智能技術(shù)在合成生物學(xué)中的應(yīng)用白皮書

湡?痦?妃欰暟?助?ㄐ展?欰暟?皍? 3??ㄤ???? 3ざ?欰暟??? 3?梠 4???助 52 佟瘻?餴劥鳥ざ?欰暟?????1.2 佟瘻?餴劥鳥ざ?欰暟?????1.2.1篩瘻畮 94 ???瀖GinkgoBio 3 ざ?欰暟?????區(qū)4 ???瀖GinkgoBio 3 ざ?欰暟?????區(qū)1. works?鳴?垷蘭臘??鳥展?欰暟?灇瑕 18ざ?欰暟?涸蘭臘?-?鳥猰??梠 18蘭臘??ざ?欰暟??涸?欽-劼??鸑?荈?????絏 19?跗涯餘?ぢ鵳?鵂玐爙?劼????荈????絕ざ涸?挿 203 嵳?ざ?欰暟?蘭臘??欽??梡朐 .2s?. 3 嵳?ざ?欰暟?蘭臘??欽??梡朐 2.3.1?欽欰暟?鳴?Biofoundry?荈?ざ??鯪?暟294 ??ざ?欰暟?蘭臘??欽??梡朐 .2?a 4 ??ざ?欰暟?蘭臘??欽??梡朐 ぐ垷?霃?????臘 34荈??倰呩涸?? 35?涸?ぢ鵳??癃鷥 37iBioFoundry?霃務(wù)?挿絑? 38勱勻展?欰暟?涸蘭臘??劅 403.1 劼???倰? 40SDLs荈??-荈????倰? 41?梡SDLs涸???稇 43?罌俒柄 44痦?妃欰暟?助?ㄐ展?欰暟?皍???ㄤ?????yteticBiology僽???岤?欰ㄐ禹絡(luò)ㄤ欰暟??宨翸?輑ざ?欰ㄐ猰???玐??暟椚???ㄤ??猰?瘝霽??猰僽梡去欰暟?剒?封?悶?涸?????ざ?欰暟?涸呍???霃雦?佖鳴?????鳴欰暟???欰暟導(dǎo)?禹絡(luò)?去靧鸀??鵂玐勃蕘?鳴?剣欰ㄐ崞?臘?涸絈脅ㄤ欰暟???鵯貓?ざ?欰暟???鵳遤暟餘???ざ?涸欰?倰?剣劅??」?勱勻?蚋???????臘彂?勞俱???瘝?絡(luò)遤?鄄钚?絩DNA?轠偒絕匬封梡1953ㄤ?碫??絆崵?2003??た涸痦?妃欰暟?助?ㄐ?呏研繠????欰灇瑕?NationalInstitutesofHealth,NIH??絆灇瑕?NationalGenomeResearchInstitute???涸??ざ欰暟?僽??覆?欰暟佖鳴涸猰???餻剣倝臘??ざ?暵?暟餘崵梠?瘝???勻霹ざ?欰暟?絕ざ???欰暟??禹絡(luò)欰暟??鷳鵂欰暟?玐涸?椚?倝?欰暟禹絡(luò)霃雦倝?臘雮灇瑕?プ臘???灇瑕?佖」???欨蕘?鳴?撐練涸欰暟騟??DNA??欰暟禹絡(luò)鵳罜?????鳴????瘝倰???剣佪涸鍒?倰呩?ざ?欰暟?涸?欰ㄤ?鳥封?傁僽?碫?欰ㄐ梡韌帿?確程ㄤ禹絡(luò)雩濼?たざ?鶴鰳涸?搬絕卓?僽20?紬劣ㄤ21?紬??猰???勻涸??絶?倝??猰??屠鴽鳥蟟欰涸?搬絕卓?ざ?欰暟?涸呍?猰??炄僽?涸?玐猰??巑告????????僽欰暟?助???絆ㄤ禹絡(luò)欰暟?傞去涸????餘涸?駟?湡涸僽霃雦痗ざ叻?涸欰暟禹絡(luò)罜欰ㄐ禹絡(luò)?剣??涸練???霃雦涸??絁騟?銳嵳??玐?霚?????梡?霃?臘?玐霃雦???欽?玐??崵???撐練禹絡(luò)匬?涸霃雦-匬?-崵霚-???梠DBTL鷶幯??ざ?欰暟?涸呍?瘻殜??欰暟?鳴??DBTL??媯?梠?撐????匬??銳涸絈脅欰??ざ鷓涸????邍1.ざ?欰暟?霃雦-匬?-崵霚-??灇瑕瘻殜覆?涸?銳???助 餴俱勻彂俒柄?ざ?欰暟?傞????垷?絈蝒涸???渹絈脅灇瑕梡朐??劅?霃雦DesignDBTL???劍?剣濼霋涸?炄?黽??涸錞??欽梡剣涸叻??欰暟??????去靧鸑騟???絆鵳遤椚雦??務(wù)叻??涸欰暟????僽DNA宐??鶣涸欰暟??涸鵂玐務(wù)???銳????助???欰暟???去靧鸑騟???絆?絈脅??霃雦涸鯡??雦皾劼垷??區(qū)?匬?Build匬?鵂玐??DNAざ???晙媯絆鄳????綘鰳?DNAざ??助??欰暟?涸封?鵂玐務(wù)飾滿???銳涸佅丒?欽?割ず???的??銳?欽垷匣?湬?呏研DNA??鵳遤??ざ????DNA鄳灇瑕?プ??封?Gate?Start-Stop??呍薗?鵶???涸DNA絆鄳(OligonucleotidesmediatedDNAassemblyOLMA)瘝?貓絆鄳倰歲???綘鰳?助?痦???呍??(ZincfingernucleasesZFN)?痦?去碫鯪?慨崞??佪?暟呍??TALEN封??湡?涸痦?去CRISPR-Cas?助?CRISPR-Cas?助?剣佪檜??乼????佪卓?爍瘝?挿僽湡???綘鰳涸?崨?助???涸CRISPR-Cas9?CRISPR-Cas12a(Cpf1)??綘鰳?助???貓欰暟?禹務(wù)???岌?欽??割ず涸???渹絈脅務(wù)?欽涸匬?倰歲????????崵霚偽雿僽??盟デ???????RBS??鵮僽鶴鰳絁垷??涸去靧鸀??鸑鵂椚???椚?霃雦た??????涸瑲」???鷥湡叻?姼?佪??爍ㄤ絑崸涸唬崵倰歲?剒?欰暟???絆ざ涸鷥?蕘??銳??絡(luò)涸唬崵倰歲偽歲忘駈ざ?欰暟???????欰暟???鶴鰳絁騟?去靧?靈?鸀?絆ざ涸?寵湡??絑?霚?封?欽?貓?鸑??荈??涸癃鷥?唬崵?助勻??崵霚涸佪檜??????渹絈脅罜鎊?欽?鸑?癃鷥?助?鳥癃鷥?湱????臘??涸絈脅??蕘??銳?????匬?涸絈脅??鵳遤欰?崵霚??ざ?欰暟?DBTL涸霚??唬崵????銳??鳥?佪涸荈???媯??玐??????鳥??霚??佪?霆DBTL鵂玐??鳥獤??湡???繠?ㄤ薉?瘝?????匬????涸???玐25???涸??????跗涯餘猰?務(wù)?(?嵳)?娀憲欰暟?助灇瑕???瘝灇瑕劼匬???匬??湱?ざ?欰暟?荈?????湡???????絑?梡?荈???????鸑?鴪?300ˊ600?/d?垷??撐練餘磜絆鄳?鴪?100/d絆鄳姻爍檜>90%ず傞鵮?梡??貓垷?欰暟涸荈????乼????Learning??鵂玐??ざ?欰暟?DBTL務(wù)涸?銳?梠臘?????梠佖雦???????禹絡(luò)欰暟?倰歲涸絆??助鵳遤??-RNA-跗涯-去靧-邍?割ず??涸?區(qū)匬????-邍?ㄤ去靧靈?緸絞涸濼霋?靶瘝???鵯?鵂玐覆?僑研佐?侮ざ?僑研?區(qū)?絕卓?錠?ㄤ?垷?區(qū)瘝?灇瑕獤稡涸???垷??欰暟湱?涸僑研鵴??垷??欰暟告垷??欰暟湱?僑研涸?椚???????垷??欰暟????涸???佐?侮椚涸??僑研???欽欰暟???ㄤ??蘭臘劼???瘝湱??助鵳遤?區(qū)??匬?僑?垷????絆??涸去靧緸絞垷?ㄤ?絈脅垷?瘝?ず傞鵯?僑研?絛?饆?僑研涸劼?????僽帿???????臘???鵯??絕涸錞??絕雿????ざ?欰暟?DBTL???媯涸垷????用劌??佪礵皍涸ざ?欰暟???崨玐欽?割ず?渹絈脅涸匬?ㄤ?臘????玐?劥餘?????欰ㄐ?ㄤ????????礵爍??/?/佖???????倗?梡劌?涸導(dǎo)?騟??劌撐練涸去靧靈??劌?涸欰?佪檜?ざ?欰暟??嚋?ぢ??涸鯪」剒?銳?渤????助涸?倝?DBTL?ざ?欰暟?涸?????助??DNA?DNA??綘鰳?DNA佅丒?ざ?欰暟???涸鶛去??紩?DNA??崵??鸑鵂崵?霃??區(qū)欰暟呋劥涸??絆????佪??DNA崵?僽?梡ざ?欰暟??鳥封?涸?炄??鳥???ㄤ??絆崵?????剣?荈搬歲欰暟涸????