Thiazolyl-Blue-MTT-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEThiazolylBlueCat.No.:HY-15924CASNo.:298-93-1Synonyms:MTT;ThiazolylBlueTetrazoliumbromide;Methylthiazolyldiphenyl-tetrazoliumbromide分?式:C??H??BrN?S分?量:414.32作?靶點(diǎn):Others作?通路:Others儲(chǔ)存?式:4°C,sealedstorage,awayfrommoistureandlight*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoistureandlight)溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:25mg/mL(60.34mM;ultrasonicandwarmingandheatto60°C)H2O:0.88mg/mL(2.12mM;Needultrasonicandwarming)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM2.4136mL12.0680mL24.1359mL5mM0.4827mL2.4136mL4.8272mL10mM0.2414mL1.2068mL2.4136mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoistureandlight)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的?式助溶)1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE1.請(qǐng)依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.08mg/mL(5.02mM);Clearsolution2.請(qǐng)依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.08mg/mL(5.02mM);Clearsolution3.請(qǐng)依序添加每種溶劑：PBSSolubility:6.67mg/mL(16.10mM);Clearsolution;NeedultrasonicBIOLOGICALACTIVITY?物活性ThiazolylBlue(MTT)?種?泛?于測(cè)量細(xì)胞增殖的??劑。ThiazolylBlue(MTT)在活細(xì)胞中從?還原為紫?甲。體外研究ThiazolylBlue(MTT)isusedinconjunctionwithrhodamineBtomeasuremitochondrialmembranepotential.ThiazolylBlue-formazanisgeneratedinmitochondriaactsasafluorescencequencherforrhodaminethatdistributesacrossmembranesofviablecellsaccordingtomembranepotential.IntheabsenceofmPMS,cellularreductionofThiazolylBlueisstrong.ThiazolylBlueismembrane-impermeablewhenincorporatedintolargeunilamellarliposomes,andthereforeitistakenupbycellsviaendocytosis[1].戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?CellMolImmunol.2021Mar;18(3):613-620.?NatCommun.2022Nov10;13(1):6796.?BioactMater.2020Mar30;5(2):398-409.?JHazardMater.2020Apr5;387:121686.?MaterTodayBio.19July2022,100369.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].BerridgeMV,etal.Tetrazoliumdyesastoolsincellbiology:newinsightsintotheircellularreduction.BiotechnolAnnuRev.2005;11:127-52.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.For