NEB內(nèi)切酶產(chǎn)品具體Buffer活性表_第1頁
NEB內(nèi)切酶產(chǎn)品具體Buffer活性表_第2頁
NEB內(nèi)切酶產(chǎn)品具體Buffer活性表_第3頁
NEB內(nèi)切酶產(chǎn)品具體Buffer活性表_第4頁
NEB內(nèi)切酶產(chǎn)品具體Buffer活性表_第5頁
已閱讀5頁，還剩20頁未讀， 繼續(xù)免費(fèi)閱讀

下載本文檔

版權(quán)說明：本文檔由用戶提供并上傳，收益歸屬內(nèi)容提供方，若內(nèi)容存在侵權(quán)，請(qǐng)進(jìn)行舉報(bào)或認(rèn)領(lǐng)

文檔簡介

/NEBufferActivityChartforRestrictionEnzymesNewEnglandBiolabsprovidesacolor-coded10XNEBufferwitheveryrestrictionendonucleasetoensure100%activity.Tohelpselectthebestconditionsfordoubledigests,thischartshowstheoptimal(supplied)NEBufferandapproximateactivityinthefourstandardNEBuffersforeachenzyme.IfBSAissuppliedwithanenzyme,itisincludedinallNEBufferactivityreactions.Inaddition,thetablealsoshowsrecommendedreactiontemperature,heatinactivationtemperatureandrecommendeddiluentbuffer.Pleaseclickhereforadditionalinformationabout:

UniqueNEBufferActivity

|DoubleDigestion|HeatInactivation|Activityat37°C|DiluentBuffers|Time-SaverEnzymes|HighFidelity(HF)RestrictionEnzymesThischarthasbeenupdatedtoreflectthechangeof54restrictionenzymestoNEBuffer4onNov.1,2008.ClickfortheChartLegend,IconDescriptionA|B|C|D|E|F|H|I|K|M|N|P|R|S|T|X|Z|EnzymeSuppliedNEBuffer%ActivityinNEBuffersHeat

Inactiva-

tion°CIncuba.

Temp.

°CDiluent1234AatIINEBuffer4050501006537AAcc65INEBuffer3+BSA1075100256537AAccINEBuffer45050101008037AAciINEBuffer32550100506537AAclINEBuffer4+BSA10100100No37BAcuINEBuffer4+SAM50100501006537BAfeINEBuffer42550251006537AAflIINEBuffer4+BSA50100251006537AAflIIINEBuffer3+BSA2575100508037BAgeINEBuffer11005010756537CAhdINEBuffer4+BSA257501006537AAleINEBuffer41025101006537AAluINEBuffer4100100751006537BAlwINEBuffer450100101006537AAlwNINEBuffer450100501006537AApaINEBuffer4+BSA255001006525AApaLINEBuffer4+BSA10010010100No37AApeKINEBuffer3257510050No75BApoINEBuffer3+BSA1075100758050AAscINEBuffer4010101006537AAseINEBuffer3NR75100ddNR6537BAsiSINEBuffer4+BSA501001001008037BAvaINEBuffer41075101008037AAvaIINEBuffer45075101006537AAvrIINEBuffer4100100501008037BEnzyme

topSuppliedNEBuffer%inB1%inB2%inB3%inB4Heat

Inactiva.Incuba.

Temp.DiluentBaeGINEBuffer11007510506537ABaeINEBuffer4+BSA+SAM50100501006525ABamHINEBuffer3+BSA75100100dd100No37ABamHI-HF?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

topSuppliedNEBuffer%inB1%inB2%inB3%inB4Heat

Inactiva.Incuba.

Temp.DiluentCac8INEBuffer450751001006537BClaINEBuffer4+BSA1050501006537ACspCINEBuffer4+SAM10100101006537ACviAIINEBuffer47525101006525BCviKI-1NEBuffer41050251008037ACviQINEBuffer3+BSA7510010075No25BEnzyme

topSuppliedNEBuffer%inB1%inB2%inB3%inB4Heat

Inactiva.Incuba.

Temp.DiluentDdeINEBuffer375100100756537ADpnINEBuffer4100100751008037BDpnIINEBufferDpnIINRNR100ddNR6537BDraINEBuffer47575501006537ADraIIINEBuffer3+BSA10075100256537BDrdINEBuffer4+BSA2550101006537AEnzyme

topSuppliedNEBuffer%inB1%inB2%inB3%inB4Heat

Inactiva.Incuba.