鄳縨涸?????鵳匬?涸欰ㄐ禹絡(luò)涸?鳥唬崵ㄤ???Sanger?痦紵磧?崵?DNA崵??助?倗鶛去崵??劥??儑衼崵霚??鳥?????僑紩???DNA「???助???絡(luò)???玐?助?乼?涸?????僽勻荈荈搬歲?僽?梡?暟貓?????涸騗餿湡叻?????暟涸鷓欽?剣僈儑涸?????DNA???劌呏研?寵??ざ??????鳴?荈搬???蜦?倝?暟涸?臘?21?紬?勻?錞垷??礵???DNA??ざ?欰暟?涸佪檜????邍2.DNAざ??助暵挿?嬙?助?椚熧??腂ざ?歲?助暵挿?霴檜??????稇?錞垷ざ??劥鰍蓿晙ざ?歲??歲?餘鰍????ざ??欽?剣嫫霚???瀊???ざ??助???歲?餘?菚?暟瀊鰍??????????絆傍劍休???媯歏??歲?餘鰍????ざ????歲?劥霚??嫢?DNAざ?歲?梡?嫢??ざ?????DNAざ?皍?僒欽?DNA瑲」檜歲鰍?餴俱勻彂嬙齜僤?區(qū)??綘鰳??絆綘鰳?助僽??貓?湡叻??鵳遤綘鰳???涸???玐?助?銳剣??3貓?助??跗涯呍??ZFN?碫鯪?慨崞??佪???TALEN??CRISPR/Cas禹絡(luò)???綘鰳?助臘??ざ?欰暟涸暵DNA??鵳遤佖鳴???去?助匬?ワ劍?姿?籖椫??鵳遤???綘鰳匧????????欽?痦?去CRISPR/Cas9?助??儑衼匬?皍?倰???鷓欽?????欰暟???ZFNCRISPR/Cas9?〡?崞?欽???屛毫????ZFN?鵂??僑?渹絈脅CRISPR-Cas?助涸?欽?銳?彂??Cas跗涯?飾絈脅嫫????CRISPR禹絡(luò)涸?欽?鵯瑕?プ????欰暟務(wù)???????欰暟荈魧?彂CRISPR-Cas禹絡(luò)涸??絆綘鰳?助??邍3.CRISPR?助封??玐烵傞?絁 餴俱勻彂俒柄?StrategiestoovercomethemainchallengesoftheuseofCRISPR/Cas9asareplacementcancertherapy?DNA「??DNA?鵮偽歲湬??爍?ざ?????涸DNADNA晙媯鵮?饆?絆鄳?助鵳遤???DNA絆鄳僽?欽?絆鄳?助涸ざ椚????媯ざ?涸?呍薗?晙媯鄳???晙媯DNA鴪?????欨蕘??絆??DNA??涸ざ???欽涸?呍薗?絆鄳倰歲剣?貓鵶??絆鄳歲ligasechainreaction,LCRㄤ翸ざ?絆鄳歲polymerasecyclingassembly,PCA??欽瀊??晙媯絆鄳群蒀????絆??DNA??涸??絆鄳妃僑鰍?鵂玐務(wù)??涸???鷥ㄤ崵?瘝餘??劥??湱????姼??劥?荈??????僽DNA絆鄳?助涸勱勻封?倰ぢ?飾告?飾彂ㄤ封?歋勻???1910StephaneLeduc????syntheticbiology?ざ?欰暟??霒告?ゎ????涸ざ?欰暟???湱?欨鵴?2000???????綫?虹??眜俒畎封邍??助劍??Nature???鄄雩????ざ?欰暟?涸傞去???ず傞ざ?欰暟?涸封?猌割??鶣???助涸佅?鵳?????紬?勻DNA???綘鰳???崵?瘝?助佅丒滿??絁騟?玐?去靧?玐?屛毫?欽瘝?禹??助?涸?妃瑲灶?炄灇瑕?絯ぢ??鵳???ざ?欰暟?涸封??玐ざ?欰暟?遤???????媯?炄灇瑕蟟蔠劍??炄灇瑕?擿劍??欽?封劍?????餴劍ぐ劍?ざ?欰暟?涸?助??割倗瑲灶?欽薴??絯????1010?3去邍?卓??襧稇????肅勍蝒務(wù)涸ざ??4-7?玐?助鵳姿鰍綕???????玐?椚?傈幯帿?玐倰歲ㄤ???倗獤巤涸?媯?梡??玐欰暟?涸傍劍??暵挿?8-3?玐?媯鵳?姿?擿ざ?欰暟??康?欽??????倗??ざ?欰?岤?傈渤??Amyris???欽?嫢蝒吳休??欰??襧稇?4?玐???涸?霃ㄤ欰暟?僑研涸?彂?欽湱絕ざ??猰輑ざ玐?鵳?姿?帿餴劥?鳥??遤?鵳??鳥??劍滿?椕ざ?欰暟?涸??嵠惐??ざ?欰暟??ず傞鵳??鳥???媯??邍4.ざ?欰暟???封?涸去邍灇瑕?卓 餴俱勻彂俒柄?ざ?欰暟???欰ㄐ猰??翸灇瑕倝傞??務(wù)?ざ?欰暟?贍飾姿鰍儻告封?鴽鳥???????欰暟???呍??助??雿俒封邍ㄤ??歍霼僑????椕痦???僽?康?欽畮??????縨??????溏?妍繠瘝??湱嬙??封?ざ?欰暟????鳴?欽????劥??割?????研?椕?俱???遤嚸???湡???剒?涸ざ?欰暟???宨翸??錭??峸??磾度資割????絑封???絈???遤????佟瘻?餴劥鳥ざ?欰暟?????????剣幡裂??佪???欰瘝暵挿臘???????欰?梠?涸??剣劅??蚋????臘彂?勞俱??????瘝?????封欰??鳴垷?涸」??姼?「篩瘻佅??湡?ざ?欰暟??鵳??椕?霋?ざ??畊?涸?鳥封?傞劍妍湅?繠??務(wù)?瘝??/????猰封??篩瘻??ㄤ?殜??瘝?絶??鵳ざ?欰暟?封??鵛?勻?椕?銳??篩??絯??ざ?欰暟?湱???篩瘻??欰暟?猰灇ㄤ??封???鴽枡??椕?銳??湱絩?用ざ?欰暟?灇瑕務(wù)????麑??椕涸ざ?欰暟?灇瑕緸絞?繠??薉????涸封鴪???ざ?欰暟?灇瑕??封?鵳玐鰍??妍湅剒傍鸑鵂痦?灇瑕呥卹雦夠?篩瘻?????湡餴?涸倰??鵳ざ?欰暟?封?歲????瘝?プ???ざ?欰暟??湱??助???????劥?涸灇瑕封??殜?20122016?ざ?欰暟?騟絁??ㄤ?薉?ざ?欰暟??殜雦夠?僽???????鸑鵂騟絁?倰???ざ?欰暟?封?涸???繠????絶?勻??ざ?欰暟?涸封?荈20193???玐欰暟???去欰暟絑崸涸灇瑕騟絁????欰暟絆?玐??????玐欰暟??勞俱猰?騗?猰?倝灇瑕騟絁?????涸灇瑕騟絁??ざ?欰暟?僽痗ざ?焫湡叻涸?倝?助遤??封??「???篩瘻佅????絡(luò)?助騟絁湱嬙ざ?欰暟?鳴涸梠????絯??僈儑??欰暟ざ????L-?孽??6-APA?峫??寶瘝????焫??臘署???劥涸??暵?????篩瘻倰???973?863?挿?炄灇瑕封?雦夠?用?ざ?欰暟??????劍??????欰暟?鳴?助涸佅?????傞劍???ざ?欰暟?助??????」?涸?銻??助??2022?5??封佖??封????欰暟絑崸封?錞夠?????毫?????嶊餩?綁蒀?焫?欰暟??瘝?挿??封?欰暟絑崸欰暟絑崸?????劍????餘?封?涸??????篩瘻涸??佅??ざ?欰暟??鵓勻??銳涸封?劼麁??邍5.?椕ざ?欰暟???封?ㄤ?殜??怵鵳騟? 餴俱勻彂俒柄??椕ざ?欰暟???梡朐????涸?爙?1.2.201ざ?欰暟??餴涸?銳鯪?挿鵯??ざ?欰暟?杝錭?ZymergenㄤGinkgo湱絩???た勻罏???IPO涸?罌叻????ざ?欰暟?饋麤???2021呏研Synbiobeta僑研2009-2020??椕ざ?欰暟????盡?雦蜦輑餴?215?繠?罜?2021?鴪?180?繠???蜦輑餴??鵂?12?80%??邍6.2009-2021??椕ざ?欰暟???輑餴????涰?繠?餴俱勻彂Synbiobeta2018??勻??ざ?欰暟?輑餴割倗的??研?腡緸僑研2022ざ?欰暟?涸掚???絊??割??2022???ざ?欰暟????模?54飾輑餴僽?毫匣?輑餴妃僑剒?涸絈???????2021??傞?飾挿?2023????ざ?欰暟????103飾輑餴?300?瀦?去邍??覰兪?