香蕓火絨草化學成分的研究

畢業(yè)論文（設(shè)計）香蕓火絨草化學成分的研究目錄TOC\o"1-2"\h\u21485摘要 摘要香蕓火絨草（Leontopodiumhaplophylloides)屬菊科火絨草屬植物。我國集中分布于產(chǎn)四川西部（道孚）和北部（松潘）、青海東部（西寧、大通等）、甘肅西南部（臨潭）等地，生長于高山及亞高山地區(qū)，是民間常用的藥用植物。具有清熱、涼血、清炎、利尿等功效?；鸾q草具有確切的藥理活性和臨床應(yīng)用，值得我們進一步研究。本文對其化學成分進行研究。采用硅膠柱層析、薄層層析技術(shù)對其成分進行了分離、純化,并采用核磁共振技術(shù)鑒定了其中兩種化合物的結(jié)構(gòu)，分別為：香豆類化合物obliquin、豆甾醇（stigmasterol）。關(guān)鍵詞香蕓火絨草化學成分柱層析核磁波譜分析AbstractXiangYunedelweiss(Leontopodiumhaplophylloides)belongtotheAsteraceaeedelweissspecies.TheyareconcentratedintheproductionofwesternSichuan(Dawu)andNorth(Songpan),easternQinghai(Xining,Chase,etc.),GansuProvinceinthesouthwest(Engagingin)otherplaces,growthinthealpineandsub-alpineregions,iscommonfolkmedicinalplants.Edelweisshascuredheat,cooling,anti-inflammatoryanddiureticeffects.Leontopodiumhaplophylloideshasadefinitepharmacologicalactivityandclinicalapplication,itisworthfurtherstudy.Inthispaper,itschemicalcompositionwasstudied.Theuseofsilicagelcolumnchromatography,thinlayerchromatographyfromedelweissextract,itscomponentswereisolatedandpurified.WeusetheNMRspectroscopytoidentifythestructureinwhichtwocompounds:fragrantbeanscompoundobliquin,stigmasterol.KeywordsLeontopodiumhaplophylloides,chemicalconstituents，chromatography，NMRspectroscopy一、前言（一）香蕓火絨草簡介香蕓火絨草（Leontopodiumhaplophylloides），屬植物界，雙子葉植物綱，被子植物門，火絨草屬的桔梗目菊科植物。1.香蕓火絨草的形態(tài)描述多年生草本。根狀莖短小，簇狀叢生。莖直立，高15-30厘米，纖細，堅挺，下部木質(zhì)，不分枝，僅砍斷的莖有分枝，被蛛絲狀毛，上部常有腺毛，全部有密生而等距的葉；節(jié)間長0.5-12厘米。葉稍直立或開展，狹披針形或線狀披針形，基部漸狹，無柄，邊緣稍反卷或平，頂端長尖或尖，有細小尖頭，草質(zhì)，兩面被灰色或青色短茸毛。2.產(chǎn)地分布于\o"中國大陸"中國大陸的\o"青海"青海、\o"四川"四川、\o"甘肅"甘肅等地，生長于海拔2,600米至4,000米的地區(qū)，一般生長在石礫地、灌叢、高山草地和針葉林處緣ADDINEN.CITE<EndNote><CiteExcludeAuth="1"><Year>1965</Year><RecNum>48</RecNum><DisplayText><styleface="superscript">[1]</style></DisplayText><record><rec-number>48</rec-number><foreign-keys><keyapp="EN"db-id="e2ep9dwvowt0f4eas51vzp24fwrrtpsar5fd">48</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors></contributors><titles><title><styleface="normal"font="default"size="100%">Leontopodiummicrocephalum(Hand</style><styleface="normal"font="default"charset="134"size="100%">．-Mazz.)Ling</style></title><secondary-title><styleface="normal"font="default"charset="134"size="100%">植物分類學報</style></secondary-title></titles><periodical><full-title>植物分類學報</full-title></periodical><pages>173</pages><volume>10</volume><number>05</number><dates><year>1965</year></dates><urls></urls></record></Cite></EndNote>[\o",1965#48"1]。（二）火絨草屬的藥理活性及臨床作用1.藥理活性（1）抗炎作用:黃利權(quán)對火絨草3種水溶性組分對小鼠耳廓腫脹的抑制作用表明火絨草具有較強的抗炎活性ADDINEN.CITE<EndNote><Cite><Author>黃利權(quán)</Author><Year>2004</Year><RecNum>72</RecNum><DisplayText><styleface="superscript">[2]</style></DisplayText><record><rec-number>72</rec-number><foreign-keys><keyapp="EN"db-id="e2ep9dwvowt0f4eas51vzp24fwrrtpsar5fd">72</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author><styleface="normal"font="default"charset="134"size="100%">黃利權(quán)</style></author><author><styleface="normal"font="default"charset="134"size="100%">伍義行</style></author></authors></contributors><titles><title><styleface="normal"font="default"charset="134"size="100%">火絨草的抗炎活性研究——對二甲苯所致小鼠耳廓腫脹及毛細血管通透性的影響</style></title><secondary-title><styleface="normal"font="default"charset="134"size="100%">中獸醫(yī)學雜志</style></secondary-title></titles><periodical><full-title>中獸醫(yī)學雜志</full-title></periodical><pages>10-12</pages><volume>117</volume><number>2</number><dates><year>2004</year></dates><urls></urls></record></Cite></EndNote>[\o"黃利權(quán),2004#72"2]。