細(xì)胞因子：干細(xì)胞治療研究的關(guān)鍵試劑

細(xì)胞因子：干細(xì)胞治療研究的關(guān)鍵試劑前言今年4月份，首個(gè)針對(duì)血液腫瘤的同種異體干細(xì)胞移植療法(Omisirge（omidubicel-onlv）)被FDA批準(zhǔn)，這種療法很好的解決了無(wú)法找到匹配捐贈(zèng)者患者的需求。干細(xì)胞（Stemcell）具有自我更新且分化成多種細(xì)胞的能力，廣泛用于組織修復(fù)、疾病治療、新藥研發(fā)等領(lǐng)域。細(xì)胞因子作為干細(xì)胞培養(yǎng)基的重要添加成分之一，對(duì)干細(xì)胞的增殖和分化等起到重要作用。01干細(xì)胞治療概述干細(xì)胞是一類具有自我更新、自我復(fù)制、向多種細(xì)胞系分化能力的多潛能細(xì)胞，被廣泛用于抗衰老、組織器官的修復(fù)和再生、胚胎發(fā)育、疾病治療和研究、新藥篩選等近乎所有醫(yī)學(xué)范疇。干細(xì)胞的研究（特別是多能干細(xì)胞（PSCs）和間充質(zhì)干細(xì)胞（MSCs））開辟了再生醫(yī)學(xué)的新途徑。隨著骨髓(BM)、脂肪組織(AT)、臍帶(UC)來(lái)源的間充質(zhì)干細(xì)胞(MSCs)的治療潛力被發(fā)現(xiàn)，干細(xì)胞治療的臨床應(yīng)用也在擴(kuò)大。BM-MSCs可作為潛在的腦部、脊髓損傷治療，以及皮膚傷口修復(fù)。UC-MSCs作為潛在的治療方法治療肺部疾病和急性呼吸窘迫綜合癥，而脂肪源間充質(zhì)干細(xì)胞（ASC）可能在將來(lái)用于皮膚再生和治療生殖障礙。02干細(xì)胞增殖及分化干細(xì)胞的增殖和分化由一系列的蛋白和細(xì)胞因子通過內(nèi)源性和外源性的雙重調(diào)控決定的。不同類型的干細(xì)胞被許多細(xì)胞因子靶向，這些細(xì)胞因子改變干細(xì)胞的增殖、分化或其他特性。細(xì)胞因子作為干細(xì)胞培養(yǎng)基的添加成分，對(duì)干細(xì)胞的培養(yǎng)、增殖和分化起著重要作用。細(xì)胞因子調(diào)節(jié)干細(xì)胞增殖、自我更新及分化因子對(duì)干細(xì)胞的主要影響受影響的干細(xì)胞類型調(diào)節(jié)機(jī)制\o"/category/scf/proteins"SCF干細(xì)胞研究主要調(diào)節(jié)因子，與大多數(shù)細(xì)胞因子共同發(fā)揮協(xié)同或拮抗作用，細(xì)胞增殖和自我更新大多數(shù)類型c-Kit信號(hào)通路\o"/category/lif/proteins"LIF促進(jìn)細(xì)胞增殖，促進(jìn)向神經(jīng)元細(xì)胞分化，防止ESCs凋亡大多數(shù)類型STAT3和MEK的激活I(lǐng)L-1加速生長(zhǎng)HSCs轉(zhuǎn)錄因子Pu.1的激活\o"/category/il-1-alpha-il1a/proteins"IL-1α增強(qiáng)骨髓MSCs的神經(jīng)元發(fā)生NSCs、MSCs尚未報(bào)道\o"/category/il-1-beta/proteins"IL-1βMSCs分化為成骨細(xì)胞MSCsMAPK和NF-Kb的激活\o"/category/il2/proteins"IL-2干細(xì)胞重建HSCs尚未報(bào)道\o"/category/il3/proteins"IL-3增殖和存活大多數(shù)類型IL-3信號(hào)通路\o"/category/il-4/proteins"IL-4在早期骨髓生成過程中非常重要，使ESCs分化為成纖維細(xì)胞樣細(xì)胞，促進(jìn)向心肌和骨骼肌細(xì)胞的分化大多數(shù)類型STAT3的激活\o"/category/il-6/proteins"IL-6神經(jīng)前體的調(diào)節(jié)因子，與SCF協(xié)同促進(jìn)造血祖細(xì)胞擴(kuò)增，對(duì)巨核系祖細(xì)胞的增殖也有重要作用，抑制骨髓MSCs向軟骨細(xì)胞分化大多數(shù)類型JAK/STAT信號(hào)通路\o"/category/il-7/proteins"IL-7T細(xì)胞和B細(xì)胞的分化HSCs、ESCsIL-7信號(hào)通路\o"/category/il-8-cxcl8/proteins"IL-8對(duì)造血和神經(jīng)生成都很重要HSCs、NSCs誘導(dǎo)PI3K/Akt和MAPK/ERK介導(dǎo)的VEGF產(chǎn)生\o"/category