欰暟20221?8.7B3202324B4蕘姼?盡涸B?輑餴??19?餴叻涸倰ぢ???涸?餴?銳翸送?欰暟?蚋???欽ㄤ??欰助?輑餴?媯??〡?涸??ざ?欰暟?姻??封???劍????銳???劍?媯?姼輑餴鯫妃湡??務(wù)?AA?2??絑模?輑餴涸ざ?欰暟?????霣欰?幡??銯廩?務(wù)猰?暟??帿??鵳?瘝猰灇?吥????ざ?欰暟???屠?卓????????鵯??媯??割?猰灇?吥?絑????擿絑??贍搬ざ?欰暟???湡??霣欰??饋欰暟???欰暟瘝????告侮?僑?鵮湱?鰍???滿饋麤????助鶴鰳鵳?姿?擿?呍???鵳?姿休??釁??劍?剣劌??盡封??遤?杝錭???邍7.2023?ざ?欰暟?饋麤輑餴??去邍??輑餴傞?輑餴??輑餴錞垷?餴劼匬??皍?馰?欰暟2023/7/11A鯫鵛??欰暟???ざ?剪??ざ?2023/7/06Pre-A鯫??ざ?欰暟?助灇??鵴欰暟2023/6/30貓?鯫僑????兪???語菗???助????欰暟2023/6/25B鯫鵛2??FDCA?ㄤ欰???CellX2023/6/16A+鯫僑??????殜?餴倰僅鵳欰暟2023/6/06A+鯫-ㄤ兌欰暟2023/5/30??+鯫-??餴劥?呔餴劥???倝ワ?勱勻2023/4/26A+鯫僑????僈??勱磧欰暟2023/4/25Pre-A鯫僑????倝????綘鰳ㄤ欰暟肫貓????欰ㄐ2023/4/20B1鯫???GreenFuture???欽鯪????梐欰暟2023/3/21Pre-A鯫鵛??綁崍餴劥???ざ?欰暟ざ欰猰?2023/3/21A鯫-褐暋????ざ?欰暟灇?蕯?欰暟2023/3/18Pre-A鯫?????倝?餴劥???絆聘嫫稇蚋暟??匧?欰暟2023/3/8??+鯫????絶餴劥絈脅??聘??餫?欰暟-僑???ざ?欰暟?欰暟勞俱??????2023/2/20B鯫餛???饋欰暟??AI跗涯餘霃雦????覰兪?欰暟2023/02/14B4鯫4??務(wù)?餴劥??務(wù)?貓彂-貓?鯫??紩紤勒務(wù)?貓????卓???匬??2023/02/02A+鯫3.95?????饎??2023/01/16Pre-A鯫僑??-嗃暟???灇?餴俱勻彂?ざ?欰暟?ワ???暟?臘?助??碫?盡?DNA&RNA湱?????崵??ざ????綘鰳瘝????ㄤ鯡?剪康???碫?盡?銳???蝒吳涸癃鷥?佖鳴??????封瘝傋???欰暟?霃雦ㄤ荈??瘝ざ?欰暟????康?僽?欽????盡?ぢ??絊畮??欰???僽湡??僑剒?涸?碫?????康ざ?欰暟??臘?助?盡?康涸ざ?欰暟??臘?助?盡????崵??ざ????綘鰳瘝??ㄤ鯡?剪??欰暟??匬????助佅丒??????助??涸盡?剪?灇封鵂玐務(wù)獤稡???涸DNAざ??欰暟??霃雦倰?涸絑?封??僑研?臘??務(wù)康??康????劌皍???爍涸剪????Biosciences?DNADesktopGeneticsCRISPR?務(wù)康ざ?欰暟?????盡ざ?欰暟????務(wù)康?ざ?欰暟????盡????梡蝒貓涸鷥?ㄤ?僽ざ?欰暟遤?封?涸?????ざ?欰暟??盡?岤?蝒吳佖鳴鷥瘝欰暟ざ??助ㄤ?蔞灇封?荝??匬?荈??????欰?鯪?涸ざ?欰暟??????GinkgoBioworks鸑鵂???佪涸荈????匬???欰暟?鳥佖鳴涸?助??屎?MetaMixis?盡?霃雦欽?封梡欰?ぐ貓???涸倝鸀??助???灇封?プ臘?劌????欰暟鵳遤綘玐?欰?欽??礵??俱涸??暟餘?????盡鸑鵂??康????休??ざ??鳥ざ?欰暟??????鵳玐??康ざ?欰暟??欽??盡ざ?欰暟?????康?????盡??????康???欽?????盡?欽ざ?欰暟??助欰?割ず??涸湡叻????絡(luò)瀖??ざ??助鵳遤?邉??剏去?ざ?欰暟?涸?欽?????蚋?????勞俱?臘彂???ㄤ??瘝???????????務(wù)?梡?ざ?欰暟?涸?焫?蒜臘?絑崸涸綁蒀??暵??歲涸?????PoseidaTherapeutics?欽??綘鰳?助??????艑濃瘝氬氻涸屛毫倰歲?Antheia姻?佖鳴?嫢?袕蟎祪鯪???晙碫蚋暟?臘??祪ㄤ?????GreenBiologics?欽??磨ざ??幡峇?ㄤ?俱涸???欰暟敍俱姼碫??姻?鸑鵂?鸑?偽?涸?欰暟霃雦?倝涸??欰臘彂???LanzaTech???欰暟臘???孻?焫?歌掬瘝?孞鯪??敍俱ㄤ????絑?絶棇菔瑟ざ?欰??焫?孞敍俱???鵳遤?玐佖鳴????暟蠒???涸嶊?檜ㄤ?欽檜?tSensory????暟????賑?ㄤ祪???嶊餩?姼碫?????淼??撐僈絕匬瘝嶊餩?涸???????Meadow搬た?臉?跗涯?絓??飾?欰?淼????ㄤ?俱姼碫????姻??僈ざ???涸欰?ㄤ??鵂玐AvaWinery?崨遤袕蟎?涸????勻???ゎ袕蟎涸ざ?袕蟎????俱霪??鵮??嫢ㄤ桪磧鵳遤?玐佖鳴袕蟎?涸?麤???ㄤ孞???邍8.ざ?欰暟?休?????????康 餴俱勻彂CBInsights?嬙齜僤?區(qū)??ざ?欰暟???涸?????鰍模侮?務(wù)?康??剣???????康???銳??ざ?欰暟????助去邍???????鰳??屾鴽猰??空猰欰暟瘝????務(wù)康??????涸垷?侮ざ猰灇????爢?ぐ歲涸?助餴彂鸑鵂荈???劼???????欰暟僑研涸宨翸勻??灇瑕涸封梡?鸑?ㄤ???罜匬??助灇封????康??????ざ?欰暟?涸鍒?倰呩去邍?????ㄤ欰暟??叕蜟欰暟???欰暟???欰暟?遙鵳猰?瘝?????康???岤???欰?鵂玐務(wù)?欽?鵳涸欰暟?玐?助確程?佪涸欰暟ざ?倰歲?ざ?欰暟?欽?欰暟?蚋??????????勞俱瘝??去邍????Gikgiookss?208歋?溁椚???MIT侅?TomKnightㄤ???MIT蜦?????涸Kelly?ReshmaShetty?BarryCantonAustinChe49GinkgoBioworks????????150??GinkgoBioworks??ざ?欰暟????涸務(wù)康盡???蝒吳佖鳴?荈?????呍?鵶?????康?助??盡??涸熾??????????康???欽涸??綘鰳絈脅??鳴?Foundryㄤ去瀦?Codebase?銳????????1?玐?欽??鯡??劼??荈???僑研?區(qū)?助呏研???寵鵳遤絈脅綘玐?2欰暟????呍?唬崵??ㄤ剪?欰?佖鵳剪??湡??姺GinkgoBioworks??鵳遤????????20179GinkgoBioworksBayerJoynBioざ??封倝??宆?欰暟?20214GinkgoBioworksCortevaAgriscience??欽ざ?欰暟??封?倝涸嗃暟???助湡?霪ざ?涸呍?僽???勻??暟貓ㄤ??剣?欰暟?????201610GinkgoBioworksCargill鴪?ざ??靂?????封?欰暟蝒吳?201811GinkgoBioworks?盡Glycosyn??封???翸祪HMO???肫??肅麤?欰暟纈????20192GinkgoBioworks?9000?繠??用MotifWorks?岤??欽?欰暟????去跗涯餘ㄤ?????封?割ゎ?暟??涸???俱?鵳?嗃暟聘ㄤ剏去???????蚋??20189GingkoBioworks??椕剒?涸??????