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NematodeExtractionMethods:

BaermannFunnelandModifiedBaermannTechniques

KavithaGovindasamyJimmyR.RichMariaL.MendesAPlant-ParasiticNematodeSamplingforNematodesSamplingandextractingofnematodesservestwopurposesDiagnosisacurrentproblemPredictafutureproblem

ObjectivesofsamplingandextractionNematodeidentificationPopulationestimationDiagnoseanematodediseaseMakemanagementdecisionsRegulatorypurposesResearchandsurveysSomeCommonNematode

ExtractionMethodsSoilsamples

BaermannfunnelModifiedBaermannfunnelRootSamplesJarincubationandsievingBlendingandsievingFoliarsamples

PunchingandincubatingScissorstechnique

SoilExtractionTechniques:

BaermannFunnel

PrincipleExtractionbasedontheactivemovementofthenematodes(motilitydependentmethod)MaterialsrequiredFunnelFunnelstandWiremeshTissuepaper/coffeefilterRubberorplastictubeClamporspringclipBeakerorvialPlasticdrinkcupcoverBaermannFunnelsetupSoilsampleoverwiremeshFunnelBeakerClampRubbertubingClamptoholdfunnelBeginBaermannfunnelsetupAddthesoilsampletothecoffeefilterortissuepaperPlacethesampleonthefunnelCoverthetissueoverthesoilIncubatefor48hoursandcollectthenematodesCollectnematodesinabeakerAdvantagesandLimitationsAdvantagesRecoveryofclean,livesamplesSievesnotneededInexpensivetoconstructLimitationsNoteffectiveforinactivenematodesRecoverypercentagesmaybelowModifiedBaermannFunnel

Baermanntrays

(Whitehead&Hemming’smodification,1964)PrincipleExtractionbasedontheactivemigrationofthenematodes(motilitydependentmethod)MaterialsrequiredPanortrayWiremeshTissuepaper,coffeefilterSqueezebottle500meshsieveAddwatertothedishPlacesoilontissuepaperPlacetissuepaperonthewiremeshFoldthetissueoversoil,addwater

Completedtechnique,readyforincubationIncubateatroomtemperaturefor3daysFollowingincubation,removewiremeshfromthedishPourwaterintosieveRinsenematodesfromthesieveAdvantagesandLimitationsAdvantagesRecoveryofclean,livesamplesInexpensivetoconstructMaterialsreadilyavailableLimitationsFinesoilparticlescloudsampleLackofaerationcanbeaproblemSievesareneededtoconcentratenematodesAcknowledgmentsJaneteBrito,DepartmentofPlantIndustry,Gainesville,FloridaFrankWoods,NematodeAssayLab,UniversityofFlorida,Gainesville,FloridaJoeyOrajay,EntomologyandNematologyDepartment

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