姜鴻等測定了火絨草屬抗急性炎癥的藥效物質(zhì)基礎(chǔ)ADDINEN.CITE<EndNote><Cite><Author>鴻</Author><Year>2010</Year><RecNum>52</RecNum><DisplayText><styleface="superscript">[3]</style></DisplayText><record><rec-number>52</rec-number><foreign-keys><keyapp="EN"db-id="e2ep9dwvowt0f4eas51vzp24fwrrtpsar5fd">52</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author><styleface="normal"font="default"charset="134"size="100%">姜鴻</style></author><author><styleface="normal"font="default"charset="134"size="100%">侯政</style></author><author><styleface="normal"font="default"charset="134"size="100%">邸子真</style></author><author><styleface="normal"font="default"charset="134"size="100%">王光函</style></author><author><styleface="normal"font="default"charset="134"size="100%">尤獻民</style></author></authors></contributors><titles><title><styleface="normal"font="default"charset="134"size="100%">火絨草抗急性炎癥藥效物質(zhì)基礎(chǔ)初步研究</style></title><secondary-title><styleface="normal"font="default"charset="134"size="100%">中成藥ChineseTraditionalPatentMedicine</style></secondary-title></titles><periodical><full-title>中成藥ChineseTraditionalPatentMedicine</full-title></periodical><pages>1048-1050</pages><volume>32</volume><number>6</number><dates><year>2010</year></dates><urls></urls></record></Cite></EndNote>[\o"鴻,2010#52"3]。（2）降血糖作用:根據(jù)焦淑萍等對火絨草的降血糖作用的研究,用火絨草的水萃取液對小鼠灌胃，發(fā)現(xiàn)其對四氧嘧啶引起的糖尿病有顯著的預(yù)防作用,表明火絨草可能改善受損的β細胞的功能，并削弱四氧嘧啶對胰島β細胞的損傷，可起到預(yù)防和治療糖尿病的藥用價值A(chǔ)DDINEN.CITE<EndNote><Cite><Author>焦淑萍</Author><Year>1997</Year><RecNum>50</RecNum><DisplayText><styleface="superscript">[4]</style></DisplayText><record><rec-number>50</rec-number><foreign-keys><keyapp="EN"db-id="e2ep9dwvowt0f4eas51vzp24fwrrtpsar5fd">50</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author><styleface="normal"font="default"charset="134"size="100%">焦淑萍</style></author><author><styleface="normal"font="default"charset="134"size="100%">姜虹</style></author><author><styleface="normal"font="default"charset="134"size="100%">任慶林</style></author></authors></contributors><titles><title><styleface="normal"font="default"charset="134"size="100%">火絨草對四氧嘧啶糖尿病小鼠高血糖的降低作用</style></title><secondary-title><styleface="normal"font="default"charset="134"size="100%">北華大學學報</style></secondary-title></titles><periodical><full-title>北華大學學報</full-title></periodical><pages><styleface="normal"font="default"size="100%">10</style><styleface="normal"font="default"charset="134"size="100%">－</style><styleface="normal"font="default"size="100%">11</style></pages><volume>17</volume><number>2</number><dates><year>1997</year></dates><urls></urls></record></Cite></EndNote>[\o"焦淑萍,1997#50"4]。