DB32T 4056-2021 優(yōu)良食味半糯粳稻品質(zhì)

DB32Qualityofsemi-glutinousjaponicaricewithgoodtaste江蘇省市場監(jiān)督管理局發(fā)布I宋錦花、魏海燕、趙春芳、魏曉東、陳濤、趙慶勇、趙凌、姚姝、周麗慧、路凱、梁文12規(guī)范性引用文件GB/T5491糧食、油料檢驗(yàn)扦樣GB/T21719稻谷整精米GB/T22294糧油檢驗(yàn)大米膠稠度NY/T593食用稻品種品質(zhì)NY/T2639稻米直鏈淀粉的測定分光光度法半糯粳稻semi-glutinousjaponicarice優(yōu)良食味半糯粳稻semi-glutinousjaponicaricewithgoodtaste2鑒別標(biāo)記identificationmolecular4質(zhì)量要求4.1基本質(zhì)量要求4.2定等質(zhì)量指標(biāo)一二三整精米率/%123膠稠度/mm5.5透明度檢驗(yàn)：按照NY/T831DB32/T4056-2021A.1原理2A.3.4DNA回收試劑A.5分子標(biāo)記序列正向外引物（Wxmq/Wxmp-O-F）序列為5A.5.2用于鑒定Wxmw的分子標(biāo)記序列3A.8.1瓊脂糖凝膠制備A.8.2上樣樣品制備A.8.4凝膠成像將電泳后的瓊脂糖凝膠放于凝膠成像系統(tǒng)的紫外光源下進(jìn)行PCR擴(kuò)增產(chǎn)物觀察，拍照備用。對引物Wxmq-F/R擴(kuò)增得到的PCR條A.9結(jié)果分析A.9.1分子標(biāo)記檢測結(jié)果分析利用四引物Wxmq/Wxmp-O-F/O-R/I-F/I-R分4和301bpDNA條帶的樣品，證明含Wx"突變位點(diǎn)；擴(kuò)增得到459b 第5外顯子1:GTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGAIACAAAGACAACCAGATGCGTITCAGCCTTCTTT2:GTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGATCACAAAGACAACCAGATGCGTTTCAGCCTTCTTTGC3:GTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGAITACAAAGACAACCAGATGCGTTTCAGCCTTCTTTGCCAG4:GTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGAIACAAAGACAACCAGATGCGTTTCAGCCTTCTTTGCCAG5:GTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGAIACAAAGACAACCAGATGCGTITCAGCCTTCTTTGCCAG6:GTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGATACAAAGACAACCAGATGCGTTTCAGCCTTCTTTGCCAG7:GTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGAIACAAAGACAACCAGATGCGTTTCAGCCTTCTTTGCCAG8:GTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGATACAAAGACAACCAGATGCGTTTCAGCCTTCTTTGCCAG9:GTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGATTACAAAGACAACCAGATGCGTTTCAGCCTTCTTTGCCAG10:GTITGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGAITTACAAAGACAACCAGATGCGTTTCAGCCTTCTTTGCCAG11:GTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGATT/dAACAAAGACAACCAGATGCGTTTCAGCCTTCTTTGCCAG12:GTTTGGGGAAAGACCGGTGAGAAGATCTACCGACCTGACACTGGAGTTOATticAC