免疫濁度測(cè)定及其臨床應(yīng)用課件

免疫濁度測(cè)定及其臨床應(yīng)用1免疫測(cè)定分類免疫濁度測(cè)定

g~

ng標(biāo)記免疫測(cè)定ng~

pg免疫濁度測(cè)定免疫沉淀反應(yīng)海德堡曲線Heidelberg’s

Curve抗原濃度測(cè)定范圍抗體過剩抗原過剩Ag

*Ab免疫濁度測(cè)定分類透射濁度法散射濁度法透射濁度法和散射濁度法的光路檢測(cè)器A(透射濁度法)檢測(cè)器B(散射濁度法)θI0I渾濁樣品光源散射濁度法AnalyteAntibodyOther

stuffAnalyte-antibody

complexingNow

big

enough

to

scatter

lightToo

small

to

detect

opticallyWhat

happens

inside

the

flowcell?31ScatterScatter

Signal

Versus

TimeNephelometry1.

Buffer

additionTime2.

Sample

Addition3.

Antibody

additionFluid

in

the

cell

has

causes

little

scatter2Adding

sample

increases

scatter

slightlyAdding

antibody

causes

a

largeincrease

in

light

scattering免疫濁度測(cè)定分類2終點(diǎn)法速率法終

點(diǎn)

法固定時(shí)間法速率散射濁度法速率峰的測(cè)定速率峰的測(cè)定ScatterMeasure

the

RATE

After

Antibody

AdditionRate

Nephelometry321Time1.

Buffer

addition 2.

Sample

Addition 3.

Antibody

additionFirst

derivative:

rate

of

change

of

scatter

over

time;this

is

the

SLOPE

of

thecurveRatePEAKRATETIMERATERate

of

Change

of

Scatter

Signal

versus

TimeCONCENTRATIONRATE在速率峰基礎(chǔ)上完成的標(biāo)準(zhǔn)曲線抗原過剩的測(cè)定Analyte-antibody

complexingAnalyteAntibodyAntigen

ExcessRATEofSCATTER抗體過量時(shí)的反應(yīng)抗原過量時(shí)的反應(yīng)60085反應(yīng)時(shí)間(秒)ARRAY抗原過量自動(dòng)檢測(cè)加入校正液藥物濃度測(cè)定免疫濁度抑制測(cè)定(小分子物質(zhì))12Conjugate-antibody

complexing213Inhibition

of

complexing

by

hapten

(drug)Conjugate

(hapten

on

carrier)AntibodyHapten

(drug)免疫濁度測(cè)定試劑抗體試劑的要求1特異性2親和力3反應(yīng)時(shí)保持抗體過剩455測(cè)定范圍應(yīng)包括高于及低于正常值的50%測(cè)定的精密度應(yīng)在5%左右校正曲線：聚乙二醇的增強(qiáng)作用抗原濃度C1C2C3吸光度4%聚乙二醇不加聚乙二醇試劑組成配方舉例~

8.2試劑1100mmol Tris或磷酸緩沖液

pH7.24% 聚乙二醇(分子量6000或10000)1%非離子型表面活性劑150mmol氯化鈉或硫酸鈉0.1

g/l

疊氮鈉~

8.2試劑2100mmol Tris或磷酸緩沖液

pH7.220% 多克隆羊抗血清150mmol氯化鈉0.1

g/l

疊氮鈉抗原濃度測(cè)定范圍抗體過?？乖^剩Ag

*Ab技術(shù)特性并行的檢測(cè)系統(tǒng):Rate

NIPIARate

NephelometryNIPIA：近紅外顆粒速率法免疫分析BenefitsAbility

to

expand

the

menuinto

high

sensitivity

assaysincluding:

Ferritin,

Digoxin,

and

IgE.NIR

eliminates

the

interferenceof

biological

substancessuch

as

biliribin

andhemoglobin.NIPIA：近紅外顆粒速率法免疫分析優(yōu)點(diǎn)擴(kuò)展檢測(cè)菜單，增加高敏項(xiàng)目分析：

如鐵蛋白，地高辛 ，IgE，超敏CRP等近紅外波長(zhǎng)分析消除生物物質(zhì)如膽紅素和血色素的干擾NIR：940nm波長(zhǎng)近0oC檢測(cè)角檢測(cè)顆粒大小：200~500nm直徑速率法測(cè)定NIPIA：940nmLED光源速率散射法：670nm激光光源蛋白應(yīng)用分類心血管功能監(jiān)測(cè)載脂蛋白A1載脂蛋白B脂蛋白a11、最靈繁的人也看不見自己的背脊。、最靈繁的人也看不見自己

的背脊。————非洲非洲22、最困難的事情就是認(rèn)識(shí)自己。、最困難的事情就是認(rèn)識(shí)自己?！ＥD希臘33、有勇氣承擔(dān)命運(yùn)這才是英雄好漢。、有勇氣承擔(dān)命運(yùn)這才是英

雄好漢?！谌谌?4、與肝膽人共事，無(wú)字句處讀書。、與肝膽人共事，無(wú)字句處讀