方玉春等設(shè)計了藥料先用pH=3的酸水提取,繼而用pH=9的堿水提取的半仿生提取法，可以更加明顯的發(fā)揮火絨草的降血糖作用ADDINEN.CITE<EndNote><Cite><Author>方玉春*</Author><Year>2007</Year><RecNum>66</RecNum><DisplayText><styleface="superscript">[5]</style></DisplayText><record><rec-number>66</rec-number><foreign-keys><keyapp="EN"db-id="e2ep9dwvowt0f4eas51vzp24fwrrtpsar5fd">66</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author><styleface="normal"font="default"charset="134"size="100%">方玉春</style></author><author><styleface="normal"font="default"charset="134"size="100%">辛現(xiàn)良</style></author><author><styleface="normal"font="default"charset="134"size="100%">朱偉明</style></author><author><styleface="normal"font="default"charset="134"size="100%">顧謙群</style></author></authors></contributors><titles><title><styleface="normal"font="default"charset="134"size="100%">老頭草半仿生提取物的降血糖作用研究</style></title><secondary-title><styleface="normal"font="default"charset="134"size="100%">天然產(chǎn)物研究與開發(fā)NatProdResDev</style></secondary-title></titles><periodical><full-title>天然產(chǎn)物研究與開發(fā)NatProdResDev</full-title></periodical><pages><styleface="normal"font="default"size="100%">508</style><styleface="normal"font="default"charset="134"size="100%">-</style><styleface="normal"font="default"size="100%">510</style></pages><volume>19</volume><number>5</number><dates><year>2007</year></dates><urls></urls></record></Cite></EndNote>[\o"方玉春,2007#66"5]。（3）抗菌作用:伍義行等對火絨草提取物進行了體外抑菌實驗，實驗證明了火絨草對金色葡萄球菌具有較好的抑制作用ADDINEN.CITE<EndNote><Cite><Author>伍義行</Author><Year>2000</Year><RecNum>51</RecNum><DisplayText><styleface="superscript">[6]</style></DisplayText><record><rec-number>51</rec-number><foreign-keys><keyapp="EN"db-id="e2ep9dwvowt0f4eas51vzp24fwrrtpsar5fd">51</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author><styleface="normal"font="default"charset="134"size="100%">伍義行</style></author><author><styleface="normal"font="default"charset="134"size="100%">王建國</style></author><author><styleface="normal"font="default"charset="134"size="100%">郭福存</style></author></authors></contributors><titles><title><styleface="normal"font="default"charset="134"size="100%">火絨草抑菌作用試驗研究</style></title><secondary-title><styleface="normal"font="default"charset="134"size="100%">時珍國醫(yī)國藥</style></secondary-title></titles><periodical><full-title>時珍國醫(yī)國藥</full-title></periodical><pages><styleface="normal"font="default"size="100%">289</style><styleface="normal"font="default"charset="134"size="100%">－290</style></pages><volume>11</volume><number>4</number><dates><year>2000</year></dates><urls></urls></record></Cite></EndNote>[\o"伍義行,2000#51"6]。喬君霞等對新疆短星火絨草不同極性提取物的抑菌作用的研究表明其具有高效廣譜的抑菌特點ADDINEN.CITE<EndNote><Cite><Author>喬君霞</Author><Year>2013</Year><RecNum>73</RecNum><DisplayText><styleface="superscript">[7]</style></DisplayText><record><rec-number>73</rec-number><foreign-keys><keyapp="EN"db-id="e2ep9dwvowt0f4eas51vzp24fwrrtpsar5fd">73</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author><styleface="normal"font="default"charset="134"size="100%">喬君霞</style></author><author><styleface="normal"font="default"charset="134"size="100%">索菲婭</style></author><author><styleface="normal"font="default"charset="134"size="100%">黃羅冬</style></author><author><styleface="normal"font="default"charset="134"size="100%