kasl Ba nl,BsaHI,Haell,Hhal,Kasl,Narl,NlalV (G/GTACC) BsiWI,BsrGI Rsal 3anll (GGGCC/C) ApaI,Bsp1286I(GGGCC/C) Apai,Ba nii,Bsp1286i,Haeiii,NlaiV,Sau96i (GAGCT/C) Bsp1286l(GAGCT/C),Sacl Alui,Ba nii,BsiHKAI,Bsp1286i,Saci Bell BamHI,BstRI(R/GATCR) Alwi,Dp nil (T/GATCA) BglII,MboII Dpnll Bfal

8、(C/TAG) Asel,Csp6l,Msel,Ndel - BglllI BamHI,BstRI(R/GATCR) Alwi,BstRI,Dp nil (A/GATCT) BclI,Dp nil Dpnll BsaHI (GR/CGRC) Accl(GT/CGAC),Clal,BstBI,Taql - (GA/CGRC) AciI,Hi nP1l Hgal (GG/CGRC) AciI,Hi nP1l Hhal (GG/CGRC) Hpall Acil (GA/CGRC) Narl BsaHI,Hgai (GG/CGRC) Narl Ba nl,BsaHI,Haell,Hhal,Narl,N

9、lalV BsaWI Agel,BsrFI(R/CCGGR),SgrAI(CR/CCGGRG) AgeI,BsaWI,BsrFI,HpaII (W/CCGGW) Aval(C/CCGGG),Rmal Hpall,Ncil,ScrFI BspEI BsaWI,BspEI,Hpall BsrFI(R/CCGGR),NgoMIV BsrFI,HpaII NgoMIV Hpall BsiEI (CGAT/CG) PacI Msel (CGAT/CG) Pvul BsiEI,Dp nII,PvuI (CGGC/CG) SacII Acil BsiHKAI Bsp1286I(GTGCA/C) BsiHKA

10、I,Bsp1286I (GTGCA/C) Bsp1286I(GAGCA/C) BsiHKAI,Bsp1286I Bsp1286l(GAGCT/C),Sacl AluI,Ba nII,BsiHKAI,Bsp1286I,SacI Nsil - Pstl,Sbfl Bsgl BsiWI (C/GTACG) Acc65I,Ba nl(G/GTACC),BsrGI Rsal 3sp1286l (GGGCC/C) ApaI,Ba nll(GGGCC/C) ApaI,Ba nII,Bsp1286I,HaeIII,NlaIV,Sau96I (GTGCA/C) BsiHKAI ApaLI,BsiHKAI,Bsp

11、1286I (GGGCC/C)| Ban ll(GGGCC/C) Ba nII,Bsp1286I (GAGCT/C) Banl l(GAGCT/C),BsiHKAI,Sacl AluI,Ba nII,BsiHKAI,Bsp1286I,SacI (GWGCW/C) BsiHKAI BsiHKAI,Bsp1286I (GTGCA/C)| Nsil - (GTGCA/C) PstI,SbfI Bsgl BspEI AgeI,BsaWI,BsrFI(R/CCGGR),SgrAI(CR/CCGGRG) BsaWI,HpaII (T/CCGGA) AvaI(C/CCGGG),RmaI HpaII,NciI

12、,ScrFI BsaWI BsaWI,BspEI,Hpall BsrFI(R/CCGGR),NgoMIV Hpall 3spHI (T/CATGA) AflHI(A/CATGT),Ncol,StRI(C/CATGG) Nlalll BsrFI (A/CCGGR) AgeI,BsaWI AgeI,BsaWI,BsrFI,HpaII (G/CCGGR) Agel,BsaWI,NgoMIV BsrFI,HpaII (R/CCGGR) Aval(C/CCGGG),Rmal HpaII,NciI,ScrFI (A/CCGGR) BsaWI,BspEI BsaWI,HpaII (R/CCGGR) BsaW

13、I,BspEI Hpall (G/CCGGR) NgoMIV BsrFI,Cac8l,Hpall,Nael (CR/CCGGRG) BsrGI (T/GTACA) SgrAI BsrFI,HpaII Acc65l,Ba nl(G/GTACC),BsiWI Rsal BssHII (G/CGCGC) AflIII(A/CGCGT),MluI BstUI,Hhal Ascl BssHII,BstUI,Cac8l,Hhal BstBI (TT/CGAA) Accl(GT/CGAC),Clal,Taql Taql| Acil,Acll,BsaHI(GR/CGRC),Hi nP1l,Hpall,Narl

14、 - BstRI (A/GATCR) BamHI,BglII Alwl,BstRI,Dp nil (G/GATCR) BamHI Alwl,BamHI,BstRI,Dp nII,NlaIV (R/GATCR) BclI,Dp nil Dpnll (G/GATCR) BclI,Dp nil AlwI,Dp nil (A/GATCR) Bglll BglII,BstRI,Dp nil ClaI (AT/CGAT) AccI(GT/CGAC),BstBI,TaqI Taql| Acil,Acll,BsaHI(GR/CGRC),Hi nP1I,HpaII,NarI - Dpn ll/Mbol/ Sau