/il-9#Protein"IL-9原始人紅細(xì)胞的增殖，與SCF、C-Kit和其他細(xì)胞因子協(xié)同促進(jìn)造血祖細(xì)胞的生長(zhǎng)HSCsSTAT3的磷酸化\o"/category/il-10/proteins"IL-10增強(qiáng)繁殖能力，增強(qiáng)再生能力，c-Kit細(xì)胞中添加IL-10后獲得3-4倍的HSCHSCs尚未報(bào)道\o"/category/il11#Protein"IL-11當(dāng)與SCF結(jié)合時(shí)，引起小鼠造血祖細(xì)胞的擴(kuò)增，對(duì)巨核系祖細(xì)胞的增殖也有重要作用HSCs尚未報(bào)道\o"/category/il-13/proteins"IL-13在早期骨髓生成過程中非常重要HSCsIL-13信號(hào)通路\o"/category/il-15/proteins"IL-15從原始前體產(chǎn)生人類記憶干T細(xì)胞HSCsIL-15信號(hào)通路\o"/category/il-16#Protein"IL-16CD34+造血細(xì)胞向成熟DC的增殖和分化HSCsIL-16信號(hào)通路\o"/category/il-27#Protein"IL-27直接或協(xié)同SCF誘導(dǎo)HSCs分化HSCsIL-27信號(hào)通路\o"/category/il-32"IL-32誘導(dǎo)造血祖細(xì)胞增殖，降低化療所致的骨髓毒性HSCsIL-32信號(hào)通路\o"/category/il-33"IL-33促進(jìn)HSCs和祖細(xì)胞的骨髓生成和髓系細(xì)胞遷移HSCs尚未報(bào)道\o"/category/il-34"IL-34對(duì)Langerhans細(xì)胞的發(fā)育必不可少HSCsIL-34信號(hào)通路\o"/category/tnf-alpha/proteins"TNF-α正常HSC活性的有效抑制劑，與IFN-γ協(xié)同消除MSCs的自我更新和分化HSCsTNF受體p55信號(hào)通路\o"/category/interferon-alpha-1/proteins"IFN-αHSCs、NSCs的激活HSCs、NSCsSTAT1和AKT1磷酸化增加\o"/category/tgf-beta-1/proteins"TGF-β調(diào)節(jié)胚胎干細(xì)胞的異質(zhì)性HSCsNodal信號(hào)通路HSCs:HematopoieticStemCells：造血干細(xì)胞；MSCs:MesenchymalStemCells：間充質(zhì)干細(xì)胞；ESCs:EmbryonicStemCells：胚胎干細(xì)胞；NSCs：NeuralStemCells：神經(jīng)干細(xì)胞。Cytokineregulationofstemcells,DOI:10.7287/peerj.preprints.2347v103在研究中的應(yīng)用細(xì)胞因子作為干細(xì)胞培養(yǎng)基的添加成分，對(duì)干細(xì)胞的培養(yǎng)、增殖和分化起著重要作用。Zluhan等在誘導(dǎo)人胚胎干細(xì)胞分化成單核細(xì)胞的時(shí)候，不同階段加入了不同的細(xì)胞因子。比如在spinmedia中添加了50ng/mLVEGF（貨號(hào)：11066-HNAB，義翹神州）和20ng/mLSCF（貨號(hào)：10451-H08B，義翹神州），在mononuclearcellmedia中添加25ng/mLIL-3（貨號(hào)：11858-HNAE，義翹神州）和100ng/mLM-CSF（貨號(hào)：11792-HNAH，義翹神州），表現(xiàn)出良好的細(xì)胞分化。義翹神州細(xì)胞因子產(chǎn)品助力文章發(fā)表貨號(hào)靶點(diǎn)作用文章\o"/recombinant-proteins/human-activin-a-10429-hnah"10429-HNAHINHBA干細(xì)胞分化Shanetal.201710008-HNABVEGF121\o"/recombinant-proteins/human-fgf2-10014-hnae"10014-HNAEFGF211066-HNABVEGF165干細(xì)胞分化Zluhanetal.2016\o"/recombinant-proteins/human-scf-10451-h08b"10451-H08BSCF\o"/recombinant-proteins/human-il3-11858-hnae"11858-HNAEIL3\o"/recombinant-proteins/human-m-csf-11792-hnah"11792-HNAHM-CSF\o"/recombinant-proteins/human-lif-14890-hnah"14890-HNAHLIF干細(xì)胞培養(yǎng)Xuetal.2022?