編制說明-腹瀉糞便標(biāo)本中溶藻弧菌檢驗(yàn)

《腹瀉糞便標(biāo)本中溶藻弧菌檢驗(yàn)》編制說明1依據(jù)《北京預(yù)防醫(yī)學(xué)會(huì)團(tuán)體標(biāo)準(zhǔn)管理辦法(2023年版)》，按照《關(guān)于開展疾病預(yù)防控制中心向北京預(yù)防醫(yī)學(xué)會(huì)標(biāo)準(zhǔn)化管理辦公室提出申請(qǐng)，2023年3月2版本、征求意見稿、送審稿、報(bào)批稿的核定，本、征求意見稿、送審稿、報(bào)批稿的核定，王建國(guó)：方法技術(shù)指標(biāo)的確定及送審稿、報(bào)3敗血癥、中耳炎等疾病。超過20種弧菌可引法國(guó)比斯開灣一份關(guān)于2001-2019年弧菌感染的臨床流行病學(xué)調(diào)查報(bào)告顯示溶種病理有關(guān)，病死率為7.14%。我國(guó)海近些年在腹瀉疫情的處置中會(huì)發(fā)現(xiàn)一些臨床表現(xiàn)與副溶血性弧菌引起的疾（1）國(guó)外的標(biāo)準(zhǔn)都是針對(duì)弧菌屬，尤其對(duì)副溶血性弧菌、霍亂弧菌、創(chuàng)傷②弧菌檢驗(yàn)的國(guó)家標(biāo)準(zhǔn)有副溶血性弧菌和創(chuàng)傷弧菌③弧菌屬的檢驗(yàn)方法多是十幾年前發(fā)布的，沒有引入分子生物學(xué)、④僅有的溶藻弧菌檢驗(yàn)標(biāo)準(zhǔn)是出入境機(jī)構(gòu)關(guān)于出口食品中溶藻弧菌的檢驗(yàn)4[1]王海波,張京云,王多春等.溶藻弧菌TaqMan實(shí)時(shí)PCR快速檢測(cè)體系的建立[J].中國(guó)衛(wèi)生檢驗(yàn)雜志,2011,21(05)[2]GB4789.7-2013食品安全國(guó)家標(biāo)準(zhǔn)食品微生物學(xué)檢驗(yàn)副溶血性弧菌主要技術(shù)內(nèi)容在增菌培養(yǎng)基、選擇性分離培養(yǎng)基、鑒定方篩的條件摸索方面，綜合考慮溶藻弧菌及各種腸道致病菌在培養(yǎng)基中的生長(zhǎng)表現(xiàn)，最后選定3%氯化鈉堿性蛋白胨水為溶藻弧菌5中培養(yǎng)過夜，轉(zhuǎn)種于3%氯化鈉胰蛋白胨大豆瓊脂，過夜培養(yǎng)。分別用VITEK系生化結(jié)果：30個(gè)菌株VITEK細(xì)菌生化鑒定系統(tǒng)給出的拉丁文鑒定結(jié)果均為萄球菌CICC23656、金葡201716MALDI-TOFMS驗(yàn)證結(jié)果：志賀CFU)。用溶藻弧菌質(zhì)控球調(diào)制高濃度菌液1CFU/mL～10CFU/mL,低濃度菌液5份；將低濃度菌液1mL加入一份加了干擾菌的糞便標(biāo)本，制作40份；其他5別標(biāo)本號(hào)增菌液熒光PCR初篩結(jié)果鑒定結(jié)果Ct值生化結(jié)果（VITEK報(bào)告拉丁文名稱）生化結(jié)果可信度（%）質(zhì)譜結(jié)果(拉丁文名稱)質(zhì)譜評(píng)分熒光PCR結(jié)果性/陽(yáng)性)熒光PCR（Ct值）高濃度1237453低濃度67N/A//////89N/A//////N/A//////N/A//////N/A////////////8N/A//////N/A//////N/A//////對(duì)照組N/A//////N/A//////N/A//////N/A//////N/A//////9algalgiolytalgiolytic濃度類別標(biāo)本號(hào)增菌液熒光PCR初篩結(jié)果鑒定結(jié)果陰性/Ct值生化結(jié)果（VITEK報(bào)告拉丁文名稱）生化結(jié)果可信質(zhì)譜結(jié)果(拉丁文名稱)質(zhì)譜評(píng)分（布魯熒光PCR結(jié)/陽(yáng)性)熒光值）高濃度114.43Vibrioalginolyt99%Vibrioalginolytic811.21214.14Vibrioalginolyt99%Vibrioalginolytic211.35314.08Vibrioalginolyt98%Vibrioalginolytic311.20414.31Vibrioalginolyt99%Vibrioalginolytic311.47514.24Vibrioalginolyt99%Vibrioalginolytic2.07012.41低濃度614.13Vibrioalginolyt99%Vibrioalginolytic412.41714.16Vibrioalginolyt97%Vibrioalginolytic411.10814.60Vibrioalginolyt98%Vibrioalginolytic013.10914.68Vibrioalginolyt96%Vibrioalginolytic511.7614.08Vibrioalginolyt99%Vibrioalginolytic312.2914.38Vibrioalginolyt99%Vibrioalginolytic911.8814.51Vibrioalginolyt90%Vibrioalginolytic511.0215.05Vibrioalginolyt99%Vibrioalginolytic811.8114.17Vibrioalginolyt99%Vibrioalginolytic511.3314.78Vibrioalginolyt98%Vibrioalginolytic812.2315.