盡CronosGroup???????鴪1.22?繠?涸ざ?雦夠???貓杝暵涸???ざ暟??蚋遤?????涸屛毫/?蚋嶊餩??20196GingkoBioworksRoche1600?20196GinkgoBioworksSynlogic8000?餴?鳥?鵳?玐渤欰蝒?倝毫歲灇封?2020GinkgoBioworks?Concentric???COVID-19??梠?屛椚??2020?10剢GinkgoBioworks?餴4000?繠??用?封臘?倗????涸?欰暟???梠?務(wù)涸??寓群暟??邍9.GinkgoBioworks??歘? 餴俱勻彂GinkgoBioworks?嬙齜僤?區(qū)GinkgoBioworks?2023?2.75???務(wù)?玐佐?蕘??1.75?繠?欰暟????2023?佐?蕘??1?繠??姼?Ginkgo2023??Foundry?100?湡2023Q1??13?湡?Ginkgo休??涸???銳????助???涸呍?畊??????荈???助???鯡?ㄤ僑研?區(qū)?助???欰暟?鳴??梡?欰暟?玐涸荈??ㄤ錞垷?霃雦ㄤざ?湡叻DNA???絈脅鵳遤崵霚?癃鷥?剒?佪涸蝒吳瘝乼????欰暟?鳴??佪模??鸑鵂侮ざ欰暟?鳴?????僑研ㄤ??僑研?Ginkgo???麰?去(Codebase)?ざ?欰暟??康霃雦??僑研佅丒?Codebase??欰暟??ㄤ霃雦???垷??涸??晙媯ㄤ?鄄?撐?欽涸?渹蝒吳????倝涸欰暟??湡崵霚ㄤ?倝霃雦倝涸ざ?欰暟?Foundryㄤ去瀦?Codebase????湱鰉湱????Ginkgo??欰暟?鳥匬??涸??????邍?邍10.GinkgoBioworksざ?欰暟???荈??涸Foundry??崨玐+Codebase餴俱勻彂GinkgoBioworks?嬙齜僤?區(qū)蘭臘??鳥展?欰暟?灇瑕?去CRISPR/Cas9??綘鰳?助????ぢ鵳?瘝???助涸?岌?欽??佖」????綘鰳DNA?DNA????7跗涯餘絆?ㄤ去靧絆?涸?鸑???崨玐」?餿勻餿?欽雮??臘????勱剣涸鳥??絈脅鵳遤欰暟?玐佖鳴?告欰ㄐ???ざ?欰暟?涸灇瑕?韌?剣??????崞??絁??割??崵瘝暵?????鵳遤礵??崵ㄤ霃雦僽ざ?欰暟???涸呍????劼???????涸?崵臘?贍搬??臘??湡叻絈脅鵳遤?劍涸DNA佖」告??偽歲?崵欰禹絡(luò)涸DNA鄄佖」た涸邍??ず傞?跗涯餘?ぢ鵳?務(wù)鷓欽?厤?暵??兞?????ぢ鵳?崞?涸???臘??鯪獴〥?貓湡叻跗涯??荝鵯?倝?助割??鸑欽??姼???緃???錞垷??務(wù)?倗?錞垷遤?涸臘??????玐??????櫕?僽霃雦臘????????絕卓1-100?的??休?錞垷100-10??涸封?禹絡(luò)?鵂?剣??勱臘????饑???休?欰?涸?餕呩??偽歲?梡??鵯割??勻???涸絑崸??鵮?荝霪??た絯涸?餴?岤??????劼??????割?銳霫絈椚鍒劼?涸??????俒?鶣?務(wù)?銳涸?崵臘?鸑鵂劼???垷??雲(yún)絅僑研涸?絆鱈????鵳遤絡(luò)雦??涸翫禹????剣???涸?霃?邍鴪???剣涸?禹?劼????欽?霃雦ざ?欰暟?禹絡(luò)??欽???邍錜麰??騟?罜?梡劌珘?涸?騟?荈???助?霆?jiǎng)噥莺詣???劼???涸?鳥封?勻彂?傈渤??涸雦皾臘?ㄤ??僑研?涸??荈??臘??欰駈?僑?ㄤ餘?涸僑研勻雲(yún)絅剣佪涸劼??湡?雽?灇瑕???侮??禹絡(luò)???????晙媯靧緸絞?靈?緸絞欨蕘僽絈脅侮?鵳遤??涸霃雦?ざ?罜??割倗霚?涸??垷??荝?湡涸灇封ワ劍???餕檜??搬罜??僑邍?僑研僽?ず?????務(wù)?欰ㄤ鵳遤?區(qū)涸?盜剣倝涸封?告鵮割駈?佅?劼????ず傞雽?????僑研?割忘駈劼???剣佪涸銳寵??緃?叻??涸僑研佐??姼?佐?倝僑研傞??罌贊?鵯???劼???涸皾歲??鸑???佪檜?荈??涸?助湱絕ざ?罜??嵳?涸剣佪僑研??雲(yún)絅垷??劼???ㄤ荈??湱絕ざ匬?DBTL鸑鵂侮ざ荈??霃??蘭臘???鯡???僑研盜椚禹絡(luò)匬?ざ?欰暟?荈??鄳縨?梡?欰暟呋劥?》?DNA??絆鄳?癃鷥?????暟唬崵涸?崨玐荈??乼???????佪檜ず傞鄳縨?欰涸???餘?僑研絕ざ???助剣劅?鸑????劥???梠??梡ざ?欰暟?灇瑕務(wù)霃雦-匬?-崵霚-??DBTL涸荈??鵘遤1??務(wù)荈??巊??椚??畀??崨??????瘝?鳥?ざ?欰暟???炄?霽??欽??涸灇瑕佪檜???剣?岌鷓欽?涸欰暟??鍒?倰呩??邍11.劼???ㄤ荈???割ず涸倰?佖鵳?劥涸ざ?欰暟?崵霚-??(DBTL)?梠?荈????鸑鵂?zhēng)g??椚??畀ㄤ?崨?????霃雦??鯫鶛去涸錞?勻???梠務(wù)涸嫦?姿?? 餴俱勻彂UIUC蘭臘??ざ?欰暟??涸?欽-劼??劼????ざ?欰暟?務(wù)涸?欽匧??岌割?yuàn)??霋??跗涯餘絕匬ㄤ?臘?崵?跗涯餘?ぢ鵳??去靧鸀?匬?瘝ˋˋ????僑研務(wù)?》騟?垷???欽?倝僑研?涸??????欽劼??欰暟???務(wù)劼?????鸑鵂雲(yún)絅皾歲??用垷????暵??》ㄤ?碫罜剣佪??僑研涸鍒靀佪卓??????絏劼????跗涯餘?ぢ鵳?務(wù)涸?欽??湱?蘭臘????挿?跗涯餘?ぢ鵳?鵂玐爙?劼????荈????絕ざ涸?跗涯餘?玐僽ざ?欰暟??銳?欽??罜?ぢ鵳?DirectedEvolutionDE僽跗涯餘?玐務(wù)剒???剒??涸????????MachineLearningML?跗涯餘?ぢ鵳???涸?梡雦皾劼霉?」????劌剣佪涸?ぢ鵳??荈???助?梡????歲??助歲務(wù)?鳥?遤?傞?涸?撐練涸???罜蜦??鸑?涸剣佪僑研???鵶絯跗涯餘鵳??荝覆?????姿??欽?呋劥?崵?霃雦??」??鸑鵂?荈????匬??邍鴪ㄤ癃鷥??」??欽癃鷥?涸?餘?僑研勻雲(yún)絅劼???垷???崵鷓??呏研?崵鷓??涸絕卓霃雦?た?鯫涸??」??鶛去?遤??姿?湬?霋??剒?」???邍12.??鵶絯跗涯餘鵳??覆?涸姿?嚋鶣 餴俱勻彂CellSystems?欽?呋劥劼???垷?霃雦??」???ぢ鵳??欽?ぐ貓???呋??????劼霶」????勻確程跗涯餘」?兞錜?霋??剣??暵?涸」???劼?????涸?ぢ鵳?MLDE?癃鷥?割?僽剒?涸???稇罜僽?銳剒???????鷓??」???欽?呋劥?崵垷?霃雦????姼??呋劥??滿??跗涯餘ㄤず彂跗涯餘涸?欰?????偽霋?歋?鵳遤?崵涸??碫?割ず????涸?呋劥垷????貓?鵳?ㄤ??鵳?垷????鵳?垷???EVmutation垷???欽?ゎ僑???跗涯餘??涸????僑研??割?湡叻跗涯餘ず彂????僑??垷?僽露鎊垷???鵳?錞垷?垷ESMㄤProtTrans??邍13.???欽?????鵳?垷?涸?呋劥」?佪??崵?勻霃雦濼???垷?呏研?剣跗涯餘?偛涸??鵳遤雲(yún)絅??垷?呏研???嬙??倗?涸婎???涸湱匹?饆?勻雦皾嚋檜? 餴俱勻彂CellSystems?封?崵」?暵?涸劼???垷??匬??邍鴪ㄤ????」?俒?た?蜦?????邍???劼???垷?涸雲(yún)絅僑研?皍?涸絁????劌撐練涸牟絑緸絞??