Pyridostatin-hydrochloride-RR-82-hydrochloride-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEPyridostatin hydrochlorideCat. No.: HY-15176ACAS No.: 1781882-65-2Synonyms: RR-82 hydrochloride分式: CHClNO分量: 778.94作靶點(diǎn): G-quadruplex作通路: Cell Cycle/DNA Damage儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month

2、溶解性數(shù)據(jù)體外實(shí)驗(yàn) H2O : 31 mg/mL (39.80 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 1.2838 mL 6.4190 mL 12.8380 mL5 mM 0.2568 mL 1.2838 mL 2.5676 mL10 mM 0.1284 mL 0.6419 mL 1.2838 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Pyridost

3、atin hydrochloride種 G-quadruplexe 穩(wěn)定劑，Kd 值為 490 nM。IC50 & Target Kd: 490 nM (G-quadruplexe) 1體外研究Pyridostatin hydrochloride is a G-quadruplexe stabilizer, with a Kd of 490 nM 1. Pyridostatin (PDS) shows1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEneurotoxic activity against primary cortical neur

4、ons at 0.01-5 M, causes DNA double-strand breaks (DSBs)at 1 M, downregulates BRCA1 in neurons at 1, 2 or 5 M 2. Pyridostatin interacts with G-quadruplex motifsin SRC and alters mRNA levels of damaged genes 3.PROTOCOLCell Assay 3 Cells are plated at equal confluence and either untreated or treated wi

5、th 2 M Pyridostatin continually for 72h. Cells from individual plates are trypsinized and counted in a Coultercounter. Graphs represent total cellnumbers at each time interval and error bars represent S.E.M. Data represent three independentexperiments 3.MCE has not independently confirmed the accura

6、cy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) Nucleic Acids Res. 2019 Jun 5. pii: gkz484. J Mol Med (Berl). 2019 Jun 14.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Koirala D, et al. A single-molecule platform for investigation of interactions between G-quadru

7、plexes and small-molecule ligands. NatChem. 2011 Aug 28;3(10):782-7.2. Moruno-Manchon JF, et al. The G-quadruplex DNA stabilizing drug pyridostatin promotes DNA damage and downregulates transcriptionof Brca1 in neurons. Aging (Albany NY). 2017 Sep 12;9(9):1957-1970.3. Rodriguez R, et al. Small-molecule-induced DNA damage identifies alternative DNA structures in human genes. Nat Chem Biol. 2012Feb 5;8(3):301-10.McePdfHeightCaution: Product has not been fully validated for medical applications.For rese