">馬贊</style></author><author><styleface="normal"font="default"charset="134"size="100%">鐘玨</style></author></authors></contributors><titles><title><styleface="normal"font="default"charset="134"size="100%">新疆短星火絨草不同極性提取物抑茵作用研究</style></title><secondary-title><styleface="normal"font="default"charset="134"size="100%">云南農(nóng)業(yè)大學學報JournalofYunnanAgriculturalUniversity</style></secondary-title></titles><periodical><full-title>云南農(nóng)業(yè)大學學報JournalofYunnanAgriculturalUniversity</full-title></periodical><pages>83-87</pages><volume>28</volume><number>1</number><dates><year>2013</year></dates><urls></urls></record></Cite></EndNote>[\o"喬君霞,2013#73"7]。（4）對多核白細胞移行作用的影響:黃利權(quán)等發(fā)現(xiàn)火絨草3種提取組分對奶牛乳腺多形核白細胞的體外吞噬活性具有良好的調(diào)節(jié)作用ADDINEN.CITE<EndNote><Cite><Author>黃利權(quán)</Author><Year>2005</Year><RecNum>55</RecNum><DisplayText><styleface="superscript">[8]</style></DisplayText><record><rec-number>55</rec-number><foreign-keys><keyapp="EN"db-id="e2ep9dwvowt0f4eas51vzp24fwrrtpsar5fd">55</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author><styleface="normal"font="default"charset="134"size="100%">黃利權(quán)</style></author><author><styleface="normal"font="default"charset="134"size="100%">伍義行</style></author></authors></contributors><titles><title><styleface="normal"font="default"charset="134"size="100%">火絨草水提取液對牛乳中多形核白細胞體外殺菌功能的影響</style></title><secondary-title><styleface="normal"font="default"charset="134"size="100%">浙江農(nóng)業(yè)學報ActaAgriculturaeZhejiangensis</style></secondary-title></titles><periodical><full-title>浙江農(nóng)業(yè)學報ActaAgriculturaeZhejiangensis</full-title></periodical><pages>55-59</pages><volume>17</volume><number>2</number><dates><year>2005</year></dates><urls></urls></record></Cite></EndNote>[\o"黃利權(quán),2005#55"8]。李龍云等通過火絨草95%乙醇、正丁醇濃縮提取物對動物變態(tài)反應(yīng)性炎癥的影響發(fā)現(xiàn)該提取物能夠明顯抑制局部組織水腫和多核白細胞的移行ADDINEN.CITE<EndNote><Cite><Author>李龍云</Author><Year>1994</Year><RecNum>53</RecNum><DisplayText><styleface="superscript">[9]</style></DisplayText><record><rec-number>53</rec-number><foreign-keys><keyapp="EN"db-id="e2ep9dwvowt0f4eas51vzp24fwrrtpsar5fd">53</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author><styleface="normal"font="default"charset="134"size="100%">李龍云</style></author><author><styleface="normal"font="default"charset="134"size="100%">葉金梅</style></author><author><styleface="normal"font="default"charset="134"size="100%">尹航</style></author><author><styleface="normal"font="default"charset="134"size="100%">朱宇明</style></author><author><styleface="normal"font="default"charset="134"size="100%">田建明</style></author><author><styleface="normal"font="default"charset="134"size="100%">高峰</style></author></authors></contributors><titles><title><styleface="normal"font="default"charset="134"size="100%">火絨草對動物變態(tài)反應(yīng)性炎癥的影響</style></title><secondary-title><styleface="normal"font="default"charset="134"size="100%">中國中藥雜志</style></secondary-title></titles><periodical><full-title>中國中藥雜志</full-title></periodical><pages>174-175</pages><volume>19</volume><number>3</number><dates><year><styleface="normal"font="default"charset="134"size="100%">1994</style></year></dates><urls></urls></record></Cite></EndNote>[\o"李龍云,1994#53"9]。