GB/T 44428-2024化妝品中大麻二酚和四氫大麻酚的測(cè)定液相色譜-串聯(lián)質(zhì)譜法

ICS

71.100.70

CCS

Y42

中華人民共和國(guó)國(guó)家標(biāo)準(zhǔn)

GB/T44428—2024

化妝品中大麻二酚和四氫大麻酚的測(cè)定

液相色譜?串聯(lián)質(zhì)譜法

Determinationofcannabidiolandtetrahydrocannabinolincosmetics—

Liquidchromatographytandemmassspectrometry

2024-08-23發(fā)布2025-03-01實(shí)施

國(guó)家市場(chǎng)監(jiān)督管理總局發(fā)布

國(guó)家標(biāo)準(zhǔn)化管理委員會(huì)

GB/T44428—2024

目次

前言

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Ⅲ

引言

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Ⅳ

1

范圍

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1

2

規(guī)范性引用文件

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1

3

術(shù)語(yǔ)和定義

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1

4

原理

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1

5

試劑和材料

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1

6

儀器設(shè)備

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2

7

試樣制備和試驗(yàn)步驟

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2

7.1

試樣制備

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2

7.2

測(cè)定參考條件

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2

7.3

定性測(cè)定

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3

7.4

定量測(cè)定

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3

7.5

空白試驗(yàn)

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4

8

試驗(yàn)數(shù)據(jù)處理

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4

9

回收率

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4

10

精密度

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4

附錄A（資料性）

標(biāo)準(zhǔn)品的信息

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5

附錄B（資料性）

標(biāo)準(zhǔn)溶液多反應(yīng)監(jiān)測(cè)色譜圖

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6

附錄C（資料性）

方法回收率偏差范圍

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7

參考文獻(xiàn)

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8

Ⅰ

GB/T44428—2024

前言

本文件按照GB/T1.1—2020《標(biāo)準(zhǔn)化工作導(dǎo)則第1部分：標(biāo)準(zhǔn)化文件的結(jié)構(gòu)和起草規(guī)則》的規(guī)

定起草。

請(qǐng)注意本文件的某些內(nèi)容可能涉及專(zhuān)利。本文件的發(fā)布機(jī)構(gòu)不承擔(dān)識(shí)別專(zhuān)利的責(zé)任。

本文件由中國(guó)輕工業(yè)聯(lián)合會(huì)提出。

本文件由全國(guó)香料香精化妝品標(biāo)準(zhǔn)化技術(shù)委員會(huì)（SAC/TC257）歸口。

本文件起草單位：上海市質(zhì)量監(jiān)督檢驗(yàn)技術(shù)研究院、上海市食品藥品檢驗(yàn)研究院、上海海關(guān)動(dòng)植物

與食品檢驗(yàn)檢疫技術(shù)中心、上海香料研究所有限公司、清華大學(xué)。

本文件主要起草人：薛峰、楊晉青、印杰、鄭榮、陳丹丹、牛云蔚、陳章庭、楊斌、陳瑤、

喻怡靜、彭興盛、石玲玲、楊振宇、顧宇翔、王怡、張巖、王正。

Ⅲ

GB/T44428—2024

引言

本文件的被測(cè)物質(zhì)是我國(guó)《化妝品安全技術(shù)規(guī)范（2015年版）》規(guī)定的禁用原料。

禁用原料是指不得作為化妝品原料使用的物質(zhì)。《化妝品安全技術(shù)規(guī)范（2015年版）》規(guī)定：若技

術(shù)上無(wú)法避免禁用物質(zhì)作為雜質(zhì)帶入化妝品時(shí)，國(guó)家有限量規(guī)定的應(yīng)符合其規(guī)定；未規(guī)定限量的，應(yīng)進(jìn)

行安全性風(fēng)險(xiǎn)評(píng)估，確保在正常、合理及可預(yù)見(jiàn)的使用條件下不得對(duì)人體健康產(chǎn)生危害。

目前我國(guó)尚未規(guī)定這些物質(zhì)的限量值，本文件的制定，僅對(duì)化妝品中測(cè)定這些物質(zhì)提供檢測(cè)方法。

Ⅳ

GB/T44428—2024

化妝品中大麻二酚和四氫大麻酚的測(cè)定

液相色譜?串聯(lián)質(zhì)譜法

警示─使用本文件的人員應(yīng)有正規(guī)實(shí)驗(yàn)室工作的實(shí)踐經(jīng)驗(yàn)。本文件并未指出所有可能的安全問(wèn)題。

使用者有責(zé)任采取適當(dāng)?shù)陌踩徒】荡胧⒈ＷC符合國(guó)家有關(guān)規(guī)定的條件。

1范圍

本文件描述了化妝品中大麻二酚和四氫大麻酚的液相色譜?串聯(lián)質(zhì)譜測(cè)定方法的原理、試劑和材

料、儀器設(shè)備、試樣制備和試驗(yàn)步驟、試驗(yàn)數(shù)據(jù)處理、回收率和精密度等內(nèi)容。

本文件適用于水劑類(lèi)、膏霜類(lèi)、乳液類(lèi)、凝膠類(lèi)等化妝品中大麻二酚和四氫大麻酚的測(cè)定。

本文件不適用于清潔類(lèi)化妝品和全油基化妝品。

本文件中大麻二酚和四氫大麻酚的方法檢出限均為5μg/kg，定量限均為10μg/kg。

2規(guī)范性引用文件

下列文件中的內(nèi)容通過(guò)文中的規(guī)范性引用而構(gòu)成本文件必不可少的條款。其中，注日期的引用文

件，僅該日期對(duì)應(yīng)的版本適用于本文件；不注日期的引用文件，其最新版本（包括所有的修改單）適用

于本文件。

GB/T6682分析實(shí)驗(yàn)室用水規(guī)格和試驗(yàn)方法

3術(shù)語(yǔ)和定義

本文件沒(méi)有需要界定的術(shù)語(yǔ)和定義。

4原理

試樣經(jīng)甲醇渦旋振蕩，超聲提取，離心過(guò)濾后，得到目標(biāo)物待測(cè)溶液。將目標(biāo)物待測(cè)溶液注入液相

色譜?串聯(lián)質(zhì)譜儀，采用液相色譜分離，質(zhì)譜檢測(cè)器檢測(cè)，保留時(shí)間和質(zhì)譜的相對(duì)離子對(duì)豐度定性，內(nèi)

標(biāo)法定量。

5試劑和材料

除非另有規(guī)定，僅使用色譜純?cè)噭?/p>

5.1水，GB/T6682，一級(jí)。

5.2甲酸銨。

5.3標(biāo)準(zhǔn)品：大麻二酚（CBD）和四氫大麻酚（THC）純度大于99%；四氫大麻酚?D3（THC?D3）

純度大于98%，或經(jīng)國(guó)家