崵勱濼」?涸???歋姼?絡(luò)?ぢ鵳?務(wù)鰍?餩???銳鶛去涸?鸑?癃鷥???欽劼???務(wù)」?兞錜涸?崵勻去剏???銳崵霚??」岌????」???挿瑲」?雲(yún)絅涸劼???垷?????蜦?挿瑲????邍14.??雲(yún)絅涸??露鎊垷?蜦?涸????欽?邍爙」????鸑鵂ず彂??鵳?姿?靈霪?????暵??歋姼蜦?涸」?鷓??僑研?欽?雲(yún)絅??垷??皍?涸絁????劌撐練涸牟絑緸絞? 餴俱勻彂CellSystems劼???雲(yún)絅?銳??僑研罜?鴪??爍涸?崵絕卓???涸僑研??剒鵛涸灇瑕饞????岤雲(yún)絅爙?僑?剣???100?涸?N?兞?N霃縨?灇瑕?プ割?銳鵳遤?鷥??」???N-劼???垷????呏劥?鼙???鸑?癃鷥ㄤ鷥?倰歲涸?寵鵯貓??崨玐?鷓欽??剣跗涯餘?玐???鵛?勻ぐ貓ぐ呋涸劼???垷????皾劼猰?歲荈搬露鎊?椚NLP???Doc2Vec??歲?瀊劍雵?mLSTMㄤtransformer?務(wù)?崨NLP垷??欽港漛僑研勻????俒垷?涸?臘割?偽?ぢNLP垷???倰歲?垷???姼?????涸垷??封??跗涯餘?垷涸欰暟?暵??鍒?倰呩傞?靭??欽?崨NLP?〥?鵛?勻AlphaFold?RoseTTAFoldESMFold?崵??蜦???劌?僒蜦》歋姼?欽絕匬??涸倝?僑研垷??????剒?鵳涸助?ぢ??倝涸宐??梡劌?N?呋劥?崵??欽MLDE??跗涯餘涸???僑???僑??孽??割瘝????剣20貓??欽?餘?」?鷓??涸劼???垷??割?臘模侮確程侮?涯餘兞錜??癃鷥鷓ざ?ㄤ崞??傞????罌贊?剣鰍??遤?涸」???貓?欽涸?ぢ鵳?倰歲僽?挿?ㄤ霶」??銳絕匬垷?務(wù)?臘涸?挿?唬叅?剣20?孽??ㄤ帿?瑲」????癃鷥ㄤ鷥?倰歲罜「????鵛劍灇瑕?プ?欽倝涸MLDE倰歲?欽ぐ貓帿?欰?垷?勻銻渷劌?岌涸?????鷥???臘????瑲」」???邍15.??鸑鵂ざ?帿?欰?垷???瀦露鎊垷??雦皾劼?ぢ鵳?????跗涯餘??瑟た?貓倰歲??剣佪確程??兞錜罜帿?欰?垷????呋?/??瑲」涸?臘」????確程??涸跗涯餘兞錜? 餴俱勻彂CellSystemsMLDE???瑲」勻鵳遤涸告絕剣?絶???呋?涸?臘跗涯餘?????兞錜??劌????欰垷???雲(yún)絅僑研務(wù)??悶?涸?絶???た???呋齡?鵴猌?欰???涸倝?????鵳?鳥?劌???劥劌??皍??僒?爍?た絯??崵?涸霃雦??紩??罌贊た絯??崵?涸霃雦僽??鵶絯跗涯餘鵳?呥卹務(wù)涸銳姿??鸑鵂霃雦??蜦》?僑勻??剣」?鵳遤???爍??鯫垷?雲(yún)絅務(wù)割倗鵳?涸??絑?欽?呋倰歲?饜尺倛????倛鵂玐勻蜦??皾?崵?帿???垷?ㄤ饜尺倛??涸絕ざ僽跗涯餘?玐涸呍?瘻殜???罜確程劼???務(wù)涸倰?????臘僽???剣???告剣馰涸勱勻?yàn)氳挙??封荈?????佅?俒????跗涯餘邍鴪ㄤ?區(qū)癃鷥??ぢ跗涯餘鵳?涸弳???務(wù)???涸倰?模?告導(dǎo)撐涸??霚??荝??ワ劍???餕檜??荈????????椚?涸??梠????欽??鸑?僑研欰????ㄤ?區(qū)涸熾?ㄤ鯡??罜臘??鸑??皍???撐涸??崨玐???ワ鯪傞??儑衼蒜溁???劌??佅?劼????涸俒????跗涯餘邍鴪ㄤ?區(qū)癃鷥????欽務(wù)昶????絆?鳴?ㄤ??霿???鵳欰暟?鳴欰暟務(wù)?iBioFAB?封?荈????崨玐嫦ワ???遤僑?妃DNA絆鄳?iBioFABㄤ劼???涸?BioAutomata???梠禹絡(luò)欽?騟??玐????欽??た?????務(wù)霫絈???16.鵶絯MLDE?剣?????鸑鵂欰暟?鳴??梡荈???劼???湱絕ざ???梠跗涯餘鵳??餴俱勻彂CellSystems17??欰暟?鳴?涸???ㄤ?????鸑鵂靈?鯡?鵳遤?餴俱勻彂CellSystems?荈??涸錭?SDLsSelf-DrivingLabs?荈??Experimentation???荈??嚋?涸???勱勻??涸饞??SDLs暟?務(wù)ッ梡?杝暵涸劼麁ㄤ???盜鳴???傱餥告臘?梡??涸ざ?欰暟?鵳姿鍒?齡?欽梡剣涸倰歲??鍒?涸欰暟????SDs??研佐????蘭臘湱鸑鵂?椚鵯?僑研勻????絆銳鵳遤涸???????勻銳崵霚涸?霃?絕雿??邍18.SDLs??崨玐?爙 irect涸SDLsDNA???荈靈?暵?去靧鸀?涸」??ざ???涸去靧?暟????鯪??務(wù)??肅勍蝒???脅蝒??嫢瘝ず傞??AI?虛?空湱絕ざ?空?欽鵯???僑研??靈?割ず去靧鸀?涸」??罜鴪?湡叻?暟??剒??涸湡涸?AI?空??涸」?ㄤ??綘鰳?霃?歋????梠務(wù)涸荈???崨?蓿晙鵳遤崵霚?SDLsSelf-drivinglabs涸嚋?銳寵???務(wù)涸ぐ?梠蒜?歋荈??禹絡(luò)ㄤ??蘭臘勻模?????雦夠涸?????涸?遤?僑研涸佐?ㄤ?區(qū)瘝罜割?銳??鵳遤??涸???乼??告????助宐?罜鎊?臘偽歲鴪?模?荈?涸玐?????鶣??涸?助臘?慨?梡剣?禹鷶姿封??荈?涸禹絡(luò)???????碫?導(dǎo)鯊荈???嚋?涸荈?瘝紩????欽錭?溏儑爙???????絑???爙?梠涸霃雦-匬?-崵霚-???梠??邍19.SDLsぐ紩?涸????涸霫絈?? 餴俱勻彂ScienceDirectSDLs3?務(wù)荈?紩??鶣?霪禹絡(luò)?????杝用玐??0?剣??霃雦??遤ㄤ僑研佐??歋???椚?1?撐??????絛劼?????荈??霃?????椚嵳?涸僑研?3嶍??霃雦-匬?-崵霚-??梠SDLs?區(qū)涸??ㄤ叻雵?????歋???椚?4紩嶍?荈??劼???靂涸?遤ㄤ?錞僑研?區(qū)?Adamㄤ?s?5?sざ?欰暟?涸荈???梠?鰈??務(wù)?欽歏?ㄤ歏?瘝猌侔???鳴剣??湱???姼鵯???涸蘭臘?鳴?蘭臘??椚?鄄???ざ?欰暟?灇瑕務(wù)?皍?勻霹??鵳欰?霃??欰?絁蘭臘?鸑鵂?鵳ぐ碫痗ざ欰???涸蘭臘鄳??巊??椚??畀?PCR???叻??荈???盲?猌?劼瘝?用?????涸鯊?紩蘭臘欰????務(wù)?劼唒苮??鷐???礵??鳴?併??鳴臘??劌帿?勻霹僽???匹翫??椚?鸑鵂匹翫緸?????錞垷?????垷??倝??忘駈灇瑕罏????寵涸ず傞?梡欰ㄐ?涸鵴玐?????霃雦?錞垷絑崸欰??霪碫欽?ざ?欰暟?灇瑕涸荈??霃倶僽?玐???涸呍??鄄獦?欰暟?鳴?BioFoundry?蕘??姺3?絑餴?6???ざ?欰暟?灇瑕務(wù)?????蚚??傈劥?倝?????千瘝???稒?騈鵳??ぐ?灇瑕務(wù)???助劼匬務(wù)?菚???剣欰暟?鳴???呍??鵯?ざ?欰暟?荈??霃倶??傁欽??鳥?助灇瑕????封???搬雽???????荈?涸荈??霃倶???盡?GinkgoBioworks?盡?Zymergen?盡?Transcriptic?盡瘝?鵯?霃倶??涸錞垷割??臘??僽??灇瑕?プ?暵?涸??絁騟霃雦荈??鄳鲿?崞絈脅務(wù)?鰉??鸑?崵霚???崨玐????撐霃雦-匬?-崵霚-??涸?梠勻絆絉??梡?玐?涸嵳?霚???邍20.?椕?助劼匬涸濼ぜざ?欰暟?荈??霃倶?? 餴俱勻彂??猰?????