Temp.DiluentEaeINEBuffer4100100501008037AEagINEBuffer31025100106537CEagI-HF?NEBuffer42550101006537CEarINEBuffer4100100501006537AEciINEBuffer2+BSA50100100506537AEcoNINEBuffer4100100751006537AEcoO109INEBuffer4+BSA100100751006537AEcoP15INEBuffer3+BSA+ATP751001001006537AEcoRINEBufferEcoRIdd1001001001006537CEcoRI-HF?NEBuffer41010001006537CEcoRVNEBuffer3+BSA5075100508037AEcoRV-HF?NEBuffer4251001001006537BEnzyme

topSuppliedNEBuffer%inB1%inB2%inB3%inB4Heat

Inactiva.Incuba.

Temp.DiluentFatINEBuffer21010050506555AFauINEBuffer410050101006555AFnu4HINEBuffer41025251006537AFokINEBuffer4100100751006537AFseINEBuffer4+BSA1007501006537BFspINEBuffer4107510100dd6537CEnzyme

topSuppliedNEBuffer%inB1%inB2%inB3%inB4Heat

Inactiva.Incuba.

Temp.DiluentHaeIINEBuffer4+BSA75100501008037AHaeIIINEBuffer450100251008037AHgaINEBuffer110075501006537AHhaINEBuffer4+BSA751001001006537AHincIINEBuffer3+BSA751001001006537BHindIIINEBuffer25010010506537BHinfINEBuffer475100751008037AHinP1INEBuffer41001001001006537AHpaINEBuffer4255010100No37AHpaIINEBuffer11005010506537AHphINEBuffer4NR7501006537BHpy166IINEBuffer4100100501006537CHpy188INEBuffer45025101006537AHpy188IIINEBuffer4+BSA100100101006537AHpy99INEBuffer4+BSA101001006537AHpyAVNEBuffer4+BSA100100251006537BHpyCH4IIINEBuffer410050251008037AHpyCH4IVNEBuffer11002510256537AHpyCH4VNEBuffer45050251006537BEnzyme

topSuppliedNEBuffer%inB1%inB2%inB3%inB4Heat

Inactiva.Incuba.

Temp.DiluentI-CeuINEBuffer4+BSA101001006537BI-SceINEBufferI-SceI+BSA105050506537BEnzyme

topSuppliedNEBuffer%inB1%inB2%inB3%inB4Heat

Inactiva.Incuba.

Temp.DiluentKasINEBuffer4+BSA2510001006537BKpnINEBuffer1+BSA10075050No37AEnzyme

topSuppliedNEBuffer%inB1%inB2%inB3%inB4Heat

Inactiva.Incuba.

Temp.DiluentMboINEBuffer4751001001006537AMboIINEBuffer4100100501006537CMfeINEBuffer47550101006537AMfeI-HF?NEBuffer47550101006537AMluINEBuffer32575100506537AMlyINEBuffer4+BSA5050251006537AMmeINEBuffer4+SAMNRNRNR100dd8037BMnlINEBuffer4+BSA75100501006537BMscINEBuffer47575751006537BMseINEBuffer4+BSA75100751006537AMslINEBuffer4100100251006537AMspA1INEBuffer4+BSA50100501006537BMspINEBuffer475100501008037AMwoINEBuffer3107510075No60BEnzyme

topSuppliedNEBuffer%inB1%inB2%inB3%inB4Heat

Inactiva.Incuba.

Temp.DiluentNaeINEBuffer410075101006537ANarINEBuffer410075751006537ANb.BbvCINEBuffer250100101008037ANb.BsmINEBuffer325100100258065ANb.BsrDINEBuffer21010025508065ANb.BtsINEBuffer4+BSA75100751008037ANciINEBuffer41002510100No37ANcoINEBuffer31001001001006537ANcoI-HF?NEBuffer450100101008037BNdeINEBuffer475100751006537ANgoMIVNEBuffer410050101008037ANheINEBuffer2+BSA100100101006537CNheI-HF?NEBuffer4+BSA1001001008037CNlaIIINEBuffer4+BSA2525251006537BNlaIVNEBuffer4+BSA1010101006537BNmeAIIINEBuffer4+SAM101001008037BNotINEBuffer3+BSA050100256537CNotI-HF?NEBuffer4+BSA25100251006537ANruINEBuffer3010100106537ANsiINEBuffer31075100258037ANspINEBuffer2+BSA10010001006537ANt.AlwINEBuffer22510025508037ANt.BbvCINEBuffer450100101008037ANt.BsmAINEBuffer410050101006537ANt.BspQINEBuffer31075100258050BNt.BstNBINEBuffer3010100108055ANt.CviPIINEBuffer425100501006537AEnzyme

topSuppliedNEBuffer%inB1%inB2%inB3%inB4Heat

Inactiva.Incuba.

