分子細胞遺傳學課程報告課件

1、Live imaging of rapid chromosome movements in meiotic prophase I in maizeWhy to research?The ability of chromosomes to move across the nuclear space is essential for the reorganization of the nucleus that takes place in early meiotic prophase. Chromosome dynamics of prophase I have been studied in b

2、udding and fission yeasts, but little is known about this process in higher eukaryotes.How to studyDifficulty :culture isolated live meiocytes of multicellular eukaryotesNew way: observe live meiocytes inside intact anthersresult1、maize chromosomes exhibited extremely dynamic and complex motility in

3、 zygonema and pachynema2 、The movement patterns differed dramatically between the two stages.3 、Chromosome movements included rotations of the entire chromatin and movements of individual chromosome segments4 、Chromosome motility was coincident with dynamic deformations of the nuclear envelope5 、 Bo

4、th, chromosome and nuclear envelope motility depended on actin microfilaments as well as tubulinMeiotic prophaseThree Chromosome Movement Classes in Zygotenecoordinated rotational movements of the entire chromatinrapid, short-distance oscillations of small individual chromosome segments extending fr

5、om the main chromatin mass into the nuclear spaceslower-paced movements of other chromosome segments mostly located inside the chromatin mass1、Three chromosome movement classes observed in a group of five zygotene meiocytes: rotational movements of the entire chromatin (yellow lines marking chromati

6、n mass edges), rapid, short-distance movements of small chromosome segments (blue and green), slower-paced movements of chromosome segments inside the chromatin mass (red). 2、 Cumulative tracks marking rotational movements of the entire chromatin in the two meiocytes traced in A after145 s. Starting

7、 and ending positions of the rotating chromatin in the nuclei from B and D.3、 Cumulative tracks of the small chromosomesegments tracked in the two meiocytes in A after 145 sTransition -from Zygonema to Pachynem a period of quiescence- only small-angle rotations of the entire chromatin mass (Movie S2

8、2). Only a few chromosome segments were moving, and the magnitude and speed of these movements was reduced compared with zygonema.Dynamic changes of the NE shape in zygonemaWhich lead they move Actin and Tubulin Are Both Required for Meiotic Prophase Chromosome Movements.examine- An hour-long incubation of anthers in latrunculin B (at concentrations 0.5 or 1M) or colc