Schwaiger通過體外試驗發(fā)現(xiàn)火絨草提取物可抑制白三烯的生物合成ADDINEN.CITE<EndNote><Cite><Author>STEFAN</Author><Year>2004</Year><RecNum>56</RecNum><DisplayText><styleface="superscript">[10]</style></DisplayText><record><rec-number>56</rec-number><foreign-keys><keyapp="EN"db-id="e2ep9dwvowt0f4eas51vzp24fwrrtpsar5fd">56</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author>SCHWAIGERSTEFAN</author><author><styleface="normal"font="default"charset="134"size="100%">ADAMSMICHAEL</style></author><author><styleface="normal"font="default"charset="134"size="100%">SEGERCHRISTOPH</style></author></authors></contributors><titles><title>NewconstituentsofLeontopodiumalpinumandtheirinvitroleukotrienebiosynthesisinhibitoryactivity</title><secondary-title>PlantaMedica</secondary-title></titles><periodical><full-title>PlantaMedica</full-title></periodical><pages><styleface="normal"font="default"size="100%">978</style><styleface="normal"font="default"charset="134"size="100%">－985</style></pages><volume>70</volume><number>10</number><dates><year>2004</year></dates><urls></urls></record></Cite></EndNote>[\o"STEFAN,2004#56"10]。（5）抗氧化作用:展銳等發(fā)現(xiàn)火絨草水提物和醇提物（多酚類物質(zhì)）具有很強的還原性，能夠清除自由基和亞硝酸鈉，阻斷亞硝胺的合成ADDINEN.CITE<EndNote><Cite><Author>展銳</Author><Year>2010</Year><RecNum>54</RecNum><DisplayText><styleface="superscript">[11]</style></DisplayText><record><rec-number>54</rec-number><foreign-keys><keyapp="EN"db-id="e2ep9dwvowt0f4eas51vzp24fwrrtpsar5fd">54</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author><styleface="normal"font="default"charset="134"size="100%">展銳</style></author><author><styleface="normal"font="default"charset="134"size="100%">庫爾班</style></author><author><styleface="normal"font="default"charset="134"size="100%">茍萍</style></author><author><styleface="normal"font="default"charset="134"size="100%">索菲婭</style></author></authors></contributors><titles><title><styleface="normal"font="default"charset="134"size="100%">火絨草提取物抗氧化活性的研究</style></title><secondary-title><styleface="normal"font="default"charset="134"size="100%">食品科學</style></secondary-title></titles><periodical><full-title>食品科學</full-title></periodical><pages>153</pages><volume>31</volume><number>03</number><dates><year>2010</year></dates><urls></urls></record></Cite></EndNote>[\o"展銳,2010#54"11]。吳楠貞等通過對醇提物乙酸乙酯部分進行硅膠柱層析得到了豐度較高的對羥基苯乙酮并證實其抗氧化功效ADDINEN.CITE<EndNote><Cite><Author>吳楠貞</Author><Year>2013</Year><RecNum>63</RecNum><DisplayText><styleface="superscript">[12]</style></DisplayText><record><rec-number>63</rec-number><foreign-keys><keyapp="EN"db-id="e2ep9dwvowt0f4eas51vzp24fwrrtpsar5fd">63</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author><styleface="normal"font="default"charset="134"size="100%">吳楠貞</style></author><author><styleface="normal"font="default"charset="134"size="100%">展銳</style></author><author><styleface="normal"font="default"charset="134"size="100%">茍萍*</style></author></authors></contributors><titles><title><styleface="normal"font="default"charset="134"size="100%">火絨草抗氧化活性成分及其結(jié)構(gòu)分析</style></title><secondary-title><styleface="normal"font="default"charset="134"size="100%">天然產(chǎn)物研究與開發(fā)NatProdResDev</style></secondary-title></titles><periodical><full-title>天然產(chǎn)物研究與開發(fā)NatProdResDev</full-title></periodical><pages>296-301</pages><volume>25</volume><number>2</number><dates><year>2013</year></dates><urls></urls></record></Cite></EndNote>[\o"吳楠貞,2013#63"12]。