15、3AI(/GATC) BamHI,BstRI(R/GATCC) AlwI,Dp nil Bcll,Bglll,BstRI(R/GATCR) Dpnll Eael (R/GGCCR) PspOMI HaeIII,Sau96I (C/GGCCR) Eagl BsiEI,Eael,Eagl,Haelll (T/GGCCR) Eagl EaeI,HaeIII (C/GGCCR)| Notl Acil,BsiEI,Eael,Eagl,F nu4HI,Haelll (T/GGCCR)| Notl AciI,EaeI,F nu4HI,HaeHI Eagl (C/GGCCG) PspOMI HaeIII,Sa

16、u96I EaeI(R/GGCCR) EaeI,HaeIII EaeI(C/GGCCG) BsiEI,EaeI,EagI,HaeIII Notl AciI,BsiEI,EaeI,EagI,F nu4HI,Haelll EcoRl ApoI(G/AATTC) ApoI,EcoRI,Tsp509I (G/AATTC) ApoI(R/AATTR) ApoI,Tsp509I Mfel,Tsp509l Tsp509l Hi nP1l (G/CGC) Accl(GT/CGAC),Acll,Clal,BstBI,Taql - AciI,BsaHI(GR/CGCC),NarI Hhal BsaHI(GR/CG

17、TC) Hgal Hpall Acil Hpall/Mspl (C/CGG) Accl(GT/CGAC),Acll,Clal,BstBI,Taql - AciI,BsaHI(GR/CGCC),Hi nP1I,NarI Acil Kasl (G/GCGCC) Ba nl (G/GCGCC) Ba nl,BsaHI,Haell,Hhal,Kasl,Narl,NlalV Mfel (C/AATTG) ApoI(R/ATTTR),EcoRI,Tsp509I Tsp509l Mlul AflIII(A/CGCGT) AflIII,BstUI,MluI (A/CGCGT) Ascl,BssHII BstU

18、I,Hhal Msel (T/TAA) Asel Msel Bfal,Csp6l,Ndel - Narl (GG/CGCC) Accl(GT/CGAC),Acll,Clal,BstBI,Taql - AciI,Hi nP1l Hhal BsaHI(GR/CGCC) Ba nl,BsaHI,Haell,Hhal,Narl,NlalV BsaHI(GR/CGTC) BsaHI,HgaI Hpall Acil Ncol (C/CATGG) AflIII(A/CATGT),BspHI Nlalll StRI(C/CATGG) BsaJI,Ncol,Nlalll,StRI Pcil Nlalll Nde

19、l (CA/TATG) Asel,Bfal,Csp6l,Msel - NgoMIV (G/CCGGC) Agel,BsaWI,BsrFI(R/CCGGR),SgrAI BsrFI,HpaII AvaI(C/CCGGG),RmaI HpaII,NciI,ScrFI BsaWI,BspEI Hpall| BsrFI(R/CCGGC),SgrAI BsrFI,Cac8l,Hpall,Nael Nhel (G/CTAGC) Avrll,Spel,StRI(C/CTAGG),Rbal Bfal Nlalll (CATG/) SphI,NspI Nlalll Notl (GC/GGCCGC) PspOMI

20、 AciI,EaeI,F nu4HI,HaeHI Eagl Acil,BsiEI,Eael,Eagl,F nu4HI,Haelll EaeI(R/GGCCR) Acil,BsiEI,Eael,F nu4HI,HaeHI Nsil (ATGCA/T) BsiHKAI(GTGCA/C),Bsp1286I(GTGCA/C),PstI,SbfI Nspl NlaIII,SphI Nlalll (RCATG/R) Pacl (TTAAT/TAA) BsiEI(CGAT/CG),Pvul Msel 叱il (A/CATGT) Aflll(A/CATGT),BspHI,Ncol,StRI(C/CTAGG)

21、Nlalll PpuMI (RG/GWCCR) AvaII,RsrII Avall,Sau96l (GG/GTCCR)| AvaII,RsrII Avall,BsmFI,NlalV,Sau96l (GG/GACCR) AvaII,RsrII AvaII,NlaIV,Sau96I PspOMI (G/GGCCC) EaeI(R/GGCCR),EagI HaeIII,Sau96I Notl AciI,F nu4HI,Haelll,Sau96l Pstl (CTGCA/G) BsiHKAI,Bsp1286I(GTGCA/C) Bsgl| Nsil - Sbfl Pstl Pvul (CGAT/CG)

22、 AsiSI Dpn l,Pvul Pacl Msel BsiEI(CGAT/CG) BsiEI,Dp nII,PvuI Rsrll (CG/GWCCG) AvaII,PpuMI(RG/GACCR) AvaII,Sau96I PpuMI(RG/GACCR) AvaII,NlaIV,Sau96I PpuMI(RG/GTCCR) Avall,BsmFI,NlalV,Sau96l Sacl (GAGCT/C) Ban ll(GAGCT/C),BsiHKAI,Bsp1286l(GAGCT/C) AluI,Ba nII,BsiHKAI,Bsp1286I,SacI Sacll (CCGC/GG) BsiEI