EMI56-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEEMI56Cat.No.:HY-131067CASNo.:2414374-41-5分?式:C??H??N?O?分?量:348.4作?靶點:EGFR作?通路:JAK/STATSignaling;ProteinTyrosineKinase/RTK儲存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實驗DMSO:50mg/mL(143.51mM;ultrasonicandwarmingandheatto60°C)MassSolvent1mg5mg10mgConcentration制備儲備液1mM2.8703mL14.3513mL28.7026mL5mM0.5741mL2.8703mL5.7405mL10mM0.2870mL1.4351mL2.8703mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時，請在6個?內(nèi)使?，-20°C儲存時，請在1個?內(nèi)使?。體內(nèi)實驗請根據(jù)您的實驗動物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實驗結(jié)果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:2.08mg/mL(5.97mM);Suspendedsolution;Needultrasonic1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEBIOLOGICALACTIVITY?物活性EMI56EMI1的衍?物，EMI56?EMI1對突變型EGFR具有更?的效?。EMI56抑制EGFR三重突變體[1]。IC50&TargetEGFRL858R/T790M/C797SEGFRdel19T790MC797S體外研究EMI56inhibitsEGFRex19del/T790M/C797SandEGFRL858R/T790M/C797S.EMI56canbeusedintheresearchofmutantEGFR-associated,drug-resistantnon-small-celllungcancer(NSCLC)[1].EMI56(10,15,20μM;2?hoftreatment)stronglyinhibitstotalEGFRlevels,activationanddownstreamsignalinginPC9EGFRex19del/T790M/C797Scells[1].CellViabilityAssay[1]CellLine:PC9EGFRex19del/T790M/C797ScellsConcentration:1,5,10,15,20μMIncubationTime:2?hoursResult:InhibitedtotalEGFRlevelsfrom10?μMconcentration.REFERENCES[1].PunitSaraon,etal.ADrugDiscoveryPlatformtoIdentifyCompoundsThatInhibitEGFRTripleMutants.NatChemBiol.2020May;16(5):577-586.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-