（6）增強免疫力原子吸收分光光度法測定火絨草中的Ca，Zn，F(xiàn)e，Cu，K，Na，Co，Ni，Mn，Cr等10種微量元素的含量表明其中含有豐富的人體微量元素ADDINEN.CITE<EndNote><Cite><Author>阿布來提·阿布都熱西提</Author><Year>2005</Year><RecNum>62</RecNum><DisplayText><styleface="superscript">[13]</style></DisplayText><record><rec-number>62</rec-number><foreign-keys><keyapp="EN"db-id="e2ep9dwvowt0f4eas51vzp24fwrrtpsar5fd">62</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author><styleface="normal"font="default"charset="134"size="100%">阿布來提</style><styleface="normal"font="Arial"size="100%">·</style><styleface="normal"font="default"charset="134"size="100%">阿布都熱西提</style></author><author><styleface="normal"font="default"charset="134"size="100%">買買提吐爾遜</style></author></authors></contributors><titles><title><styleface="normal"font="default"charset="134"size="100%">火絨草中微量元素含量的分析</style></title><secondary-title><styleface="normal"font="default"charset="134"size="100%">微量元素與健康研究StudiesofTraceElementsandHealth</style></secondary-title></titles><periodical><full-title>微量元素與健康研究StudiesofTraceElementsandHealth</full-title></periodical><pages><styleface="normal"font="default"size="100%">24</style><styleface="normal"font="default"charset="134"size="100%">-25</style></pages><volume>22</volume><number>1</number><dates><year>2005</year></dates><urls></urls></record></Cite></EndNote>[\o"阿布來提·阿布都熱西提,2005#62"13]，可增強機體免疫力。2.臨床作用在臨床上，民間用藥表明，藏醫(yī)用火絨草包括戟葉火絨草、長葉火絨草、矮火絨草可治療瘟病，還可解石毒，治肉瘤等ADDINEN.CITE<EndNote><Cite><Author>甘青梅</Author><Year>1997</Year><RecNum>74</RecNum><DisplayText><styleface="superscript">[14]</style></DisplayText><record><rec-number>74</rec-number><foreign-keys><keyapp="EN"db-id="e2ep9dwvowt0f4eas51vzp24fwrrtpsar5fd">74</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author><styleface="normal"font="default"charset="134"size="100%">甘青梅</style></author></authors></contributors><titles><title><styleface="normal"font="default"charset="134"size="100%">藏藥“扎托巴”的粉末鑒別</style></title><secondary-title><styleface="normal"font="default"charset="134"size="100%">中國民族民間醫(yī)藥雜志</style></secondary-title></titles><periodical><full-title>中國民族民間醫(yī)藥雜志</full-title></periodical><pages><styleface="normal"font="default"size="100%">35</style><styleface="normal"font="default"charset="134"size="100%">-37</style></pages><volume>27</volume><number>2</number><dates><year>1997</year></dates><urls></urls></record></Cite></EndNote>[\o"甘青梅,1997#74"14];蒙醫(yī)用火絨草可治療支氣管肺炎，肺熱、腹瀉、肝炎等；火絨草與黃芪、甘草搭配,可治療胃潰瘍。