義翹神州細(xì)胞因子產(chǎn)品義翹神州的產(chǎn)品已支持多篇文章發(fā)表在國(guó)內(nèi)外學(xué)術(shù)期刊，涉及到的產(chǎn)品有重組蛋白、抗體、ELISA試劑、基因等。HumanEGFProtein(ECD,HPLC-verified)(Cat:10605-HNAE)高純度Purity:>95%asdeterminedbySEC-HPLC高批間一致性CellproliferationassayusingBalb/C3T3mouseembryonicfibroblasts.HumanOSMProtein(HPLC-verified)(Cat:GMP-10452-HNAH)高批間一致性CellproliferationassayusingTF-1humanerythroleukemiccells.Thespecificactivityis>30,000Units/μgHumanIL3Protein(HPLC-verified)(Cat:GMP-11858-HNAE)高批間一致性CellproliferationassayusingTF-1humanerythroleukemiccells.更多熱門人屬細(xì)胞因子產(chǎn)品貨號(hào)因子內(nèi)毒素純度\o"/recombinant-proteins/human-activin-a-10429-hnah"10429-HNAHINHBA<10≥95%☆,Active\o"/recombinant-proteins/human-oncostatin-m-osm-10452-hnah"10452-HNAHOSM<10≥95%☆,Active\o"/recombinant-proteins/human-egf-10605-hnae"10605-HNAEEGF<5≥95%☆,Active\o"/recombinant-proteins/human-vegfa-11066-hnah"11066-HNAHVEGF165<10≥95%☆,Active\o"/recombinant-proteins/human-il3-11858-hnae"11858-HNAEIL-3<5≥95%☆,Active\o"/recombinant-proteins/human-lif-14890-hnah"14890-HNAHLIF<10≥95%☆,Active\o"/recombinant-proteins/human-fgf2-gmp-10014-hnae"GMP-10014-HNAEFGF2<10≥95%,Active\o"/recombinant-proteins/human-mouse-rat-cynomolgus-rhesus-activin-a-gmp-10429-hnah"GMP-10429-HNAHINHBA<10≥95%☆,Active\o"/recombinant-proteins/human-oncostatin-m-osm-gmp-10452-hnah"GMP-10452-HNAHOSM<5≥95%☆,Active\o"/recombinant-proteins/human-egf-gmp-10605-hnae"GMP-10605-HNAEEGF<5≥95%☆,Active\o"/recombinant-proteins/human-il3-gmp-11858-hnae"GMP-11858-HNAEIL-3<5≥95%☆,Active注：內(nèi)毒素單位：EU/mg，☆表示SEC-HPLC檢測(cè)純度【參考文獻(xiàn)】1.W.J.Zakrzewski,etal.Stemcells:past,present,andfuture.StemCellResearch&Therapy,2019,doi:10.1186/s13287-019-1165-5.2.M.L.Y.Wang,MechanismofMSCsDifferentiationintoHepatocyte-LikeCells:TheRoleofCytokinesandChemicalCompounds.JournalofStemCellResearch&Therapy,2014,doi:10.4172/2157-7633.1000179.3.H.Li,etal.EGF/bFGFpromotessurvival,migrationanddifferentia