務(wù)繠????紩灇瑕雦夠?DARPA餴?涸欰ㄐ?鳴?Foundries雦夠僽?倶剒傍?錞垷剒?涸雦夠??20115剢???勻?稡雦?縭絑餩鵛4?繠???倶?勻》?涸?銳鵳???倝涸ざ?欰暟?雦皾劼鯡?禹絡(luò)霪鯡?禹絡(luò)?ざ?欰暟?霃雦傞???1?剢綫瀊蕘1??臘?梡畮?畮涸港?匬???錞垷??緸絞???炄?姿?霆?dú)A暟?鳴涸姻ぢ?玐臘??用??錞垷DNA絆鄳倝倰歲???爍鄳?涸DNA晙媯僑?姼?剒?10????20?涸宐?????1/4??貓倝欰暟??涸霃雦??玐?欰??鳥7.5??絑?梡????孽??ざ?鸀?涸霃雦ㄤ???欰ㄐ?鳴?雦夠贍?》????銳鵳??遤?????姿??玐??欽???霽??挿??涸剒??助?????濼??絕匬???鳥佖鵳?厤???絕匬偽歲ざ??倝??絕匬??霃雦瘝???????屠確程?媯?勱勻?傉》?瑲灶?儑衼??梡剣?鳴臘???俒???椚???絏?椕ざ?欰暟?灇瑕?剣去邍?涸荈?????欽欰暟?鳴?Biofoundry?荈?ざ??鯪?暟鯪?慨崞??呋佪?暟呍??TALENs僽?貓?剣?岌?欽涸??鰳???TALENs??翸???鵳遤DNA???ざ???TALENs?剣?貓?絡(luò)倰??貓僽??杝涸鲿??邍鴪〥?貓僽?杝ざ?搬た千???ず?鲿??告鵯?貓倰歲?割?雽?鸑?涸匬??錞垷?欽涸TALENs??HuiminZhao瘝?????貓?姿絆鄳倰呩?P2A荈????涸??????鯪?呔?ざ?ㄤ邍鴪??TALENs?ず傞???封??貓?鸑欽欰暟?鳴?務(wù)??TALENs涸?荈?????欽劥??涸禹絡(luò)ㄤ倰歲嫦?臘??ざ椚涸勞俱?劥?餛鵂96.2%??檜ざ??涰?TALENs?TALENs???絆灇瑕??欰ㄐ猰?務(wù)涸雽????欽????????梡TALENs涸?錞垷?欽???癃鷥HuiminZhao瘝???荈??TALENs涸ざ?鵂玐?搬罜梡剣涸???????厤?暵???鵳遤?帿??????倝?縨??卓匬??欽?TALENsざ?涸帿???禹絡(luò)齡?鵵勝??佪?絑崸涸湡涸?剒絊HuiminZhao瘝??iBioFAB?模??絆鄳???iBioFAB歋絆????荈?劼????ㄤ垷??涸雦皾呥卹絆???霃?嫦?霃?餏餓??乼???割ず霃???歋劼唒苮鵳遤鵶??獴巊ㄤ??垷??鸑鵂??劼唒苮鵶????鵂玐垷??DNA絆鄳ㄤ鯪?搬た鵳?姿絆絉?鸑騟匬?ㄤ??絆?玐瘝??崨玐?邍21a?b??姼??鵮?封???侮?靈?玐?勻?靈??乼???雽???崨玐鵳遤??綘玐?邍21c??邍21.iBioFAB禹絡(luò)嚋鶣??鵘?絈湡iBioFAB涸熾???剣??劼唒苮5磧涸鲪麤??務(wù)??ぢ劼唒苮欽??????鵘鱈?劥〥??ぢ劼唒???巊??椚??畀?獴???呋劥iBioFAB涸???妃絕匬鵂玐垷?僽?禹絡(luò)??GUI務(wù)?封涸?iScheduler餏餓??崨玐紩?涸??膃劥欰???巊??椚GUI欰?獴巊騟絁崨玐垷????鳥?倝絆ざ?絆?割ず涸??崨玐? 餴俱勻彂ACSSyntheticBiologyiBioFAB涸?縨???遤鸑欽涸荈?DNA絆鄳??崨玐?GoldenGate倰歲???鳴ぐ貓DNA匬?????皍?崨玐HuiminScriptGenerator??????霃雦????荈??絆鄳鯪??幊ざ????DNA?涸???玐?膃劥欰???た?iBioFAB欰?劼??ㄐ?勻?遤撐練涸獴巊????剒?玐????エ?ㄤ傞?嶊署獴巊騟絁?絑鵂??????ず??勞??臘鵳遤絆ざエ》姿??た???????湡涸?エ???????鯪渹鄳鲿?巊??椚??畀?鸑騟????崨玐僽?欽iBioFAB?倶涸iBioFAB?欽??鸑?TALENs???嫢??絆?玐?iBioFABDBTL?梠涸?剣倰???梡?荈????嫦?鯫鵘遤務(wù)鷥?46?挿鵳遤霉???崵霚鷥?涸僑??????絛iBioFAB靈?鯡??搬た鯡?????務(wù)エ》銳絆鄳涸姻爍????欽Gate絆鄳勻絆鄳?zhàn)N磜搬た???欰暟??撐務(wù)崵?鵯?挿涸????絕卓涸???鵥?絛皾歲?雦皾???銳霉?涸挿?饜尺倛??皾歲?????確程侮?兞錜??た??鯫務(wù)鷶幯割???蜦?劌?剣?兞錜涸????暵???涸確程??痦?鯫務(wù)?????確程罜痦?鯫務(wù)涸挿侈???鄄雩???剒?涸暵?????鵘遤涸??霆僈騟???涸た?鯫?剣劌?涸????ㄤ劌?涸剒??鵯儑爙?饜尺倛??皾歲??た嫦?鯫務(wù)??劌?瑲」?涸剣佪?BiAtota?ia?皾歲僽?模??涸欰暟禹絡(luò)勻霃雦涸DBTL鵂玐?霪????霉?割?1%涸?臘」?嬙?劼癃鷥?爍檜??77%??務(wù)???崵垷?ㄤ饜尺倛皾歲鷥?歋??霿???鵳欰暟?鳴欰暟?鳴?iBioFAB鵳遤涸???BioAutomata?鍒??渱????鵯??????涸???劥?傱?乼?撐練劌剣馰涸僽??割?饆??欰暟劼?涸?岌??濼霋??鸑鵂??絆?涸荈??ㄤ??勻?遤?鯫霃雦?匬??崵霚ㄤ??DBTL?梠??剒?涸?????梡霪?梠涸鶛去???BioAutomata???遤????區(qū)僑研?鶛去倰?????涸欰暟鵂玐?BioAutomata?欰?涸??欽涸僑研?雲(yún)絅嚋檜垷????銳霉?涸??瑟?涸剒?挿禹絡(luò)佖鵳涸挿?霪??呥卹??鷓ざ???渱?僑涸剒???罜BioAutomata兜鷓??欽?騟???涸悶????????荈????遤?》倰歲??銳嬙欰暟靀匣?劌撐練涸霃?遤??區(qū)/歲??孞湱蒀靶-餘靶GC-MS??巊湱蒀靶-餘靶LC-MS??鵯?????剪告???饆??鳴劌?錞垷?劌撐練涸欰暟?鳴??侮ざ????鷥鸑?僽??欰暟ざ???涸邍鴪?鸑鵂絕ざ饜尺倛??皾歲ㄤiBioFAB荈??禹絡(luò)????欰?佪?涸蝒吳?霪僑研?涸嫦?挿?邍絛?嫦???涸暵?邍鴪宐?????????涸????姿霃雦ㄤ霃縨模??た灇瑕?プ涸錭蒀???涸??罏鯪」?禹絡(luò)涸港漛罏雮皾歲勻???霃雦ㄤ?遤??湡涸僽雮灇瑕?プ??涸湡叻?僑鴪?剒????邍22.侮???崨玐霃縨???僑霃雦?」?涸??瑟?鸀?絆鄳務(wù)涸???」????湡叻?僑たBioAutomata鷥??????雦??勻剒?涸????遤鵯??欰?僑研??務(wù)?呏研倝??涸僑研劌倝??崵垷?搬た?????遤????鴪?欽?霃?涸湡叻ず傞??????僑?ㄤ?湡?劥? 餴俱勻彂NatureCommunications?荈?皾歲????BioAutomata??涸痦?姿僽爍?禹絡(luò)涸??霃雦?鱈?ㄤ鱈???湡叻??霃縨た?鷥??崵垷?ㄤ??瘻殜勻?雦???欽僑研涸??鷥????銳霉?涸挿ㄤ銳?遤涸???鷥?禹絡(luò)涸?剣銳稇??霃雦???瘻殜???霃縨?僑研??ㄤ?崵垷?たBioAutomata??蜦》瘻殜鷥?銳霉?涸挿???勻iBioFAB?勻霉??鷥挿涸鷓ざ???僑研鵥???崵垷???霪垷??呏研爍??崵垷?ㄤ僑研??瘻殜歋?湡叻僽???渱?僑涸剒???姼?欽饜尺倛??皾歲皾歲僽鍒?姼碫??涸椚?鷥???匬????嚋檜垷???欽霪垷?勻????姿霉?涸?縨?罜????剒????涸??鯫僑皾歲罌贊?嫦妃霉?涸?劍絕卓???霪?劍絕卓涸縨???饜尺倛??皾歲?