火絨草是東北民間治療蛋白尿、血尿等腎臟疾病的常用藥之一ADDINEN.CITE<EndNote><Cite><Author>武彥文</Author><Year>2005</Year><RecNum>59</RecNum><DisplayText><styleface="superscript">[15]</style></DisplayText><record><rec-number>59</rec-number><foreign-keys><keyapp="EN"db-id="e2ep9dwvowt0f4eas51vzp24fwrrtpsar5fd">59</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author><styleface="normal"font="default"charset="134"size="100%">武彥文</style></author><author><styleface="normal"font="default"charset="134"size="100%">高文遠</style></author><author><styleface="normal"font="default"charset="134"size="100%">蘇艷芳</style></author></authors></contributors><titles><title><styleface="normal"font="default"charset="134"size="100%">火絨草屬植物的化學成分和藥理活性研究進展</style></title><secondary-title><styleface="normal"font="default"charset="134"size="100%">中國中藥雜志ChinaJournalofChineseMateriaMedica</style></secondary-title></titles><periodical><full-title>中國中藥雜志ChinaJournalofChineseMateriaMedica</full-title></periodical><pages>245-248</pages><volume>30</volume><number>4</number><dates><year>2005</year></dates><urls></urls></record></Cite></EndNote>[\o"武彥文,2005#59"15]?；鸾q草用于治療急慢性腎炎取得了較好的療效ADDINEN.CITE<EndNote><Cite><Author>常桂榮</Author><Year>1987</Year><RecNum>58</RecNum><DisplayText><styleface="superscript">[16]</style></DisplayText><record><rec-number>58</rec-number><foreign-keys><keyapp="EN"db-id="e2ep9dwvowt0f4eas51vzp24fwrrtpsar5fd">58</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author><styleface="normal"font="default"charset="134"size="100%">常桂榮</style></author></authors></contributors><titles><title><styleface="normal"font="default"charset="134"size="100%">中藥老頭草乙醇劑治療慢性腎炎45例</style></title><secondary-title><styleface="normal"font="default"charset="134"size="100%">中西醫(yī)結(jié)合雜志</style></secondary-title></titles><periodical><full-title>中西醫(yī)結(jié)合雜志</full-title></periodical><pages>669-670</pages><volume>7</volume><number>11</number><dates><year>1987</year></dates><urls></urls></record></Cite></EndNote>[\o"常桂榮,1987#58"16]。睢大員等研究表明火絨草水提液具有明顯的利尿作用ADDINEN.CITE<EndNote><Cite><Author>睢大員</Author><Year>2000</Year><RecNum>67</RecNum><DisplayText><styleface="superscript">[17]</style></DisplayText><record><rec-number>67</rec-number><foreign-keys><keyapp="EN"db-id="e2ep9dwvowt0f4eas51vzp24fwrrtpsar5fd">67</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author><styleface="normal"font="default"charset="134"size="100%">睢大員</style></author><author><styleface="normal"font="default"charset="134"size="100%">平立源</style></author><author><styleface="normal"font="default"charset="134"size="100%">于曉風</style></author><author><styleface="normal"font="default"charset="134"size="100%">呂忠智</style></author></authors></contributors><titles><title><styleface="normal"font="default"charset="134"size="100%">老頭草水提液利尿作用的初步研究</style></title><secondary-title><styleface="normal"font="default"charset="134"size="100%">人參研究RENSHENYANJIU</style></secondary-title></titles><periodical><full-title>人參研究RENSHENYANJIU</full-title></periodical><pages><styleface="normal"font="default"size="100%">24</style><styleface="normal"font="default"charset="134"size="100%">-27</style></pages><volume>12</volume><number>3</number><dates><year>2000</year></dates><urls></urls></record></Cite></EndNote>[\o"睢大員,2000#67"17]。