文稿致病性球菌

PathogenicOtherLaiXiaomin(賴小敏）DepartmentofMicrobiologyHighlightsofConceptofpathogeniccocci Staphylococcus(Esp.,S.aureus)56minStreptococcus(Esp.,S.pyogenes)38minEnterococcusspeciesandVRE NeisseriameningitidesNeisseria OtherPseudomonas Withregardtoeachpathogen,thefollowingswillbetalked:MicrobiologicalPreventionandPustulebySPustuleS.S.SorethroatbySUntreatederysipelas(丹毒)bySwithskinsloughingandbyflesh-eatingbacteria(invasivestreptococcus,anaerobicbacteria,etc.),andinsects(bee,etc.)Chestx-rayFilmjustafewhoursofrigorsandproductivecough

Chestx-rayFilm12hoursconsolidationintherightupperlobeLobarpneumoniabyS.byN.meningitidis,widespreadGonococcalconjunctivitisbyN.CausativeagentsofaboveclinicalPathogenicWhatarePathogeniccocci?alsocalled—Including---Staphylococcus(S.aureus&S.(A群鏈球菌orImportantGram Bacterial GenusGenus Genus(N.meningitidesN.ImportantpyogenicLeadingpathogenofwoundinfections(includingotherstaphylococci)BiologicalShape,Stain&spherical,elliptical,irregulargrape-likeclusterGram+(G+)GrowwellonbasicAntigenicstructureStaphylococcalproteinA(SPA)OnekindofsurfaceproteinincellwallSinglechainpeptideActions—Binds(bacterium)tohost’sIgG1,IgG2&coagglutinationCompetesw/FcreceptorsofCoagglutinationtestwithinert(e.g.,heat-killedS.aureusorlatexSPAcaninhibitphagocytosisbybindingwithFcreceptorsofTeichoicacidpolysaccharideA—S.polysaccharideB—S.PhasesofImportantPropertiesof3MainSpeciesS.S.S.PigmentsonGolden-Whiteor+——++—+--a-a-+——Heat-stable+——+——TeichoicAdonit-Glycerine- Pathogenicity&

Cytotoxins(Staphylolysina,b,,&d,Leukocidin),Exfoliatin,Enterotoxins,&TSST-1FreeFreeCoagulaseactivated (游離凝固酶) PlasmatobeTubecoagulaseBoundcoagulase(結(jié)合凝固酶)orplasmatobeclotedorbacteriatoProtectionactionsStaphylolysinclassifiedintoa-,b-,-,&d-a-toxin—makesporesincellkillsordegranulatesneutrophilsExfoliatinincludingtypeA&typecausesstaphylococcalscaldedskinEnterotoxinincludingserotypesA,B,C1-3,D,E,G,&mainlycausealsoactsassuperantigenToxicshocksyndrometoxin1(TSST-(毒性休克綜合征毒素-1causestoxicshocksyndromeonekindofHeat-stablenucleaseClinicalInvasive-local-systemicToxin-mediated-food-staphylococcalscaldedskinsyndrome-toxicshocksyndromeFolliculitisbySBullousImpetigobyS.AS.aureusinfectionona12-year-oldchild'sPathogenesisofstaphylococcal Pus,blood,food,orSmear

G+Colonypigments& t CoagulasetestMannitefermentationHeat-stablenucleaseAnimalIdentificationofpathogenicCoagulaseHeat-stablenuclease(+)Goldenpigment(+)Hemolyzation(+)Mannitefermentation(+) sensitivity

&

Methicillin-resistantS.aureus,Comparethefeaturesofcommonpyogenicbacteria.DiscussthepathogenicfeaturesandidentificationpointsofStaphylococcusaureus.BiologicalG+,spherical,elliptical,arrangedinGrowwellonnutrientmediumw/noAntigenicProtein—surfaceAg,outsideC—includingM,T,R,SinGroup—C

Cell—P CellCell

According

groupA-V,total20groupsamongpathogenicstreptococci90%isgroupover100typesaccordingtoMgroupB,C,D,&GGroupA

ProteinG:similartoProtein-lipoteichoicacid-fibronectinbindingprotein(FBP,proteinF)-protein

(SPE,鏈球菌致熱外毒素)Streptokinase(SK,鏈激酶)-proteinw/SH-destroysmanykindsof-anti-SLOtest(ASOtest)helpfulfordiagnosis-lowMW-causesb-hemolysisonBloodAgarStreptococcalpyrogenicexotoxin-codedbylysogenic-proteinsincludingserotypesA,B,-causesscarletfever-onekindof-alsocausesHyaluronidase-alsocalledasspreadingfactor-hydrolyzeshyaluronicacidinconnective-helpfulforspreadingof-alsocalledasstreptococcal-hydrolyzesClinicaldiseases-scarletfever-streptococcaltoxin-mediated(late-rheumaticfever-acuteglomeruloniphritisErysipelasbySCellulitisbyS"Honey-crust"lesiontypicalofS.pyogenesPathogenesisofS.pyogenes Pus,Blood,NasopharynxSmearIsolationw/bloodagarplate

G+Othera- ASO Assist-diagnosisOtherS.pneumoniaeGroupBstreptococciGroupDViridans(a-hemolytic)StreptococcussuisEnterococcusspecies&EnterococcusSpeciesandPreviouslyclassifiedGroupDIn1984,namedasgenusEnterococcusGram+cocciinpairsorshortchainsMostarefacultativeanaerobesComplexnutritionalrequirementsGroupDspecificteichoicacidantigenEnterococcusspp.viewedbydirectfluorescentantibody(FA)Commonlyfoundintheintestinesofnormalhealthyindividuals(humansandanimals).2importanthumandiseases-relatedEfaecalisandEfaeciumUrinarytractinfectionsBacteremia&septicaemiaSubacuteendocarditisWoundinfectionsFoodbornediseasesOneofimportantlife-nosocomialpathogensSince1986,VREinfectionshavebecomeagrowingproblemDiscussthepathogenicfeaturesDiscussidentificationpointsbetweenhemolyticstreptococciandotherpyogenicbacteria.NeisseriaCausesepidemiccerebrospinal(流行性腦脊髓膜炎 流腦byN.meningitidis,widespreadNeisseriaBiologicalShapeandStain-G-diplococciinoronPMNsonpus-Noendospore&flagellum,&w/capsule&Culture-Obligateaerobes,need5%CO2whileprimaryisolation-HighnutritionalAntigenicstructureandclassification-Pilusprotein-Outermembraneproteinantigens：PI,PII,&-LOS(lipooligosaccharide脂寡糖LPS-N.gonorrhoeaeVirulent--Outermembrane--IgA1-Clinical-in

Gonorrheain

-PelvicClinicalmanifestationsofN.gonorrhoeaeHumoralimmunitymoreeffectiveNopersistentimmuneresponsesInfectiontrendsto-chronic&repeated Pussecretafromgenito-urinarytracts,eyes,&Smear

G-inoron Coloniesµscopic&serologictests

OxidaseSugarfermentationtestOther Nucleicacidsdetection,&soPrevention&TakepreventionsasmainmeasureChoosepenicillinasNo.1drug,takecareofproble