GB/T 17817-2024飼料中維生素A的測(cè)定高效液相色譜法

ICS

65.120

CCS

B46

中華人民共和國國家標(biāo)準(zhǔn)

GB/T17817—2024

代替GB/T17817－2010

飼料中維生素A的測(cè)定高效液相

色譜法

DeterminationofvitaminAinfeeds—Highperformanceliquidchromatography

2024-11-28發(fā)布2025-06-01實(shí)施

國家市場(chǎng)監(jiān)督管理總局發(fā)布

國家標(biāo)準(zhǔn)化管理委員會(huì)

GB/T17817—2024

目次

前言

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Ⅲ

1

范圍

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2

規(guī)范性引用文件

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3

術(shù)語和定義

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4

第一法皂化提取法

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1

4.1

原理

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1

4.2

試劑或材料

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1

4.3

儀器設(shè)備

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2

4.4

樣品

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2

4.5

試驗(yàn)步驟

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3

4.6

試驗(yàn)數(shù)據(jù)處理

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4.7

精密度

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5

第二法直接提取法

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5.1

原理

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5.2

試劑或材料

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7

5.3

儀器設(shè)備

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7

5.4

樣品

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8

5.5

試驗(yàn)步驟

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8

5.6

試驗(yàn)數(shù)據(jù)處理

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8

5.7

精密度

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9

附錄A（規(guī)范性）

維生素A和維生素A乙酸酯標(biāo)準(zhǔn)儲(chǔ)備溶液的質(zhì)量濃度校正方法

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10

附錄B（資料性）

在線固相萃取閥的切換流程圖

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11

附錄C（資料性）

維生素A標(biāo)準(zhǔn)溶液的液相色譜圖

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12

附錄D（資料性）

維生素A乙酸酯標(biāo)準(zhǔn)溶液色譜圖

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14

Ⅰ

GB/T17817—2024

前言

本文件按照GB/T1.1—2020《標(biāo)準(zhǔn)化工作導(dǎo)則第1部分：標(biāo)準(zhǔn)化文件的結(jié)構(gòu)和起草規(guī)則》的規(guī)

定起草。

本文件代替GB/T17817—2010《飼料中維生素A的測(cè)定高效液相色譜法》，與GB/T17817—

2010相比，除結(jié)構(gòu)調(diào)整和編輯性改動(dòng)外，主要技術(shù)變化如下：

a）更改了適用范圍，增加了精料補(bǔ)充料和復(fù)合預(yù)混合飼料，更改了“第二法直接提取法”定量

限（見第1章，2010年版的第1章）；

b）增加了“第一法皂化提取法”中維生素A標(biāo)準(zhǔn)系列溶液（見4.2.19）;

c）將“第一法皂化提取法”中配合飼料、精料補(bǔ)充料、濃縮飼料樣品制備方法更改為試樣全部

通過1mm孔篩（見4.4，2010年版的3.5）；

d）將“第一法皂化提取法”提取劑更改為“石油醚”（沸程30℃～60℃）（見4.5.1.2.1，

2010年版的3.6.1.2）；

e）增加了離線固相萃取法（見4.5.1.2.2）和在線固相萃取法（見4.5.1.2.3）；

f）刪除了正相色譜條件（見2010年版的3.6.2.1.1）；

g）增加了液相色譜參考條件（見4.5.2.2和4.5.2.3）；

h）增加了定性檢測(cè)方法，定量檢測(cè)增加多點(diǎn)校正（見4.5.2.5，4.5.2.6）；

i）更改了“第一法皂化提取法”中試驗(yàn)數(shù)據(jù)處理（見4.6，2010年版的3.6.2.3.1）；

j）在“第二法直接提取法”中增加了維生素A標(biāo)準(zhǔn)系列溶液（見5.2.5）；

k）更改了“第二法直接提取法”提取溫度，由65℃改為室溫（見5.5.1，2010年版的4.6.1）；

l）更改了“第二法直接提取法”中試驗(yàn)數(shù)據(jù)處理（見5.6，2010年版的4.6.2.3）；

m）增加了校正維生素A及維生素A乙酸酯標(biāo)準(zhǔn)儲(chǔ)備溶液質(zhì)量濃度的方法（見附錄A）；

請(qǐng)注意本文件的某些內(nèi)容可能涉及專利。本文件的發(fā)布機(jī)構(gòu)不承擔(dān)識(shí)別專利的責(zé)任。

本文件由全國飼料工業(yè)標(biāo)準(zhǔn)化技術(shù)委員會(huì)（SAC/TC76）提出并歸口。

本文件起草單位：中國農(nóng)業(yè)科學(xué)院農(nóng)業(yè)質(zhì)量標(biāo)準(zhǔn)與檢測(cè)技術(shù)研究所、山東省畜產(chǎn)品質(zhì)量安全中心、

帝斯曼維生素（上海）有限公司、四川威爾檢測(cè)技術(shù)股份有限公司、中牧實(shí)業(yè)股份有限公司、廣州愛保

農(nóng)生物科技有限公司。

本文件主要起草人：趙小陽、宋榮、虞哲高、張鳳枰、劉向陽、朱高群、劉志英、郭紅雙、汪忠艷、

張瑋、謝麗、李麗蓓、張輝、宋艷、馮秀燕、曹林。

本文件及其所代替文件的歷次版本發(fā)布情況為：

—1999年首次發(fā)布為GB/T17817—1999，2010年第一次修訂；

—本次為第二次修訂。

Ⅲ

GB/T17817—2024

飼料中維生素A的測(cè)定高效液相

色譜法

1范圍

本文件描述了飼料中維生素A的高效液相色譜測(cè)定方法。

本文件中“第一法皂化提取法”適用于配合飼料、精料補(bǔ)充料、濃縮飼料、復(fù)合預(yù)混合飼料、維

生素預(yù)混合飼料中維生素A的測(cè)定；“第二法直接提取法”適用于復(fù)合預(yù)混合飼料、維生素預(yù)混合飼

料中維生素A乙酸酯的測(cè)定。

本文件第一法定量限為1000IU/kg，第二法定量限為20000IU/kg。

2規(guī)范性引用文件

下列文件中的內(nèi)容通過文中的規(guī)范性引用而構(gòu)成本文件必不可