IQ-3-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEIQ-3Cat.No.:HY-107600CASNo.:312538-03-7分?式:C??H??N?O?分?量:341.32作?靶點(diǎn):JNK作?通路:MAPK/ERKPathway儲存?式:Powder-20°C3yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:25mg/mL(73.25mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM2.9298mL14.6490mL29.2980mL5mM0.5860mL2.9298mL5.8596mL10mM0.2930mL1.4649mL2.9298mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時，請在6個?內(nèi)使?，-20°C儲存時，請在1個?內(nèi)使?。BIOLOGICALACTIVITY?物活性IQ-3特異性的c-JNK家族抑制劑，對JNK3的選擇性較好。IQ-3對JNK1、JNK2和JNK3的Kd值分別為0.24μM、0.29μM和0.066μM。IC50&TargetJNK3JNK1JNK2CK1δ0.066μM(Kd)0.24μM(Kd)0.29μM(Kd)0.56μM(Kd)1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEPI3KγMKNK20.43μM(Kd)1.2μM(Kd)體外研究IQ-3exhibitsIC50of2.2μM(TNF-αinhumanmonoMac-6cells),1.5μM(IL-6inhumanmonoMac-6cells),4.7μM(TNF-αinhumanPBMCs),9.1μM(IL-6inhumanPBMCs)and6.1μM(NOinmurineJ774.A1),respectively[1].IQ-3exhibitsanIC50of1.4μMforinhibitingLPS-inducedNF-κB/AP-1transcriptionalactivityinhumanTHP-1Bluemonocyticcells[1].IQ-3isindeedacompetitiveinhibitorfortheATPbindingsiteofJNK3[1].CellViabilityAssay[1]CellLine:PBMCs.Concentration:0-80μM(200ng/mLLPS).IncubationTime:30min.Result:DownregulatedTNF-αconcentration(IC50=4.7μM).戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?JEthnopharmacol.2021Aug11;281:114438.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].IgorASchepetkin,etal.Identificationandcharacterizationofanovelclassofc-JunN-terminalkinaseinhibitors.MolPharmacol.2012Jun;81(6):832-45.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3792;