13Vibrioalginolyt99%Vibrioalginolytic011.6415.27Vibrioalginolyt99%Vibrioalginolytic411.4514.79Vibrion97%Vibrion812.2014.99Vibrioalginolyt93%Vibrioalginolytic112.3914.97Vibrioalginolyt98%Vibrioalginolytic013.4415.13Vibrioalginolyt99%Vibrioalginolytic812.71N/A//////15.20Vibrioalginolyt99%Vibrioalginolytic411.9915.02Vibrioalginolyt99%Vibrioalginolytic610.7015.22Vibrioalginolyt99%Vibrioalginolytic2.00813.3415.26Vibrioalginolyt99%Vibrioalginolytic411.2514.33Vibrioalginolyt99%Vibrioalginolytic311.3214.59Vibrioalginolyt99%Vibrioalginolytic012.3114.79Vibrioalginolyt98%Vibrioalginolytic2.17611.8213.44Vibrioalginolyt98%Vibrioalginolytic210.6214.21Vibrioalginolyt98%Vibrioalginolytic312.0714.48Vibrioalginolyt99%Vibrioalginolytic711.9714.65Vibrioalginolyt99%Vibrioalginolytic811.3014.06Vibrioalginolyt96%Vibrioalginolytic611.8514.36Vibrioalginolyt99%Vibrioalginolytic511.7814.23Vibrioalginolyt99%Vibrioalginolytic813.0714.48Vibrioalginolyt99%Vibrioalginolytic512.2513.58Vibrioalginolyt99%Vibrioalginolytic2.03412.0814.43Vibrioalginolyt99%Vibrioalginolytic011.684014.73Vibrioalginolyt99%Vibrioalginolytic2.01511.5641N/AVibrioalginolyt99%Vibrioalginolytic112.124214.79Vibrioalginolyt98%Vibrioalginolytic712.534314.43Vibrioalginolyt99%Vibrioalginolytic611.934414.31Vibrioalginolyt99%Vibrioalginolytic411.534514.74Vibrioalginolyt99%Vibrioalginolytic812.96對(duì)照組46N/A//////47N/A//////48N/A//////49N/A//////N/A//////濃度類別標(biāo)本號(hào)增菌液熒光PCR初篩結(jié)果鑒定結(jié)果/陽(yáng)性Ct值生化結(jié)果（VITEK報(bào)告拉丁文名稱）生化結(jié)果可信度質(zhì)譜結(jié)果稱)質(zhì)譜評(píng)分魯熒光PCR結(jié)果（陰性/陽(yáng)性)熒光值）高濃度114.33Vibrioalginolyti99%Vibrioalginolyt2.29912.51214.12Vibrioalginolyti99%Vibrioalginolyt2.24611.65314.07Vibrioalginolyti96%Vibrioalginolyt2.30811.45414.56Vibrioalginolyti95%Vibrioalginolyt2.26712.41514.14Vibrioalginolyti90%Vibrioalginolyt2.46611.23614.65Vibrioalginolyti96%Vibrioalginolyt2.21511.46714.84Vibrioalginolyti90%Vibrioalginolyt2.21112.40814.68Vibrioalginolyti99%Vibrioalginolyt2.37011.209N/A//////14.65Vibrioalginolyti99%Vibrioalginolyt2.44511.39N/A//////15.53Vibrioalginolyti99%Vibrioalginolyt2.37312.1215.99Vibrioalginolyti99%Vibrioalginolyt2.36012.11N/A//////15.18Vibrioalginolyti99%Vibrioalginolyt2.32911.6515.45Vibrioalginolyti98%Vibrioalginolyt2.30711.4415.17Vibrioalginolyti96%Vibrioalginolyt2.28611.21N/A//////15.33Vibrioalginolyti99%Vibrioalginolyt2.31211.9315.26Vibrioalginolyti99%Vibrioalginolyt2.40712.1515.43Vibrioalginolyti96%Vibrioalginolyt2.19711