饆???????嫦妃霉???挿傞絕卓??絛皾歲?劌倝??涸皾歲??欽???僑?????銳霉?涸挿??欽?遤ㄤ鵶絯?妃鵳遤????臘????鯫僑?罜??侮??湡署傞僽?貓劌剣佪涸倰??剒鵛灇瑕?プ??呍?遤?椚?欽玐??封?饜尺倛??涸」?皾歲????椚???霉?涸挿??絛?傞?蜦》???霉?涸絕卓?鵥????銳霉?涸挿霪皾歲罌贊?嫦???挿涸?臘絕卓?呏研?剣?臘涸絕卓雦皾蜦》?僑??霃縨務(wù)??欽?遤??鵳遤霉?傞??椚挿??た絯?妃務(wù)ず傞倝罜割僽鷶?劌倝??僑??僑霉?涸????卓??務(wù)尵剣?霴??涸縨??????搬罜?剣??鵴?模繠涸僑?雦皾絕卓??剣??霴??姼銳?絕卓涸縨??鵳遤靈侮?饜尺倛??皾歲涸霉???霹僈GP涸饜尺倛??皾歲????????」????霚鸑鵂???呋??剒???霃雦傞暵?雮霪?僑?剣????ㄤ??剒??崵霚??皾歲僽や爍???????剒?????勻??剣??鱈?ㄤ??鱈?涸碫??」??僑勻霹僈??倰歲??皾歲?剒た??霃縨??嫦鯫霉?涸挿僑????鵳遤勉邂?滿嫦?錞垷涸?????劥????告??鯫僑???侮??湡蔄餩涸傞??????????鵮「???勵(lì)?涸??暵?僽?鸑?欰暟??涸叻?96?欰暟ざ?鸀?涸荈???欰暟ざ?鸀?欰??ㄤ???涸????僽鸑?割?邂??導(dǎo)?鳥檜??荝導(dǎo)?務(wù)務(wù)????涸獤稡?署?搬罜?銳??ざ?鸀?務(wù)嫦?姿?涸撐練?邂僽????涸????ぐ貓割ず??????鸑??鸑鵂?靈???騟?務(wù)嫦?姿?涸鸑?勻?梡?鵯???涸?韌嚋???欽邍鴪-欰??僑勻邍爙??邍鴪宐?勻?梡?研姼??????霃縨???靈侮鸑騟務(wù)??涸邍鴪?僑涸鱈?????銳涸剒??鱈??た?霚?割ず涸??宐???鱈?????鱈??罜???剒?鱈?湱??涸鱈??薉繠瘝?梡剣涸欰暟?鳴??搬????涸???雦臘?割???渹絈脅????晙媯DNA涸?鳴?劥???鸑?崵?媯????康?欽遰?割稒?瘝?雽?灇封?寵?勱臘忘駈??涸封?????烯??????助劼匬涸?玐???湱嬙???務(wù)?猰??帿??鵳?助?涸ざ?欰暟灇瑕??猰??炄霃倶?務(wù)?猰??灇瑕?涸欰暟霃雦務(wù)?瘝?去邍?剣荈?涸暵挿??倝?罜?俒??絏嵄寐??匆???猰?務(wù)???涸欰暟???蘭鳴灇瑕??霃涸ざ?欰暟?荈??鄳縨iBioFoundry??匬?帿?輑ざBT+IT欰暟絑崸+僑?絑崸涸紩ざ?欰暟?灇瑕???iBioFoundry?欽垷??霃雦?欽鲪麤?劼唒苮侮ざぐ貓??霃?ゎ呋劥??DNA??絆鄳?絈脅癃鷥???ㄤ?區(qū)唬崵???臘垷??侮?霃雦???佞?????湱??匠た劍??鸑鵂霃?涸?獴????幑??梡霃???涸?崞」??鄳縨?臘涸?紩?呏研割ず垷?涸??寵ㄤ暵挿鄳縨???欽??紩ㄤ???劼唒苮?罜??涸?佪檜??珘??ㄤ???劼唒苮乼??崞涸暵??鄳縨務(wù)劼唒苮???剣?ワ霃?湬??匹鼙???厤?霃雦務(wù)??霃??銳鸑鵂獴巊???姼??霃?靈欽涸?崞????鄳縨務(wù)絑??銳鵳遤署勞霚?邉????劌?涸霃??霃?????徾鲪?鯪渹?倰???欽ㄤ絶?????梠?涸偽蝒?寵DNA??絆鄳ㄤ絈脅癃鷥??????霃雦???銻渷?HEPA??忘駈割ず猰灇???寵剒?玐???鄳縨?欽佪檜???????鵳玐盜椚?臘臘鷅駛雵?????崨玐涸鵂玐?椚???梡?剣呋劥涸鷅駛彷彂?鄳縨涸??ㄤ暵挿??荈??偽????????ず??ず傞鵘遤鵘遤鵂玐務(wù)??傞?佖?幑?倝涸????鵘遤鵂玐務(wù)???椚涸稒?呋劥鵘遤鵂玐務(wù)??邉?署勞?霚?呏研??絕卓模?蘭臘?鶴鰳?倗?剣模?涸霃?佦??撐瘻殜?梡鵴玐ㄤ???佐?????臘瘝?DN銳????????晙媯涸的?????鵶??鯪???ぐ貓欰?導(dǎo)??禹涸??瘝?霪垷???乼?鸑??呋?匣?ぐ霃??獴??籖???紩劼唒苮?剣佪檜??珘???涸暵?臘???傞????檜涸?餏蚚???姼霪垷?務(wù)?????臘涸??紩F7劼唒苮勻模?呋劥?ぐ霃??涸鯪獴???侮ざ涸霃???獴巊??畀?紵?紩?嵐獴巊畀?荈???芘劼ㄤ丘芘劼?荈??PCR??荈??猌?劼ㄤ荈??署勞??霃?瘝?絈脅癃鷥???垷???涸?銳???????「?絈脅???蝒巊荈?嶃匣??匣?縨??????》?蝒巊?虹???霶?邍鴪?F7劼唒苮勻鵳遤呋劥?ぐ霃??涸?鷻侮ざ涸霃???獴巊??畀?荈??姻?鵂応鄳縨???獤?鳥?巊??荈???虹??盲??縨??盲?荈???庛?盲??鳥??猌?劼??叻???署勞??霃?瘝??區(qū)唬崵垷????佪巊湱蒀靶??荈??呍?晙媯?區(qū)??脅??蚃???PCR????區(qū)唬崵霃?ㄤ??署勞???叼?忘駈餘唬????????邍鴪?區(qū)?跗涯?絈脅去靧?暟?區(qū)瘝?貓唬崵?寵?割ず??鶣??垷??區(qū)唬崵垷??欽?佞?霃雦??傞?佐??鳥?區(qū)唬崵呋????署勞?霚?涸?傞幑邉?霃?涸傈?絶??????????寵ㄤ???罌贊?區(qū)唬崵垷?鷥??欽???劼唒苮模?ぐ霃??垷??涸??呋劥?鷐?呋劥?垷?欽?蝒貓ㄤざ?欰暟???涸??ㄤ》欽???餛??呏0.5ml呋?盜涸-80°C荈??欰暟呋劥??霃?labtech????涸?瀦?ㄤ?渷劼?呋劥?垷??絈脅癃鷥????欽????紩F7iBioFoundry?鵘遤呩?iBioFoundry湡?????梡?肅勍蝒??嫢絈脅?衛(wèi)虊蔠?勍蝒??問勍蝒瘝?貓???欰暟??絈脅涸?荈?匬????勍蝒?玐蝒涸??匬????絏?湡荈??倰呩涸??鵂玐???iBioFoundry????涸???鸑鵂?涸?ぢ鵳??癃鷥呩????崨玐涸蘭臘?鷥?ㄤ??鸑?鵳遤?區(qū)???鍒?9?????妃僽PCR導(dǎo)?呍?晙媯?區(qū)?DNA絆鄳?餘磜鯪??蝒巊嶃????????蝒釁????蝒吳?????鷓?荈??乼?????倰歲???靈侮?煂棟紱?歲去剏?臉?佐?PCR?欽荈??呍?晙媯?區(qū)?唬?PCR?欽蚃???PCR??蝒吳匬?涸姻爍?鵳遤?霆??邍23.?玐蝒??匬???崨玐 餴俱勻彂SyntheticBiologyJournal?邍24.?????姿?ㄤ?劼玐? 餴俱勻彂SyntheticBiologyJournal25.??崨玐荈??玐?綘?爙?餴俱勻彂SyntheticBiologyJournal涸絕匬??欽?鸑?癃鷥?媯?????剣暵???崞??珘???鷥??涸??忘駈ぐ貓灇瑕??欽?寵?????務(wù)??瑲」?涸乼??寵鸑??銳??鸑?涸???ㄤ僑研?區(qū)?媯湱絕ざ???灇瑕佪檜?????叻唬崵???癃倰歲絕ざ?佪巊湱蒀靶?區(qū)??撐癃倰歲??iBioFoundry?模???ぢ鵳?涸??呩?鵳遤霹僈????欽僒?PCR?????挿鵳遤?劼瑲」瘝倰歲匬?湡叻??」俒?鯪??肅勍蝒た?欽獴巊??畀涸???鷥???》蝒釁鵳遤?呏研OD?唬崵僑研??蝒?欰?????劼霶?霶?邍OD?????屲邍鴪涸?跗涯?匣絑猌?》?幡???磦?巊??卓僽脅?跗涯猌?佐?蝒?た??鄬鍒巊絑?虹?肫灶烱絈脅鄬鍒巊猌?た》?幡蜦?磦?巊??欽?叻?唬崵?崞蒀靶撐癃?jiǎng)zた呏研唬崵僑研癃鷥??蝒吳??欽?た絯崵??區(qū)?鵳???鯫涸?ぢ鵳?????欽務(wù)?鯡?務(wù)涸」????鶴鰳?倗??梠?僑研?》??????臘??梡???崨玐涸蘭臘?????劥呩?涸??崨玐務(wù)挿??鸑鵂??傞唬崵僑研涸?區(qū)罜?梡??康??崨玐涸蘭臘鷥???僽OD?僑研鷥?霶?傞劼呏研?叻唬崵僑研癃鷥呋劥鵳?撐癃呏研巊湱蒀靶唬崵僑研癃鷥蝒巊呋劥?殆?欽??邍?邍26.?涸?ぢ鵳??癃鷥??崨玐 餴俱勻彂SyntheticBiologyJournal

