CAY10602 - Sirtuin Activator - 生命科學(xué)試劑 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemECAY10602Cat. No.: HY-104073CAS No.: 374922-43-7分式: CHFNOS分量: 418.44作靶點(diǎn): Sirtuin作通路: Cell Cycle/DNA Damage; Epigenetics儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 100 mg/mL (238.98 mM; N

2、eed ultrasonic and warming)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 2.3898 mL 11.9491 mL 23.8983 mL5 mM 0.4780 mL 2.3898 mL 4.7797 mL10 mM 0.2390 mL 1.1949 mL 2.3898 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存式和期限。體內(nèi)實(shí)驗(yàn) 請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥式選擇適當(dāng)?shù)娜芙獍?，配制前?qǐng)先配制澄清的儲(chǔ)備液，再依次添加助溶劑(為保證實(shí)驗(yàn)結(jié)果的可靠性，體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)

3、天使；澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占)：1. 請(qǐng)依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (5.97 mM); Clear solution2. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (5.97 mM); Precipitated solutionBIOLOGICAL ACTIVITY1/2 Master of Small Molecules 您邊的抑制劑師ww

4、w.MedChemE物活性 CAY10602個(gè) SIRT1 激活劑。IC50 & Target SIRT1體外研究 Compounds (including CAY10602/compound 1) with SIRT1-activating properties have a significant effect onfat mobilization in differentiated adipocytes, and these compounds (including CAY10602) have antiobesityand/or antidiabetic properties. Com

5、pounds (including CAY10602) with SIRT1 activating potential exert strongsuppression of TNF- release at concentrations between 20 and 60 M 1.PROTOCOLCell Assay 1 3T3L1 mouse fibroblasts and THP-1 leukemia cells are used and cultured following the suppliersrecommendations.THP-1 cells are treated first

6、 with representative compounds (includingCAY10602/compound 1) followed by addition of lipopolysaccharide(LPS). THP-1 cells are plated at a densityof 1.2106 cells/mL in 0.4 mL (0.5106 cells/treatment) in 24-well culture plates. The cells are preincubatedwith representative test compounds (including C

7、AY10602) at 2 concentrations (20 or 60 M) for 1 h undergeneral tissue culture conditions 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) Cell Death Dis. 2019 Apr 15;10(5):331. Toxicol Appl Pharmacol. 2019 May 11.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Nayagam VM, et al. SIRT1 modulating compounds from high-throughput screening as anti-inflammatory and insulin-sensitizing agents. JBiomol Screen. 2006 Dec;11(8):959-67.McePdfHeightCaution: Product has not been fully validated for medical applications.For