.73N/A//////15.23Vibrioalginolyti96%Vibrioalginolyt2.37212.14N/A//////15.29Vibrioalginolyti96%Vibrioalginolyt2.37711.7515.66Vibrioalginolyti96%Vibrioalginolyt2.32511.47N/A//////N/A//////14.77Vibrioalginolyti96%Vibrioalginolyt2.33311.3315.42Vibrioalginolyti99%Vibrioalginolyt2.31111.2615.61Vibrioalginolyti95%Vibrioalginolyt2.25510.9915.12Vibrioalginolyti99%Vibrioalginolyt2.41510.9815.98Vibrioalginolyti96%Vibrioalginolyt2.36712.0015.14Vibrioalginolyti96%Vibrioalginolyt2.23011.7414.99Vibrioalginolyti97%Vibrioalginolyt2.35512.01N/A//////15.18Vibrioalginolyti96%Vibrioalginolyt2.28011.8515.28Vibrioalginolyti99%Vibrioalginolyt2.26711.9715.23Vibrioalginolyti99%Vibrioalginolyt2.27012.184015.13Vibrioalginolyti97%Vibrioalginolyt2.21210.9641N/A//////4215.19Vibrioalginolyti99%Vibrioalginolyt2.29911.7743N/A//////4414.31Vibrioalginolyti97%Vibrioalginolyt2.25612.034515.24Vibrioalginolyti97%Vibrioalginolyt2.35511.47對(duì)照組46N/A//////47N/A//////48N/A//////49N/A//////N/A//////濃度類別樣本號(hào)增菌液熒光PCR初篩結(jié)果鑒定結(jié)果陰性性值）生化結(jié)果（VITEK報(bào)告拉丁文名稱）生化結(jié)果可信度)質(zhì)譜結(jié)果(拉丁文名稱)質(zhì)譜評(píng)分 安熒光PCR結(jié)果（陰性/陽(yáng)性)熒光PCR（Ct值）高濃度1Vibrio93Vibrio9.02Vibrioalginolyticus94Vibrioalginolyticus9.2793Vibrioalginolyticus92Vibrioalginolyticus9.3034Vibrioalginolyticus93Vibrioalginolyticus9.3425Vibrioalginolyticus93Vibrioalginolyticus9.476低濃度626.3Vibrioalginolyticus93Vibrioalginolyticus9.3357///////8///////9Vibrio93Vibrio9.225.4Vibrioalginolyticus95Vibrioalginolyticus9.00829.1Vibrioalginolyticus93Vibrioalginolyticus9.268Vibrioalginolyticus93Vibrioalginolyticus9.426Vibrioalginolyticus93Vibrioalginolyticus9.34928.1Vibrioalginolyticus94Vibrioalginolyticus6228.9Vibrioalginolyticus93Vibrioalginolyticus78///////25.4Vibrioalginolyticus95VibrioalginolyticusVibrio93Vibrio9.226.4Vibrioalginolyticus93Vibrioalginolyticus9.0532028.3Vibrioalginolyticus93Vibrioalginolyticus752129.7Vibrioalginolyticus93Vibrioalginolyticus3022Vibrioalginolyticus93Vibrioalginolyticus9923Vibrioalginolyticus93Vibrioalginolyticus9.00224///////2526.4Vibrioalginolyticus93Vibrioalginolyticus2226///////2723.8Vibrioalginolyticus94Vibrioalginolyticus9.4762824.8Vibrioalginolyticus93Vibrioalginolyticus9.42529Vibrio93Vibrio9.53026.6Vibrioalginolyticus95Vibrioalginolyticus9.5313125.5Vibrioalginolyticus93Vibrioalginolyticus9.4423230.3Vibrioalginolyticus93Vibrioalginolyticus9.0023325.3Vibrioalginolyticus93Vibrioalginolyticus9.0343428.4Vibrioalginolyticus93Vibrioalginolyticus9