inry?霃??挿絑?荈?iBioFoundry??欽僽?佞??務(wù)??涸?エ??帿?匣???匣瘝ぐ碫署勞?iBioFoundry鄳縨??剣??荈??署勞??霃?????絆鄳垷??絈脅癃鷥???垷??ㄤ?區(qū)唬崵垷????????遤??????遤臘????iBioFoundry涸鵘遤佪檜?劥禹絡(luò)涸??鯡?????涸??鵳玐盜椚?臘務(wù)ぐ鵳玐涸剒?傞?ㄤ霃?餴彂靈?鴪????ず傞鵘遤涸湡叻??姼?炄?iBioFoundry鵮?雽???鵳遤務(wù)涸??傞?挿幑?倝?撐???寵??傞?ㄤ餴彂??ず傞??????涸鵘遤佪檜???崨玐叻???霃荈????倰呩涸??鵂玐務(wù)?銳????????綘?湱?涸荈??玐??iBioFoundry務(wù)??崨玐涸叻??????崨玐務(wù)ぐ????涸荈??玐?綘?ㄤ崵霚錞薴??剒絊??侮???崨玐涸叻?乼?俒咓?勱勻展?欰暟?涸蘭臘??劅ざ?欰暟?涸呍?鶴鰳僽??玐?椚?雦皾霃雦???叻???ㄤ?韌?瘝?欰暟?鳴?涸崨宐絁?助湱絕ざ?梡??搬罜?剣??薴?涸欰暟?????鸑鵂????倶涸霃雦-匬崵霚-???梠勻鍒??銳?厤貓封?鵂玐ㄤ???剣??欰暟?鳴??????欰暟涸?姿?封???欰暟絆?玐告????歊??罏涸肅麤務(wù)????「??ず椚嗃暟ㄤ?20??籖婤?妃?緵務(wù)?ざ?欰暟??割模??饆?雦皾霃雦ㄤ叻????欨蕘剒?ざ?欰暟???呩??劌?????罈?涸霚??靈霚倰歲罜割僽??歏?涸?玐?椚雿??紱???雦皾?崵?務(wù)?涸椚鍒倰????ざ?欰暟?涸勱勻封?匬??ざ?欰暟?涸劥餘僽?倝?韌欰ㄐ鵂玐欨蕘??欰ㄐ劥魧?臘僽歷??ざ?欰暟?》綖?鸑鵂?鳴撐練霃?涸?玐?椚?欽?欰暟?涸?錞??鯪罜鸑鵂?欽欰暟鵳??鳴涸???割ず鶴鰳勻鍒??湡叻??涸???20186勻荈?椕15???椕欰暟?鳴??靂???去邍雩?欰暟?鳴???兜麑??滿湱ず涸????傱餥涸?炄霃倶涸絶?餩欽ㄤ鵘蠒?プ涸餩欽緃?鸑欽涸霃倶鯡熾?叻?ㄤ濼霋?勉??劼?欰暟??勞俱??騗???悶?欽??荈?緃??鍒瘝??姼ざ?欰暟?荈????涸勱勻封?饞???絆?翫湅灇?鸑欽涸乼?禹絡(luò)?荈???靂?叻???遤?侮????勞俱涸?????劥鼙??撐灇??絗荈??霃倶???畮剪?的?欽?纈?暵?僽務(wù)???欽????靈????署勞??休?ず?休??瘝?ざ?欰暟?荈????霃倶涸?霃?傉饑??鳥封?涸鲪麤?????涸?毫?臘彂ㄤ梠?瘝???????倝鍒?倰呩?碫涸欰?ㄤ欰崞倰??絕ざ?俒????劼???ㄤ荈????倰?錜?勱勻封?饞??湡涸灇瑕倰歲匬??????欰暟????臘?卓?勵(lì)鵮??霽??????皍?涸絁?垷?勻霹?暵???崵絕卓涸?銳??蕯鱈?暵?湱匹杝用絁?垷???剣??涸?鍒???搬罜??撐練垷??牟絑緸絞暵?ㄤ湡叻?????絁??禹垷?涸?鍒???」涸匧??〥????欰暟緸絞???靈?緸騟??????靈??巭佪??銳???卓?倗鍒?靈??禹??涸幊巭??滿??絆倰?涸?撐??僑研割倗??,?卓?禹垷??劼???????餿勻餿?涸?岤湱??卓?禹垷?????封?悶??〥?跗涯餘瘝???臘?垷?僽劼???灇瑕務(wù)餿勻餿「妅鵓涸搬罜銳蜦》剣叻皊涸呋劥鸑??銳??鰍?涸去???呋蜦?剣叻皊涸呋劥僑研??餴彂嵠餩?癃鷥?偽?臘跗涯????瘝??呋劥涸嚋檜??ず傞?呋鸑鵂剣?涸呋劥僑研匬?癃鷥垷?僽劼????助?勱勻?銳?挿鍒?涸?????????涸荈???倰臘溫姻?梡?????僽???勱鍒?涸ざ?欰暟???ㄤ???銳?涸????欰暟?玐?欰暟蝒吳?鳥檜佐檜禹絡(luò)???僽???銳?????休??遤?涸鵂玐務(wù)?烯僽鴪?絑崸?遤涸欰暟欰????涸TRY宐???絡(luò)倰歲嶍???蝒吳暵霚確?絆ざ鵂玐?-?-?倰歲告??????鸀?ㄤ??務(wù)鯪獴佪卓??僽剒呍?涸嶍??????絆???絆?涸劼?椚鍒偽歲?爍?????崵??欰暟??絛???絆???涸遤????梠?????暟???告??湡?緃?霃雦忘駈暵?錞薴涸?欰暟纈釁涸濼霋??霃雦?珘?鳥檜??宐務(wù)??熧涸?欰暟纈釁??遤僤/?僤梠?涸導(dǎo)??鲪麤ㄤ鲪麤??涸暟崨?劥???傱?姼?瑟?jiǎng)?wù)涸ㄤ霃???剣???鶣嫦?????銳?湱?涸SDLs霃縨割ず涸劼?霃??嫦?SDL?劥????臘湬?湱??薴??欽撐練?SDLs?傱罜鰍皍??SDLs??臘湱?鰍??SDLs?猰???剒?銳涸???雽勻荈??臘?荈?匬?猰?濼霋涸臘?猰?濼霋?涸臘?鍒?ㄤ?崵?灇瑕禹絡(luò)涸遤?涸???嚋???禹???霃?SDLs臘?呏研?銳???涸濼霋ㄤ??餴彂務(wù)?》?鵳遤??勻佖鵳鵯?濼霋?鵯貓佖鵳??梡??劼?涸劌帿?椚鍒ㄤ劌??涸?崵臘?????霃?SDLs??僑??欰涸?????獤稡涸濼霋??欽??滿???區(qū)涸欰暟禹絡(luò)涸?鍒??罜封欰怵」???禹絡(luò)?欰ㄐ?爢?欨蕘??涸貞?撐????????歲涸餴??僑??欰涸剒??欽?僽??爍?????湱匹?禹罜??涸???靂??傉?欰?駈?涸??僑研勻霋?鵯??翫??爍????翫僽?卓?禹???卓?禹?僈僑??欰涸?欽?」?霃雦???霆??臘?鍒?ㄤ???崵鵯??卓佪?涸劼?椚雿??傉鵯?椚雿鄄吥?僑??欰涸錭蒀?鯪」?霃雦??匬?忘駈?劍錞呔涸倝欰暟禹絡(luò)鷟ぢ霃雦?鵯貓匬?猰?濼霋涸瘻殜?覆?幊ざ倰歲??紱礨涸SDLs??碫??絡(luò)???ㄤ梡剣俒柄湱絕ざ??邍27.??霃?SDLs????SDLsㄤ?碫???緸絞務(wù)???SDLs臘??梡剣涸俒柄務(wù)蜦》???臘湱????猰?濼霋涸朐?》?鵳?鵮?臘????SDLs鵳遤鸑佪?夠??確程涸猰?湱瑟?dú)S?剣?臘涸??縨???僑鷥?絆?涸?韌瑟???SDL1?岤?絆???SDL2?岤〥?絆????湡?涸?助?????荈???剣??涸臘??姼SDLs?霪臘??欰僈爍涸?碫??絡(luò)???務(wù)黽???》??欰涸僑研霪緸絞鵘遤涸剒絊絕卓?僽?灇瑕涸禹絡(luò)鵳遤僑??欰鵯?僑??欰?臘剒?僽?禹絡(luò)????鵶?涸皍?ㄤ???鶣滿蜦?倝??涸???封??劌撐練涸劼?垷?ㄤ??灇瑕禹絡(luò)涸???崵垷??侮?絈脅垷??剣?爍涸?崵???欽鵯?僑??欰勻霄?鵯?幊ざ緸絞欰?涸猰?濼霋???荈?涸?靂? 餴俱勻彂ScienceDirect模Ls?ㄤ爢?涸???鵳姿?騄覆???荈??助???蘭臘皾歲?僑研盜椚???銳涸爢??烯涸???巊??椚??畀?欽?ざ?欰暟?荈??涸鵂玐姻覿?封?SDLs??涸鵂玐?僽ざ?欰暟???鵂玐涸??????涸??欰暟?鵂玐?絈脅鯪??蝒釁?鷥??匣??ㄤ欰?