宋雪英等發(fā)現(xiàn)火絨草水提物具有保肝作用ADDINEN.CITE<EndNote><Cite><Author>宋雪英</Author><Year>2010</Year><RecNum>65</RecNum><DisplayText><styleface="superscript">[18]</style></DisplayText><record><rec-number>65</rec-number><foreign-keys><keyapp="EN"db-id="e2ep9dwvowt0f4eas51vzp24fwrrtpsar5fd">65</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author><styleface="normal"font="default"charset="134"size="100%">宋雪英,</style></author><author><styleface="normal"font="default"charset="134"size="100%">伍義行,</style></author><author><styleface="normal"font="default"charset="134"size="100%">胡少青,</style></author><author><styleface="normal"font="default"charset="134"size="100%">郝冰潔</style></author><author><styleface="normal"font="default"charset="134"size="100%">孟慶莉</style></author></authors></contributors><titles><title><styleface="normal"font="default"charset="134"size="100%">火絨草水溶性提取物對D-半乳糖胺致肝細胞損傷的抑制作用</style></title><secondary-title><styleface="normal"font="default"charset="134"size="100%">世界華人消化雜志</style></secondary-title></titles><periodical><full-title>世界華人消化雜志</full-title></periodical><pages>3072-3077</pages><volume>18</volume><number>29</number><dates><year>2010</year></dates><urls></urls></record></Cite></EndNote>[\o"宋雪英,2010#65"18]。尹秀玲等對千名糖尿病患者服用火絨草白酒浸泡提取液發(fā)現(xiàn)火絨草治療糖尿病的有效率為100%。并證實火絨草含有的單體成分苯乙雙胍,具有降血糖作用ADDINEN.CITE<EndNote><Cite><Author>尹秀玲</Author><Year>2000</Year><RecNum>49</RecNum><DisplayText><styleface="superscript">[19]</style></DisplayText><record><rec-number>49</rec-number><foreign-keys><keyapp="EN"db-id="e2ep9dwvowt0f4eas51vzp24fwrrtpsar5fd">49</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author><styleface="normal"font="default"charset="134"size="100%">尹秀玲</style></author><author><styleface="normal"font="default"charset="134"size="100%">王金霞</style></author><author><styleface="normal"font="default"charset="134"size="100%">龍</style><styleface="normal"font="default"size="100%"></style><styleface="normal"font="default"charset="134"size="100%">茹</style></author></authors></contributors><titles><title><styleface="normal"font="default"charset="134"size="100%">火絨草治療糖尿病的研究與開發(fā)</style></title><secondary-title><styleface="normal"font="default"charset="134"size="100%">生物學雜志</style></secondary-title></titles><periodical><full-title>生物學雜志</full-title></periodical><pages>25-38</pages><volume>17</volume><number>2</number><dates><year>2000</year></dates><urls></urls></record></Cite></EndNote>[\o"尹秀玲,2000#49"19]。上述表明火絨草具有利尿、消炎、降血糖等顯著療效，藥性緩和且沒有毒副作用，民間用藥經(jīng)驗豐富，且生物活性廣泛。其野生資源極為豐富,而且具有廣泛的生理活性和豐富的民間用藥經(jīng)驗。因此,對該藥的化學成分和藥理活性深入研究和開發(fā)應(yīng)用具有廣闊的前景,有望開發(fā)出新的抗糖尿病和抗腎炎疾病的藥物。（三）香蕓火絨草化學成分的研究進展火絨草屬植物可以分離得到多種活性成分，迄今從中分離得到揮發(fā)油類、倍半萜類、苯丙素類、黃酮類、甾體類化合物。如今已經(jīng)從中獲取100余種揮發(fā)油類物質(zhì)、11種倍半萜類化合物、8種苯丙素類化合物、3種甾體類化合物（谷甾醇、菜油甾醇及豆甾醇）、9種黃酮類化合物ADDINEN.CITE<EndNote><Cite><Author>武彥文</Author><Year>2005</Year><RecNum>59</RecNum><DisplayText><styleface="superscript">[15]</style></DisplayText><record><rec-number>59</rec-number><foreign-keys><keyapp="EN"db-id="e2ep9dwvowt0f4eas51vzp24fwrrtpsar5fd">59</key></foreign-keys><ref-typename="JournalArticle">17</ref-type><contributors><authors><author><styleface="normal"font="default"charset="134"size="100%">武彥文</style></author><author><styleface="normal"font="default"charset="134"size="100%">高文遠</style></author><au