Temp.DiluentPacINEBuffer1+BSA10075101006537APaeR7INEBuffer42510010100No37APciINEBuffer35075100508037APflFINEBuffer4+BSA025251006537APflMINEBuffer3+BSA0100100506537APhoINEBuffer3505010075No75API-PspINEBufferPI-PspI+BSA0101010No65BPI-SceINEBufferPI-SceI+BSA00006537BPleINEBuffer4100100751006537APmeINEBuffer4+BSA050101006537APmlINEBuffer1+BSA100750756537APpuMINEBuffer40250100No37BPshAINEBuffer4+BSA5075101006537APsiINEBuffer410100101006537BPspGINEBuffer47510050100No75APspOMINEBuffer42525101006537APspXINEBuffer40100101008037BPstINEBuffer3+BSA7575100508037CPvuINEBuffer3+BSA1075100108037BPvuIINEBuffer2100100100100No37BPvuII-HF?NEBuffer402501008037BEnzyme

topSuppliedNEBuffer%inB1%inB2%inB3%inB4Heat

Inactiva.Incuba.

Temp.DiluentRsaINEBuffer4100100501006537ARsrIINEBuffer42575101006537CEnzyme

topSuppliedNEBuffer%inB1%inB2%inB3%inB4Heat

Inactiva.Incuba.

Temp.DiluentSacINEBuffer1+BSA10050101006537ASacI-HF?NEBuffer4+BSA10050101006537ASacIINEBuffer42575101006537ASalINEBuffer3+BSA0010006537ASalI-HF?NEBuffer4101001001006537ASapINEBuffer4755001006537CSau3AINEBuffer1+BSA10050101006537ASau96INEBuffer4501001001008037ASbfINEBuffer4755001006537ASbfI-HF?NEBuffer4502501006537BScaINEBuffer3NRNR100ddNR8037AScaI-HF?NEBuffer4100100101006537BScrFINEBuffer41001001001006537ASexAINEBuffer4+BSA10075501006537ASfaNINEBuffer3075100256537BSfcINEBuffer4+BSA7550101006537BSfiINEBuffer4+BSA010010100No50CSfoINEBuffer42510050100No37BSgrAINEBuffer410050101006537ASmaINEBuffer40001006525ASmlINEBuffer4+BSA257525100No55ASnaBINEBuffer4+BSA255025100dd8037ASpeINEBuffer4+BSA75100251008037CSphINEBuffer2100100501006537BSphI-HF?NEBuffer45025101

溫馨提示

  • 1. 本站所有資源如無特殊說明，都需要本地電腦安裝OFFICE2007和PDF閱讀器。圖紙軟件為CAD,CAXA,PROE,UG,SolidWorks等.壓縮文件請(qǐng)下載最新的WinRAR軟件解壓。
  • 2. 本站的文檔不包含任何第三方提供的附件圖紙等，如果需要附件，請(qǐng)聯(lián)系上傳者。文件的所有權(quán)益歸上傳用戶所有。
  • 3. 本站RAR壓縮包中若帶圖紙，網(wǎng)頁內(nèi)容里面會(huì)有圖紙預(yù)覽，若沒有圖紙預(yù)覽就沒有圖紙。
  • 4. 未經(jīng)權(quán)益所有人同意不得將文件中的內(nèi)容挪作商業(yè)或盈利用途。
  • 5. 人人文庫網(wǎng)僅提供信息存儲(chǔ)空間，僅對(duì)用戶上傳內(nèi)容的表現(xiàn)方式做保護(hù)處理，對(duì)用戶上傳分享的文檔內(nèi)容本身不做任何修改或編輯，并不能對(duì)任何下載內(nèi)容負(fù)責(zé)。
  • 6. 下載文件中如有侵權(quán)或不適當(dāng)內(nèi)容，請(qǐng)與我們聯(lián)系，我們立即糾正。
  • 7. 本站不保證下載資源的準(zhǔn)確性、安全性和完整性, 同時(shí)也不承擔(dān)用戶因使用這些下載資源對(duì)自己和他人造成任何形式的傷害或損失。

評(píng)論

0/150

提交評(píng)論